Reaction system and kit for analyzing moringa oleifera genetic relationship and application method thereof

A kind of kinship and reaction system technology, applied in the field of biotechnology research, can solve the problem of the large influence of SRAP molecular markers, and achieve the effect of shortening the experimental time, low cost and high polymorphism

Active Publication Date: 2018-08-28
FUJIAN INST OF TROPICAL CROPS
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  • Abstract
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Problems solved by technology

However, SRAP molecular markers are greatly affected by the PCR reaction system and species level
[0004] At present, there is no report on the use of SRAP molecular markers to analyze the genetic relationship of Moringa strains

Method used

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  • Reaction system and kit for analyzing moringa oleifera genetic relationship and application method thereof
  • Reaction system and kit for analyzing moringa oleifera genetic relationship and application method thereof
  • Reaction system and kit for analyzing moringa oleifera genetic relationship and application method thereof

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Embodiment Construction

[0024] In order to further illustrate the present invention rather than limit the present invention, the following examples will be used for illustration. The experimental methods described in the following examples are conventional methods unless otherwise specified. Mg used in the present invention 2+ , dNTPs, Taq DNA polymerase and DNA Marker were purchased from Dalian Bao Biology Co., Ltd.

[0025] The embodiment of the present invention takes 16 parts of Moringa oleifera strains collected by Fujian Institute of Tropical Crops Science as experimental materials (see Table 1).

[0026] Table 1 Numbers and related information of 16 Moringa oleifera strains

[0027]

[0028]

[0029] The modified CTAB method was used to extract the DNA of 18 Moringa strains, and the specific steps were as follows:

[0030] (1) Take fresh young leaves, add liquid nitrogen to grind them into powder, transfer them to a 2ml centrifuge tube, add 0.9ml preheated 2*CTAB extract and 0.1ml abs...

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Abstract

The invention provides a reaction system and kit for analyzing a moringa oleifera genetic relationship and application thereof. According to the technical scheme provided by the invention, 13 pairs ofprimers with good polymorphism and high definition are screened from 170 pairs of primers through an SRAP (Sequence Related Amplified Polymorphism) molecular marker technology and 18 parts of moringaoleifera DNAs (Deoxyribonucleic Acid) are marked; NTSYS software is used for finishing data analysis and UPGMA (Unweighted Pair-group Method with Arithmetic means) clustering; furthermore, the moringa oleifera genetic relationship and the genetic diversity are researched. A result shows that different moringa oleifera lines have abundant genetic diversity and an SRAP molecular marker is an effective means for analyzing themoringa oleifera germplasm genetic relationship; important technical supports are provided for moringa oleifera molecular assisted breeding and establishment of a genetic map database.

Description

technical field [0001] The invention belongs to the field of biotechnology research, and in particular relates to a kit for analyzing the genetic relationship of Moringa oleifera strains and an application thereof. Background technique [0002] Moringa (Moringa oleifera) is a plant of the genus Moringa in the Moringaceae family. It is a perennial tropical evergreen tree native to India and Africa. It has unique economic value and nutritional health effects. All parts of Moringa can be eaten or used as medicine, and it is widely planted in many developing countries because of its high protein, vitamin and mineral content. There are about 13 species of moringa in the world. With people’s recognition of the efficacy and nutritional value of moringa, in recent years, it has been rapidly promoted and planted in Yunnan, Hainan, Sichuan, Guangdong, Guizhou and Fujian in my country, but some places Blind planting and confusion of species names occur from time to time. [0003] With...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6858C12Q1/6895C12N15/11
CPCC12Q1/6858C12Q1/6895C12Q2600/156C12Q2531/113C12Q2565/125
Inventor 林艺华林宗铿郑涛蔡坤秀
Owner FUJIAN INST OF TROPICAL CROPS
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