Method for performing cross breeding of cymbidium by using molecular marker

Abstract

The invention relates to a method for performing cross breeding of cymbidium by using a molecular marker and belongs to the technical field of biological breeding. The method is characterized by comprising the following cross breeding steps of: extracting a deoxyribonucleic acid (DNA) genome from the cymbidium by a cetyltrimrthyl ammonium-bromide (CTAB) method; screening a primer; selecting a cross parent; breeding the selected parent for blossoming; sampling parent flower powder to perform artificial pollination; and breeding the pollinated parent until a bred fruit pod is ripe. The method has the advantages of creating a new path for the manually selected breeding of a cymbidium variety, making selection from wild plants unnecessary, greatly improving parent selection efficiency, accelerating breeding process and promoting the innovation of a cymbidium germplasm resource. An inter-simple sequence repeat (ISSR) marking technique of the invention has the characteristics of high polymorphism level, simple operation, high repeatability, high stability and the like, can detect rich genetic diversity and select the cross parent which has an appropriate genetic distance and an excellent phenotype in a relatively visualized way in a molecular level so as to increase the success rate of crossing.

Description

Technical field [0001] It belongs to the field of biological breeding technology, and specifically relates to a method for hybrid breeding of national orchids using molecular markers. Background technique [0002] Cymbidium is one of the first genera introduced and cultivated in Orchidaceae plants. National orchid narrowly refers to five ground species in the genus: Spring Orchid (C.goeringii), Autumn Orchid (C.ensifolium), Winter Orchid (C.hanran), Dark Orchid (C.sinense) ) And Whelan Fragrant Orchid (C.faberi), its fragrance is rich, the color is light and elegant, the flowers are beautiful, and the leaves are elegant. [0003] my country has very rich native species and cultivated varieties of National Orchid, and its germplasm resources are quite rich, and it contains extremely rich fine genes, but it has not been developed and utilized in the cultivation of new varieties. At present, the research on Guolan mainly focuses on rapid propagation. There are many literatures on th...

AI Technical Summary

Problems solved by technology

At present, the cultivation of orchid varieties still mainly relies on the traditional concept and practice of selecting from wild plants. The artificial new varieties of orchids have not been given due attention, and the artificially cultivated new varieties with economic value are few and far between. In the cultivation of new varieties of flowers (including other crops in agriculture, forestry and gardening), there is very little practice of selecting varieties from wild variations like Guolan, and the road to the cultivation of new varieties of Guolan should be broad