Detection primer pair, kit and method for sheep GHR gene insertion/deletion polymorphism and application of method

A technology for polymorphism detection and detection methods, applied in biochemical equipment and methods, microbiological determination/inspection, DNA/RNA fragments, etc., can solve problems such as undiscovered public patent documents, and achieve accelerated litter size and excellent traits The effect of high-quality, low-cost, and improved breeding speed

Pending Publication Date: 2020-05-08
天津奥群牧业有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] Through searching, no published patent docume

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  • Detection primer pair, kit and method for sheep GHR gene insertion/deletion polymorphism and application of method
  • Detection primer pair, kit and method for sheep GHR gene insertion/deletion polymorphism and application of method
  • Detection primer pair, kit and method for sheep GHR gene insertion/deletion polymorphism and application of method

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Embodiment Construction

[0031] The embodiments of the present invention will be described in detail below. It should be noted that the embodiments are illustrative, not restrictive, and cannot limit the protection scope of the present invention.

[0032] The raw materials used in the present invention, unless otherwise specified, are conventional commercially available products; the methods used in the present invention, unless otherwise specified, are conventional methods in the art.

[0033] A pair of primers for detecting insertion / deletion polymorphism of sheep GHR gene, said primer pair can be used for PCR amplification of fragments containing insertion / deletion polymorphism sites in the intron region of sheep GHR gene.

[0034]Preferably, the insertion / deletion polymorphic site is a 23-bp insertion / deletion polymorphic site at position NC_040267.1:g.32005124_32005146 of the sheep GHR gene.

[0035] Preferably, the primer pair is:

[0036] Upstream primer: SEQ NO.1 5'-TGCTGTATGGCCCCTCTAGTA-3'(2...

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Abstract

The invention relates to a primer pair for detecting sheep growth hormone receptor (GHR) gene insertion/deletion polymorphism. The primer pair can be used for PCR amplification of a fragment containing a sheep GHR gene intron region insertion/deletion polymorphic site. The method can detect the genotype of the insertion/deletion polymorphic site simply, quickly, at low cost and accurately, and thedetected insertion/deletion polymorphic site can be used as a molecular marker site for the number of lambs produced by sheep, so that the establishment of sheep populations with excellent litter size traits can be accelerated, and the speed of improved variety breeding can be improved.

Description

technical field [0001] The invention belongs to the technical field of biotechnology and domestic animal breeding, in particular to a detection primer pair, kit, method and application of sheep GHR gene insertion / deletion polymorphism. Background technique [0002] Sheep are deeply loved by consumers for their meat, milk and cashmere production properties. With the improvement of people's living standards, the society's demand for mutton, goat milk, cashmere and other products is getting higher and higher. In order to achieve the goal of high-yield, high-quality and efficient sheep breeding, people have studied sheep at the molecular level, using DNA polymorphisms as markers to conduct genetic analysis and research on the gene structure and function of sheep, and through screening and sheep production. DNA marker loci closely related to litter size traits are detected, and according to the association between their gene polymorphisms and litter size traits, the establishment...

Claims

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Application Information

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IPC IPC(8): C12Q1/6888C12Q1/6858C12N15/11
CPCC12Q1/6888C12Q1/6858C12Q2600/124C12Q2600/156C12Q2531/113C12Q2565/125
Inventor 张清峰蓝康澍曹如潘传英林春建尹静李飞赵军军赵京峰蓝贤勇
Owner 天津奥群牧业有限公司
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