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The method and application of detection of insertion-deletion polymorphism of sheep fth-1 gene by pcr-sscp

A FTH-1, indel technology, applied in the field of molecular genetics, can solve problems such as lack of research

Active Publication Date: 2020-06-09
甘肃润牧生物工程有限责任公司
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Problems solved by technology

Due to the lack of research in the field of sheep FTH-1 gene genetic variation, the research on the function of this gene locus and the research on the relationship between the genetic variation of this gene and reproductive traits have become blank

Method used

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  • The method and application of detection of insertion-deletion polymorphism of sheep fth-1 gene by pcr-sscp
  • The method and application of detection of insertion-deletion polymorphism of sheep fth-1 gene by pcr-sscp
  • The method and application of detection of insertion-deletion polymorphism of sheep fth-1 gene by pcr-sscp

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Embodiment Construction

[0027] The present invention will be described in detail below in conjunction with the accompanying drawings and embodiments.

[0028] In the present invention, primers are designed to amplify the 236bp fragment of the FTH-1 gene with the conserved sequence of the FTH-1 gene, and the sheep genome is used as a template to carry out PCR amplification, and the amplified product is sequenced to search for the insertion-deletion polymorphism of the amplified fragment; for the discovery The indel polymorphism of the FTH-1 gene can be quickly and easily detected as a molecular genetic marker, and it can provide a basis for speeding up the establishment of sheep populations with high-quality economic traits. in accordance with.

[0029] a. Detection of sheep FTH-1 gene polymorphism

[0030] 1. Collection and processing of sheep blood samples

[0031] Take 10 mL of blood samples from Small-tailed Han sheep and Hu sheep respectively, add 500 μL of 0.5 mol / L EDTA to anticoagulate, slow...

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Abstract

The invention discloses a method for detecting insertion-deletions polymorphism of sheep FTH-1 (ferritin heavy polypeptide1) genes by utilizing PCR-SSCP (polymerase chain reaction-single strand conformation polymorphism) and application thereof. The method comprises the following steps: taking to-be-detected sheep whole genome DNA containing FTH-1 genes as a template, performing PCR amplification on the sheep FTH-1 gene segments, and performing detection and genotyping on the PCR product by utilizing the SSCP technology; and identifying the 639th locus insertion-deletions polymorphism of the sheep FTH-1 genes according to electrophoretic results. The method is a method for screening and detecting a molecular genetic marker which is closely related to reproductive performances of sheep on the DNA level so as to be used for assistant selection and molecular breeding of the sheep and acceleration of sheep stock breeding.

Description

technical field [0001] The invention belongs to the field of molecular genetics, and relates to using the insertion-deletion (indels) of sheep functional genes as molecular genetic markers, in particular to the 4bp insertion-deletion (indels) polymorphism of the FTH-1 gene of Small Tail Han sheep and Hu sheep and its detection method. Background technique [0002] In sheep breeding, it is expected that through the selection of DNA markers that are closely related to reproductive traits and closely linked to quantitative traits, the purpose of early selection and improvement of the accuracy of breeding values ​​can be achieved, so as to obtain greater heredity in livestock and poultry breeding. progress. [0003] Molecular breeding, that is, Molecular Mark-Assist Selection (MAS), is a technology that uses DNA molecular markers to select genetic resources or breeding materials, and to improve the comprehensive traits of livestock and poultry. It uses modern molecular The met...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6888C12Q1/6858
CPCC12Q1/6858C12Q1/6888C12Q2600/156C12Q2531/113C12Q2565/131
Inventor 乐祥鹏裴生伟吕佳颖李发弟李万宏王维民
Owner 甘肃润牧生物工程有限责任公司
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