Construction method and application of male sterility line of Argopecten irradians concentricus and Argopecten purpuratus hybridization scallops

Abstract

The invention relates to a construction method and application of a male sterility line of Argopecten irradians concentricus and Argopecten purpuratus hybridization scallops. The male sterility line is a hybridization scallop strain according to which functional sperms cannot be generated, eggs of the male sterility line can be fertilized and grow normally and a male sterility characteristic of the male sterility line can be entailed on offsprings. The Argopecten irradians concentricus and Argopecten purpuratus hybridization scallops are a filial generation between Argopecten irradians concentricus eggs and Argopecten purpuratus sperms. The method comprises the steps that first a large number of first filial generation families obtained after hybridization between Argopecten irradians concentricus and Argopecten purpuratus are constructed, and male sterile individuals are found among the first filial generation families obtained after hybridization between Argopecten irradians concentricus and Argopecten purpuratus; second, sperms of male parent Argopecten purpuratus of the male sterile individuals are used for continuous backcross for seed selection till backcross offsprings are all male and sterile and have fertile eggs, and the male sterility line of the Argopecten irradians concentricus and Argopecten purpuratus hybridization scallops is obtained; third, male sterility line parent scallops are produced on a large scale through hybridization between the male sterility line and a maintainer line, and Argopecten irradians concentricus and Argopecten purpuratus hybridization scallop commercial offspring seeds are produced on a large scale through hybridization between the male sterility line parent scallops and a restorer line. According to the construction method and application of the male sterility line of the Argopecten irradians concentricus and Argopecten purpuratus hybridization scallops, the male sterility line of scallops is constructed among animals for the first time, and the obstacle in self-fertilization of hybridization of hermaphrodite scallops is effectively removed.

Description

technical field [0001] The invention belongs to shellfish breeding technology-a method for cultivating commercial hybrid scallop seedlings, in particular to a construction method and application of a male sterile line of Mozi hybrid scallop (the hybrid offspring of Gulf of Mexico scallop ova and purple scallop sperm). Background technique [0002] The Gulf of Mexico scallop (Argopecten irradians concentricus) is native to the coast of the Gulf of Mexico in the United States and was introduced to my country in the 1990s. The Gulf of Mexico scallop grows fast and adapts to a wide range of temperatures. It is especially suitable for high-temperature sea areas and has strong stress resistance. It is very suitable for farming in the southern seas of my country. It is currently the main scallop cultured in the southern seas. However, due to the long-term inbreeding after the introduction, the germplasm of the Gulf of Mexico scallop has been seriously degraded during the breeding p...

AI Technical Summary

Problems solved by technology

[0006] However, in animal breeding, there have been no reports of male sterile and female fertile individuals at home and abroad so far, so it is impossible to establish male sterile lines, maintainer lines and restorer lines similar to crops for hybrid breeding