Application of mir1432 in rice drought stress

A technology of purpose and drought resistance, applied in the application field of miR1432 in rice drought stress, can solve problems such as unseen research reports

Active Publication Date: 2018-05-04
INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But so far, except for tomato, there are no related research reports on other plants.

Method used

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  • Application of mir1432 in rice drought stress
  • Application of mir1432 in rice drought stress
  • Application of mir1432 in rice drought stress

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Example 1, functional verification of miR1432

[0038] 1. Identification of miR1432 target regulatory genes

[0039] The microRNA target gene prediction tool in PsRobot (http: / / omicslab.genetics.ac.cn / psRobot / ) will be used to predict the target gene of miR1432, and then use Ambion's RLM-RACE kit (AM1700) for 5'RACE, The target genes of miR1432 were further verified.

[0040] MiRNA usually needs to participate in biological processes through its regulation of target genes. In order to study the function of miR1432, its target genes were first predicted by the software psRobot, and then verified by RLM-5'RACE experiments. A class of calcium pump genes (three ACA genes can Targeted and regulated by miR1432, they are LOC_Os02g08010, LOC_Os04g51610 and LOC_Os08g40530. The specific fragment sequencing obtained by RLM-5'RACE shows that the cleavage position of target gene mRNA mediated by miR1432 is as follows figure 1 shown.

[0041] 2. Acquisition of miR1432 gene

[004...

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Abstract

The invention discloses application of miR1432 to rice drought stress and provides application of miR1432 or encoding genes thereof or recombinant vectors containing the encoding genes or expression cassettes containing the encoding genes or transgenic cell lines containing the encoding genes or recombinant bacteria containing the encoding genes or recombinant viruses containing the encoding genes to adjusting and controlling plant drought resistance. The nucleotide sequence of miR1432 is the sequence 1 in a sequence table. Experimental results show that transgenic rice is obtained by interfering in the expression of miR1432 in wild type rice, and compared with non-interference wild type rice, the transgenic rice resists drought stress. Thus, miR1432 can be used for rice high-drought-resistance breeding, and the genetic diversity of existing rice breeding materials is enriched.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to the application of miR1432 in rice drought stress. Background technique [0002] Ca 2+ It occupies a central position in plant cell signal transduction and is an important intracellular signaling molecule, which plays an important role in plant growth and development and stress response. When plants are subjected to different stresses, most of them need to mobilize different physiological processes by changing the concentration of intracellular calcium ions to cope with the adversity. The intracellular calcium ion concentration [Ca 2+ ] cyt The change is mainly achieved by the calcium ion transport system on the membrane, the transport system for [Ca 2+ ] cyt balance plays a key role. Recently, studies have pointed out that some miRNAs in animals and tomatoes can target and regulate calcium ion transport genes on the membrane, and affect the growth and development of animals an...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/113C12N15/82C12N7/01C12N1/21A01H5/00A01H6/46
Inventor 王秀杰王猛储成才方军曹守云
Owner INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI
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