Marker-assisted selection quick incubation method of rice strain containing amylase of middle content

An amylose content, molecular marker-assisted technology, applied in the field of rice lines, can solve the problems of low amplification yield and efficiency, inability to use Wx genotype markers, poor breeding selection guidance effect, etc. The effect of high efficiency, convenient observation and selection, and low cost

Inactive Publication Date: 2009-07-29
CROP INST SICHUAN PROVINCE ACAD OF AGRI SCI
View PDF0 Cites 9 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the existing molecular markers have too low amplified yield and efficiency to effectively mark the Wx genotype, and the guiding effect for breeding selection is poor, and breeding can only be carried out by traditional breeding methods.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] In this example, the maintainer line Chuanxiang 29B of the "three-line" hybrid rice was used as the recipient parent, and the K81 rice line was used as the donor parent to cultivate a rice line with a medium amylose content.

[0028] A kind of molecular marker-assisted selection method of cultivating rice strains with medium amylose content in this example, the practice is:

[0029] A. Using the K81 rice line as the donor parent (male parent) and the maintainer line Chuanxiang 29B (CT repeat number 11) of the "three-line" hybrid rice as the recipient parent (female parent) for hybridization to obtain hybrid F 1 generation population; both donor and recipient parents are also grown;

[0030] to F 1 Molecular marker-assisted selection was performed on individual plants of the generation population, that is, the molecular marker RA19 was used to select F 1 The genomic DNA of the generation single plant and the donor parent was amplified by polymerase chain reaction PCR, ...

Embodiment 2

[0038] In this example, the IR58025B rice line cultivated by International Rice was used as the recipient parent (female parent), and the K81 rice line was used as the donor parent (male parent) to cultivate a rice line with a medium amylose content.

[0039] A, take K81 rice line as donor parent (male parent), and take IR58025B as (CT repeat number is 17) recipient parent (female parent) to carry out hybridization, obtain hybrid F 1 generation population; both donor and recipient parents are also grown;

[0040] to F 1 Molecular marker-assisted selection was performed on individual plants of the generation population, that is, the molecular marker RA19 was used to select F 1 The genomic DNA of the generation single plant and the donor parent was amplified by polymerase chain reaction PCR, and the amplified product was detected by electrophoresis on a 6% polyacrylamide gel; 1 generation single plant;

[0041] B. The donor parent and F selected in step A 1 The generation si...

Embodiment 3

[0048] In this example, the rice line Bo IIIB bred by the Bobai County Agricultural Science Research Institute in Guangxi Province was used as the recipient parent, and the K81 rice line was used as the donor parent to cultivate a rice line with a medium amylose content.

[0049] A kind of molecular marker-assisted selection method of cultivating rice strains with medium amylose content in this example, the practice is:

[0050] A, using the K81 rice line as the donor parent (male parent), and using the maintainer line Bo IIIB (CT repeat number of 18) of the "three-line" hybrid rice as the recipient parent (female parent) to carry out hybridization to obtain a hybrid F 1 generation population; both donor and recipient parents are also grown;

[0051] to F 1 Molecular marker-assisted selection was performed on individual plants of the generation population, that is, the molecular marker RA19 was used to select F 1The genomic DNA of the generation single plant and the donor pa...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to a method for culturing a rice strain with the medium rice amylose content through the molecule label auxiliary selection. The method comprises the following steps: taking a K81 rice strain as a donor parent strain, taking a rice breed or strain with excellent agronomic characters and non-medium amylose content as a receptor parent strain, and carrying out the hybridization of the strains; after the hybridization, carrying out 2 to 3 times of backcross and then carrying out 3 to 5 times of inbreeding; and simultaneously planting the donor parent strain and the receptor parent strain for every generation. During the selection of the generations, a molecule label RA19 is used for auxiliary selection; a current generation individual strain containing a Wx gene of the donor parent strain is selected as the individual strain for the next backcross or inbreeding; and in addition, during the second selection and the later selection of inbred plant, the amylose content is determined for the selection. The method can be used to rapidly and effectively select the rice material with the medium amylose content, reduce the breeding scale and the breeding cost, improve the rice amylose content with high efficiency, and rapidly culture the rice strain with the medium rice amylose content.

Description

technical field [0001] The invention belongs to the technical field of rice molecular breeding, and in particular relates to a method for selecting and cultivating rice strains with medium amylose content by means of molecular markers. Background technique [0002] Rice is the staple food of our people and the main source of food for more than half of the world's population. With the improvement of people's living standards, people have put forward higher requirements for the quality of rice, especially the quality of food. Amylose content is one of the key factors affecting the eating quality of rice. If the amylose content of the rice is too high, the rice will be hard, the rice grains will be loose, and the taste will be poor; otherwise, the rice will be too soft and the palatability will be poor. Although there are differences in the indicators of high-quality rice in various rice-producing countries in the world, most consumers prefer rice with moderate hardness and go...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/04A01H1/02C12Q1/68
Inventor 任光俊李治华曾礼华高方远陆贤军刘光春
Owner CROP INST SICHUAN PROVINCE ACAD OF AGRI SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap