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Primer pair, kit and method for detecting sheep CSF1R gene insertion/deletion polymorphism and application of method

A technology for polymorphism detection and detection methods, applied in biochemical equipment and methods, microbial measurement/inspection, DNA/RNA fragments, etc., can solve the problems of undiscovered patent documents, etc., to improve the speed of breeding of improved varieties, The effect of low cost and accelerated cultivation

Pending Publication Date: 2020-05-08
天津奥群牧业有限公司
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[0006] Through searching, no published patent documents related to this patent application have been found

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  • Primer pair, kit and method for detecting sheep CSF1R gene insertion/deletion polymorphism and application of method
  • Primer pair, kit and method for detecting sheep CSF1R gene insertion/deletion polymorphism and application of method
  • Primer pair, kit and method for detecting sheep CSF1R gene insertion/deletion polymorphism and application of method

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Embodiment Construction

[0028] The embodiments of the present invention will be described in detail below. It should be noted that the embodiments are descriptive, not restrictive, and cannot limit the protection scope of the present invention.

[0029] The raw materials used in the present invention are conventional commercial products unless otherwise specified; the methods used in the present invention are conventional methods in the art unless otherwise specified.

[0030] A primer pair for detection of ovine CSF1R gene insertion / deletion polymorphism, the primer pair can be used for PCR amplification of a fragment comprising an ovine CSF1R gene intron region insertion / deletion polymorphism site.

[0031] Preferably, the insertion / deletion polymorphism site is a 27-bp insertion / deletion polymorphism site at position NC_040256.1:g.13016-13043 of the sheep CSF1R gene.

[0032] Preferably, the primer pair is:

[0033] Upstream primer: SEQ NO.1: 5'-TCCTCCAGTTCCAACAGAAAACC-3';

[0034] Downstream pr...

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Abstract

The invention relates to a primer pair, kit and method for detecting sheep colony stimulating factor1 receptor (CSF1R) gene insertion / deletion polymorphism and an application of the method. The primerpair can be used for PCR amplification of a fragment containing sheep CSF1R gene intron region insertion / deletion polymorphic loci. The primers can be designed according to the sheep CSF1R gene intron region insertion / deletion polymorphic loci, genotypes of the insertion / deletion polymorphic loci can be detected simply, quickly, at low cost and accurately by using sheep genome DNA as a template through sequence amplification and electrophoretic identification, and different genotypes of the 27-bp insertion / deletion polymorphic loci have a significant correlation with mastitis traits of Australian white sheep. The method for detecting the sheep CSF1R gene insertion / deletion polymorphism provided by the invention can be applied to sheep molecular marker assisted selection breeding to accelerate the cultivation of sheep populations with high resistance to mastitis.

Description

technical field [0001] The invention belongs to the technical field of biotechnology and livestock breeding, in particular to a primer pair, kit, method and application for detecting insertion / deletion polymorphism of sheep CSF1R gene. Background technique [0002] Sheep are deeply loved by consumers for their meat, milk and cashmere properties. With the improvement of people's living standards, the society's demand for mutton, goat milk, cashmere and other products is getting higher and higher. In order to achieve high-yield, high-quality and high-efficiency sheep breeding, people's research on sheep has carried out genetic analysis and research on the genetic structure and function of sheep at the molecular level, using DNA polymorphisms as markers. DNA markers closely related to inflammatory traits are detected and detected, and based on the association between their gene polymorphisms and mastitis traits, the establishment of a sheep population with high mastitis resista...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6883C12Q1/6858C12N15/11
CPCC12Q1/6883C12Q1/6858C12Q2600/156C12Q2531/113C12Q2565/125
Inventor 张清峰户会娜程志强潘传英林春建尹静韩旭飞卢小芳郝坤杰蓝贤勇
Owner 天津奥群牧业有限公司
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