Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Primer pairs for detecting insertion/deletion polymorphism of sheep BMPR1B gene and kit, method and application

A technology for polymorphism detection and detection methods, applied in biochemical equipment and methods, microbial measurement/testing, DNA/RNA fragments, etc., can solve problems such as undiscovered patent documents, and achieve improved breeding speed, Faster set-up, low-cost results

Pending Publication Date: 2020-05-15
天津奥群牧业有限公司
View PDF1 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Through searching, no published patent documents related to this patent application have been found

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Primer pairs for detecting insertion/deletion polymorphism of sheep BMPR1B gene and kit, method and application
  • Primer pairs for detecting insertion/deletion polymorphism of sheep BMPR1B gene and kit, method and application
  • Primer pairs for detecting insertion/deletion polymorphism of sheep BMPR1B gene and kit, method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0030] The embodiments of the present invention will be described in detail below. It should be noted that the embodiments are illustrative, not restrictive, and cannot limit the protection scope of the present invention.

[0031] The raw materials used in the present invention, unless otherwise specified, are conventional commercially available products; the methods used in the present invention, unless otherwise specified, are conventional methods in the art.

[0032] A pair of primers for detecting insertion / deletion polymorphism of sheep BMPR1B gene, said primer pair can amplify the fragment containing the insertion / deletion polymorphism site in the intron region of sheep BMPR1B gene by PCR.

[0033]Preferably, the insertion / deletion polymorphic site is a 10-bp insertion / deletion polymorphic site at position NC_040257.1:g29373339_29373348 of the sheep BMPR1B gene.

[0034] Preferably, the primer pair is:

[0035] Upstream primer: SEQ NO.15'-CAACCTCATCTCTTGGCCCT-3'(20nt); ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a method for detecting insertion / deletion polymorphism of a sheep BMPR1B gene by using primer pairs. The detection method comprises the following steps: by taking sheep genomeDNA (deoxyribonucleic acid) to be detected as a template, and the primer pairs as amplification primers, performing PCR (polymerase chain reaction) amplification on a segment with insertion / deletionpolymorphism sites in a sheep BMPR1B gene intron region, performing electrophoresis on an amplification product, and identifying genotypes of the insertion / deletion polymorphism sites according to electrophoresis results. By adopting the method provided by the invention, the primers are designed according to the insertion / deletion polymorphism sites (refer to a sequence NC_040257.1:g29373339_29373348) in the sheep BMPR1B gene intron region, the sheep genome DNA is adopted as the temperature, and the genotypes of the insertion / deletion polymorphism sites can be simply, rapidly and precisely detected with low cost through sequence amplification and electrophoresis identification.

Description

technical field [0001] The invention belongs to the technical field of biotechnology and livestock breeding, in particular to a primer pair, kit, method and application for detecting insertion / deletion polymorphism of sheep BMPR1B gene. Background technique [0002] Sheep are deeply loved by consumers for its delicious mutton meat, low fat, and low cholesterol. With the improvement of people's living standards, the society's demand for mutton, goat milk, cashmere and other products is increasing. In order to achieve the goal of high-yield, high-quality and efficient sheep breeding, people have studied sheep at the molecular level, using DNA polymorphisms as markers to conduct genetic analysis and research on the gene structure and function of sheep, and through screening and sheep production. DNA marker loci closely related to litter size traits are detected, and according to the association between their gene polymorphisms and litter size traits, the establishment of sheep ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6888C12Q1/6858C12N15/11
CPCC12Q1/6888C12Q1/6858C12Q2600/124C12Q2600/156C12Q2531/113C12Q2565/125Y02P60/87
Inventor 张清峰蓝贤勇李海霞潘传英林春建段崇杰郝坤杰卢小芳
Owner 天津奥群牧业有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com