Primer pairs for detecting insertion/deletion polymorphism of sheep BMPR1B gene and kit, method and application

A technology for polymorphism detection and detection methods, applied in biochemical equipment and methods, microbial measurement/testing, DNA/RNA fragments, etc., can solve problems such as undiscovered patent documents, and achieve improved breeding speed, Faster set-up, low-cost results

Pending Publication Date: 2020-05-15
天津奥群牧业有限公司
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[0006] Through searching, no published patent documents related to this patent application have been found

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  • Primer pairs for detecting insertion/deletion polymorphism of sheep BMPR1B gene and kit, method and application
  • Primer pairs for detecting insertion/deletion polymorphism of sheep BMPR1B gene and kit, method and application
  • Primer pairs for detecting insertion/deletion polymorphism of sheep BMPR1B gene and kit, method and application

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Embodiment Construction

[0030] The embodiments of the present invention will be described in detail below. It should be noted that the embodiments are illustrative, not restrictive, and cannot limit the protection scope of the present invention.

[0031] The raw materials used in the present invention, unless otherwise specified, are conventional commercially available products; the methods used in the present invention, unless otherwise specified, are conventional methods in the art.

[0032] A pair of primers for detecting insertion / deletion polymorphism of sheep BMPR1B gene, said primer pair can amplify the fragment containing the insertion / deletion polymorphism site in the intron region of sheep BMPR1B gene by PCR.

[0033]Preferably, the insertion / deletion polymorphic site is a 10-bp insertion / deletion polymorphic site at position NC_040257.1:g29373339_29373348 of the sheep BMPR1B gene.

[0034] Preferably, the primer pair is:

[0035] Upstream primer: SEQ NO.15'-CAACCTCATCTCTTGGCCCT-3'(20nt); ...

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Abstract

The invention relates to a method for detecting insertion / deletion polymorphism of a sheep BMPR1B gene by using primer pairs. The detection method comprises the following steps: by taking sheep genomeDNA (deoxyribonucleic acid) to be detected as a template, and the primer pairs as amplification primers, performing PCR (polymerase chain reaction) amplification on a segment with insertion / deletionpolymorphism sites in a sheep BMPR1B gene intron region, performing electrophoresis on an amplification product, and identifying genotypes of the insertion / deletion polymorphism sites according to electrophoresis results. By adopting the method provided by the invention, the primers are designed according to the insertion / deletion polymorphism sites (refer to a sequence NC_040257.1:g29373339_29373348) in the sheep BMPR1B gene intron region, the sheep genome DNA is adopted as the temperature, and the genotypes of the insertion / deletion polymorphism sites can be simply, rapidly and precisely detected with low cost through sequence amplification and electrophoresis identification.

Description

technical field [0001] The invention belongs to the technical field of biotechnology and livestock breeding, in particular to a primer pair, kit, method and application for detecting insertion / deletion polymorphism of sheep BMPR1B gene. Background technique [0002] Sheep are deeply loved by consumers for its delicious mutton meat, low fat, and low cholesterol. With the improvement of people's living standards, the society's demand for mutton, goat milk, cashmere and other products is increasing. In order to achieve the goal of high-yield, high-quality and efficient sheep breeding, people have studied sheep at the molecular level, using DNA polymorphisms as markers to conduct genetic analysis and research on the gene structure and function of sheep, and through screening and sheep production. DNA marker loci closely related to litter size traits are detected, and according to the association between their gene polymorphisms and litter size traits, the establishment of sheep ...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6888C12Q1/6858C12N15/11
CPCC12Q1/6888C12Q1/6858C12Q2600/124C12Q2600/156C12Q2531/113C12Q2565/125
Inventor 张清峰蓝贤勇李海霞潘传英林春建段崇杰郝坤杰卢小芳
Owner 天津奥群牧业有限公司
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