A DNA detection method and its application for detecting breast depth traits of Luxi black rams

A detection method, Luxihei's technology, applied in the field of molecular biology, can solve the problems of less research and achieve the effect of speeding up establishment, low cost, and improving the speed of breeding of improved varieties

Active Publication Date: 2022-02-25
INST OF ANIMAL SCI & VETERINARY MEDICINE SHANDONG ACADEMY OF AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, what kind of function it has is still unknown, and there are still relatively few studies on the association analysis of these InDel sites with animal growth and reproductive traits
However, there is no report on the significant correlation between the InDel locus of the IGF2BP1 gene and the deep chest growth traits of sheep

Method used

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  • A DNA detection method and its application for detecting breast depth traits of Luxi black rams
  • A DNA detection method and its application for detecting breast depth traits of Luxi black rams
  • A DNA detection method and its application for detecting breast depth traits of Luxi black rams

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Experimental program
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Embodiment 1

[0025] The present invention utilizes the PCR method to detect the insertion / deletion polymorphism that may be produced by the mutation of the fourth intron region of the sheep IGF2BP1 gene (reference sequence: NC_019468.1), and compares it with the breast depth traits of sheep and rams. Conduct association analysis to verify whether there are molecular markers that can be used as auxiliary selection in sheep molecular breeding.

[0026] 1. Experimental drugs and reagents

[0027] 1.1 Biochemical reagents and biological reagents: ①Taq DNA polymerase (purchased from Fermantas or MBI Company); ②Proteinase K (purchased from Huamei Bioengineering Company); ③Marker I (purchased from Tiangen Biochemical Technology (Beijing) Co., Ltd.).

[0028] 1.2 Common reagents: Tris, EDTA, NaCl, HCl, NaOH, Tris saturated phenol, chloroform, absolute ethanol, sodium dodecylsulfonate (SDS), ethidium bromide (EB), bromophenol blue, dimethyl Common reagents such as benzocyanide FF, boric acid, agar...

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Abstract

The invention discloses a DNA detection method for detecting breast depth traits of Luxi black ram and its application. The whole genome DNA of the Luxi black ram to be tested is used as a template, and the primer pair P1 is used as an amplification primer to amplify by PCR The fragment containing the insertion / deletion polymorphic site in the fourth intron region of the sheep IGF2BP1 gene was electrophoresed on the PCR amplification product, and the Luxi black ram with excellent breast depth was screened according to the electrophoresis results. This method can not only The IGF2BP1 gene was used to establish a population for rapid identification of Luxi black ram genetic resources with excellent chest depth traits, and at the same time to screen sheep genetic resource populations with excellent chest depth traits.

Description

technical field [0001] The invention relates to a DNA detection method for detecting breast depth traits of Luxi black rams and an application thereof, belonging to the field of molecular biology. Background technique [0002] With the development of the economy and the improvement of people's living standards, people's choices of meat products tend to be more diversified, and people gradually focus on whether they have high nutritional characteristics. Therefore, mutton has attracted people's attention because of its delicious meat, high protein, low fat and other advantages. In the field of animal science research, in order to improve the meat yield of sheep, how to select sheep individuals with better growth traits has always been a key issue in animal genetics and breeding. [0003] With the further improvement of genetic maps of various species, the completion of genome sequencing and the rapid development of molecular biology, molecular breeding will be combined with ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6888C12Q1/6858C12N15/11
CPCC12Q1/6888C12Q1/6858C12Q2600/124C12Q2600/156C12Q2600/172C12Q2531/113C12Q2565/125
Inventor 宋恩亮刘洪飞姜富贵蓝贤勇成海建张果平魏晨毛翠
Owner INST OF ANIMAL SCI & VETERINARY MEDICINE SHANDONG ACADEMY OF AGRI SCI
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