Primer pair, kit and method for detecting insertion / deletion polymorphism of sheep PRL gene and application of primer pair, kit and method

A polymorphism detection and detection method technology, applied in biochemical equipment and methods, microbial determination/inspection, DNA/RNA fragments, etc., can solve the problem of undiscovered patent documents, etc., to accelerate the lambing number and excellent traits The effect of speeding up the establishment and improving the breeding speed of fine varieties

Active Publication Date: 2020-05-01
天津奥群牧业有限公司
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  • Abstract
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[0006] Through searching, no published patent docume

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  • Primer pair, kit and method for detecting insertion / deletion polymorphism of sheep PRL gene and application of primer pair, kit and method
  • Primer pair, kit and method for detecting insertion / deletion polymorphism of sheep PRL gene and application of primer pair, kit and method
  • Primer pair, kit and method for detecting insertion / deletion polymorphism of sheep PRL gene and application of primer pair, kit and method

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Embodiment Construction

[0031] The embodiments of the present invention will be described in detail below. It should be noted that the embodiments are illustrative, not restrictive, and cannot limit the protection scope of the present invention.

[0032] The raw materials used in the present invention, unless otherwise specified, are conventional commercially available products; the methods used in the present invention, unless otherwise specified, are conventional methods in the art.

[0033] A pair of primers for detecting insertion / deletion polymorphisms of sheep PRL gene, said primer pair can be used for PCR amplification of fragments containing insertion / deletion polymorphism sites in the intron region of sheep PRL genes.

[0034] Preferably, the insertion / deletion polymorphic site is a 23-bp insertion / deletion polymorphic site at position NC_040271.1:g.3422967_34262989 of sheep PRL gene.

[0035] Preferably, the primer pair is:

[0036] Upstream primer: SEQ NO.1 5'-GGGAAGGGAAGAGAAACAGAGG-3'(22nt...

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Abstract

The invention relates to a primer pair for detecting insertion / deletion polymorphism of a sheep PRL gene. The primer pair can be used for PCR amplification of a fragment containing an insertion / deletion polymorphism site of an intron region of the sheep PRL gene. According to the primer pair, genotype of the insertion/deletion polymorphism site can be simply, quickly and accurately detected atlow cost. The detected insertion/deletion polymorphism site can be used as a molecular marker site for number of lambs produced by sheep, thereby accelerating an establishment of a sheep population with excellent lamb number traits and improving the breeding speed of improved varieties.

Description

technical field [0001] The invention belongs to the technical field of biotechnology and livestock breeding, in particular to a detection primer pair, kit, method and application of sheep PRL gene insertion / deletion polymorphism. Background technique [0002] Sheep are deeply loved by consumers for their meat, milk and cashmere production properties. With the improvement of people's living standards, the society's demand for mutton, goat milk, cashmere and other products is getting higher and higher. In order to achieve the goal of high-yield, high-quality and efficient sheep breeding, people have studied sheep at the molecular level, using DNA polymorphisms as markers to conduct genetic analysis and research on the gene structure and function of sheep, and through screening and sheep production. DNA marker loci closely related to litter size traits are detected, and according to the association between their gene polymorphisms and litter size traits, the establishment of sh...

Claims

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Application Information

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IPC IPC(8): C12Q1/6888C12Q1/6858C12N15/11
CPCC12Q1/6888C12Q1/6858C12Q2600/156C12Q2600/124C12Q2531/113C12Q2565/125Y02P60/87
Inventor 张清峰蓝康澍曹如潘传英林春建王权锋李若亮兰天鑫蓝贤勇
Owner 天津奥群牧业有限公司
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