Method for preparing pure shellfish line

A technology of shellfish and scallop, which is applied in the field of pure line preparation of shellfish, can solve the problems that the pure line of bay scallop has not been seen, difficult gynogenesis preparation, and technical difficulty, so as to reduce research cost, easy popularization and application, and operation easy effect

Inactive Publication Date: 2003-12-17
INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although gynogenetic technology can theoretically establish pure lines faster, it is difficult to establish cultured lines that are suitable for different environments and have obvious trait advantages in a short period of time due to the technical difficulty
What's more, bay scallops are theoretically difficult to carry out effective gynogenetic preparation
So far, there has not been any report on the study of pure lines of bay scallops, nor has there been any report on the establishment of pure lines by inbreeding or self-breeding methods in other shellfish

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Take 100 species of bay scallop (Argopecten irradians irradians) that were originally distributed in the coastal waters of the Atlantic Ocean in the United States and are now cultured in the Chinese sea area, and use the biological characteristics of the hermaphrodite of the bay scallop to establish a pure line through selfing. The operation steps are as follows: :

[0019] 1) Breeding shellfish to promote ripening: put bay scallops with a shell height of 5cm in water at 18°C, with a density of 200 scallops / m 3 , fed with golden algae, diatoms, and chlorella bait, the feeding amount was 180,000 cells / ml per day, and the feeding was divided into 5 times, and the water was changed once a day, and the natural air was filled to supplement the lack of oxygen. It is advisable to see microbubbles on the surface of the water body and form a water flow. Control the light to 400Lux and the effective accumulated temperature to 250°C. At this time, the black film on the surface of ...

Embodiment 2

[0038] The difference from Example 1 is that the seed shells of 16 hermaphroditic bay scallops (Argopecten irradians irradians) that were originally distributed in the Atlantic coastal waters of the United States and are now cultured in the Chinese sea area were successfully selfed, and the biological characteristics of the hermaphroditic bay scallops were utilized. , to establish a pure line through selfing, the operation steps are:

[0039] 1) Seed shellfish to promote ripening: put shellfish with a shell height of 6cm in water at 23°C with a density of 100 shellfish / m 3 , fed with flat algae, diatoms, and Pyramis bait, the amount of feeding was 300,000 cells / ml per day, and the feeding was divided into 6 times. The water was changed once a day, and the natural air was filled to supplement the lack of oxygen. It is advisable to see microbubbles on the surface of the water body and form water flow, control the light to 500Lux, and the effective accumulated temperature to 150°...

Embodiment 3

[0092] The difference from Example 1 is that the implementation steps are:

[0093] 1) Breeding shellfish to promote ripening: place bay scallops with a shell height of 6cm in water at 20°C with a density of 150 shells / m 3 , fed with Pyramiasis, Chlorella, and Flatweed bait, the amount of feeding was 220,000 cells / ml per day, feeding was divided into 5 times, the water was changed once a day, and natural air was filled to supplement the lack of oxygen. It is advisable to see microbubbles on the surface of the water body and form water flow, control the light to 450Lux, and the effective accumulated temperature to 200°C;

[0094] 2) Artificially induced ovulation and ovulation: the individuals that have been artificially stimulated to reach sexual maturity are dried in the shade at 19°C for 1.5 hours, and then each individual is placed in a 1.5L container, and the temperature is raised to 24°C. The seawater is treated with 500mwh / L of ultraviolet rays, and the male and female ...

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PUM

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Abstract

A method for preparing pure shellfish line includes such steps as choosing shellfish by shell height of 5-6 cm, putting them in water at 18-23 deg.C, feeding 5-6 times per day, changing all the water once every day, aerating with air up to visible microbuttle, controlling light irradiation by 400-500 Lux, effective accumulative temp. at 150-250 deg.C, artificially hastening parturition, drying sexually motured shellfish in air at 18-20 deg.C for 1-2 hr, putting in seawater at 24-25 deg.C, ultraviolet radiating by 300-800 mwh/L, obtaining female and male gametes in 0.5-1.5 hr, and self-fertilization.

Description

technical field [0001] The invention relates to shellfish seed production technology, in particular to a shellfish pure line preparation method. Background technique [0002] Bay scallop Argopecten irradians irradians is native to the Atlantic coast of the United States, the left and right shells are almost equal, the shell surface has 18-20 radial ribs, the suitable temperature is 2°C-34°C, and the suitable salt is 25-34°C. It feeds on phytoplankton and organic debris. . The breeding season in the original place is September to October. After being introduced to my country, it can be sexually mature twice in May to June and September to October. The seedlings can reach more than 5cm in the autumn of that year, and the larger individuals can reach 7cm. The smallest biological size is 2.2cm, reaching sexual maturity in that year, and forming a reproductive peak in the next year. The life span is 12 to 16 months, and a few can reach 24 months. [0003] The bay scallop is h...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K61/00
CPCY02A40/81
Inventor 张国范阙华勇刘晓张福绥
Owner INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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