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A method for rapid breeding of rice lines with medium amylose content by molecular marker-assisted selection

A technology assisted by amylose content and molecular markers, applied in the field of rice strains, can solve the problems of inability to mark Wx genotypes, low amplification yield and efficiency, poor breeding selection guidance effect, etc., to achieve convenient observation and selection, Amplification yield and efficiency are high, and the effect of low cost

Inactive Publication Date: 2011-11-30
CROP INST SICHUAN PROVINCE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
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  • Application Information

AI Technical Summary

Problems solved by technology

However, the existing molecular markers have too low amplified yield and efficiency to effectively mark the Wx genotype, and the guiding effect for breeding selection is poor, and breeding can only be carried out by traditional breeding methods.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] In this example, the maintainer line Chuanxiang 29B of the "three-line" hybrid rice was used as the recipient parent, and the K81 rice line was used as the donor parent to cultivate a rice line with a medium amylose content.

[0028] A kind of molecular marker-assisted selection method of cultivating rice strains with medium amylose content in this example, the practice is:

[0029] A. Using the K81 rice line as the donor parent (male parent) and the maintainer line Chuanxiang 29B (CT repeat number 11) of the "three-line" hybrid rice as the recipient parent (female parent) for hybridization to obtain hybrid F 1 generation population; both donor and recipient parents are also grown;

[0030] to F 1 Molecular marker-assisted selection was performed on individual plants of the generation population, that is, the molecular marker RA19 was used to select F 1 The genomic DNA of the generation single plant and the donor parent was amplified by polymerase chain reaction PCR, ...

Embodiment 2

[0038] In this example, the IR58025B rice line cultivated by International Rice was used as the recipient parent (female parent), and the K81 rice line was used as the donor parent (male parent) to cultivate a rice line with a medium amylose content.

[0039] A, take K81 rice line as donor parent (male parent), and take IR58025B as (CT repeat number is 17) recipient parent (female parent) to carry out hybridization, obtain hybrid F 1 generation population; both donor and recipient parents are also grown;

[0040] to F 1 Molecular marker-assisted selection was performed on individual plants of the generation population, that is, the molecular marker RA19 was used to select F 1 The genomic DNA of the generation single plant and the donor parent was amplified by polymerase chain reaction PCR, and the amplified product was detected by electrophoresis on a 6% polyacrylamide gel; 1 generation single plant;

[0041] B. The donor parent and F selected in step A 1 The generation si...

Embodiment 3

[0048] In this example, the rice line Bo IIIB bred by the Bobai County Agricultural Science Research Institute in Guangxi Province was used as the recipient parent, and the K81 rice line was used as the donor parent to cultivate a rice line with a medium amylose content.

[0049] A kind of molecular marker-assisted selection method of cultivating rice strains with medium amylose content in this example, the practice is:

[0050] A, using the K81 rice line as the donor parent (male parent), and using the maintainer line Bo IIIB (CT repeat number of 18) of the "three-line" hybrid rice as the recipient parent (female parent) to carry out hybridization to obtain a hybrid F 1 generation population; both donor and recipient parents are also grown;

[0051] to F 1 Molecular marker-assisted selection was performed on individual plants of the generation population, that is, the molecular marker RA19 was used to select F 1The genomic DNA of the generation single plant and the donor pa...

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Abstract

A molecular marker-assisted selection method for cultivating a rice line with a medium amylose content. The method is: use the K81 rice line as a donor parent, and use a rice variety or line with excellent agronomic properties but not a medium amylose content as a recipient. After crossing, carry out 2-3 times of backcrossing, and then carry out 3-5 times of self-crossing; at the same time, the donor parent and recipient parent are planted in each generation. In each generation of selection, the molecular marker RA19 was used for assisted selection, and the contemporary individual plants containing the Wx genotype of the donor parent were selected as the individual plants of the next backcross or self-cross, and the second and subsequent self-cross plants In the selection, the amylose content was also determined for selection. The method can quickly and effectively select rice materials with medium amylose content, reduce breeding scale, reduce breeding cost, efficiently improve rice amylose content, and rapidly cultivate rice lines with medium rice amylose content.

Description

technical field [0001] The invention belongs to the technical field of rice molecular breeding, and in particular relates to a method for selecting and cultivating rice strains with medium amylose content by means of molecular markers. Background technique [0002] Rice is the staple food of our people and the main source of food for more than half of the world's population. With the improvement of people's living standards, people have put forward higher requirements for the quality of rice, especially the quality of food. Amylose content is one of the key factors affecting the eating quality of rice. If the amylose content of the rice is too high, the rice will be hard, the rice grains will be loose, and the taste will be poor; otherwise, the rice will be too soft and the palatability will be poor. Although there are differences in the indicators of high-quality rice in various rice-producing countries in the world, most consumers prefer rice with moderate hardness and go...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68A01H1/04A01H1/02
Inventor 任光俊李治华曾礼华高方远陆贤军刘光春
Owner CROP INST SICHUAN PROVINCE ACAD OF AGRI SCI
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