Rice mitochondria sterility gene and application thereof

Abstract

The invention relates to the technical field of rice breeding, and specifically relates to a rice mitochondria sterility gene and the application thereof. The invention discloses a rice mitochondria sterility gene and a coding sequence thereof, and D1 type cytoplasm can be quickly and efficiently screened and authenticated from wild rice for cultivation of a D1 type cytoplasm sterile line by designing a specific molecular marker through the gene sequence. A mitochondrial genome specific sequence of the D1 type cytoplasm sterile is authenticated through comparative genomics, sterility related ORFs are authenticated through gene predication and differential expression analysis, and a plant transformation vecter is constructed for transforming maintenance line verification and sterility function.

Description

technical field [0001] The invention relates to the technical field of rice breeding, in particular to a rice mitochondrial sterility gene and its application. Background technique [0002] In the 1970s, my country successfully applied the three-line method to cultivate hybrid rice, and promoted the planting on a large scale in production, realizing the second leap in my country's rice yield per unit area. From 1976 to 2005, the accumulative planting area of ​​hybrid rice in my country was 5.25 billion mu, and the rice harvest increased by about 650 million tons. Therefore, the development of hybrid rice is an important way to increase food production and solve food security problems (Yuan Longping, 2008). The basis for the use of three-line hybrid rice is cytoplasmic male sterility. Since the discovery of the wild-type cytoplasmic male sterile line and its widespread application, from the 1970s to the mid-1980s, breeders have bred as many as 60 kinds of cytoplasmic male ste...

AI Technical Summary

Problems solved by technology

[0005] However, the D1 cytoplasmic sterile gene has not been cloned so far

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