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DNA detection method for detecting chest depth traits of Luxi blackhead rams and application of DNA detection method

A detection method, Luxihei's technology, applied in the field of molecular biology, can solve the problems of less research and achieve the effects of accelerating the establishment, improving the speed of breeding of improved varieties, and low cost

Active Publication Date: 2020-05-08
INST OF ANIMAL SCI & VETERINARY MEDICINE SHANDONG ACADEMY OF AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, what kind of function it has is still unknown, and there are still relatively few studies on the association analysis of these InDel sites with animal growth and reproductive traits
However, there is no report on the significant correlation between the InDel locus of the IGF2BP1 gene and the deep chest growth traits of sheep

Method used

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  • DNA detection method for detecting chest depth traits of Luxi blackhead rams and application of DNA detection method
  • DNA detection method for detecting chest depth traits of Luxi blackhead rams and application of DNA detection method
  • DNA detection method for detecting chest depth traits of Luxi blackhead rams and application of DNA detection method

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Experimental program
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Embodiment 1

[0025] The present invention utilizes the PCR method to detect the insertion / deletion polymorphism that may be produced by the mutation of the fourth intron region of the sheep IGF2BP1 gene (reference sequence: NC_019468.1), and compares it with the breast depth traits of sheep and rams. Conduct association analysis to verify whether there are molecular markers that can be used as auxiliary selection in sheep molecular breeding.

[0026] 1. Experimental drugs and reagents

[0027] 1.1 Biochemical reagents and biological reagents: ①Taq DNA polymerase (purchased from Fermantas or MBI Company); ②Proteinase K (purchased from Huamei Bioengineering Company); ③Marker I (purchased from Tiangen Biochemical Technology (Beijing) Co., Ltd.).

[0028] 1.2 Common reagents: Tris, EDTA, NaCl, HCl, NaOH, Tris saturated phenol, chloroform, absolute ethanol, sodium dodecylsulfonate (SDS), ethidium bromide (EB), bromophenol blue, dimethyl Common reagents such as benzocyanide FF, boric acid, agar...

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Abstract

The invention discloses a DNA detection method for detecting chest depth traits of Luxi blackhead rams and an application of the DNA detection method. The method comprises the following steps: by using whole genome DNA of the Luxi blackhead rams to be tested as a template and a primer pair P1 as amplification primers, performing amplification on a fragment containing an insertion / deletion polymorphic site of a fourth intron region of a sheep IGF2BP1 gene by using PCR, performing electrophoresis on PCR amplification products, and screening Luxi blackhead rams having excellent chest depth traitsaccording to the electrophoresis results. The method can use the IGF2BP1 gene to establish a kit for rapid identification of the Luxi blackhead ram genetic resource populations having the excellent chest depth traits, and can screen the sheep genetic resource populations having the excellent chest depth traits.

Description

technical field [0001] The invention relates to a DNA detection method for detecting breast depth traits of Luxi black rams and an application thereof, belonging to the field of molecular biology. Background technique [0002] With the development of the economy and the improvement of people's living standards, people's choices of meat products tend to be more diversified, and people gradually focus on whether they have high nutritional characteristics. Therefore, mutton has attracted people's attention because of its delicious meat, high protein, low fat and other advantages. In the field of animal science research, in order to improve the meat yield of sheep, how to select sheep individuals with better growth traits has always been a key issue in animal genetics and breeding. [0003] With the further improvement of genetic maps of various species, the completion of genome sequencing and the rapid development of molecular biology, molecular breeding will be combined with ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6888C12Q1/6858C12N15/11
CPCC12Q1/6888C12Q1/6858C12Q2600/124C12Q2600/156C12Q2600/172C12Q2531/113C12Q2565/125
Inventor 宋恩亮刘洪飞姜富贵蓝贤勇成海建张果平魏晨毛翠
Owner INST OF ANIMAL SCI & VETERINARY MEDICINE SHANDONG ACADEMY OF AGRI SCI
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