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Construction method and application of Zihai hybrid scallop maintainer line

A method for constructing a maintainer line, which is applied in the field of shellfish breeding technology--cultivation of commercial seedlings of hybrid scallops, can solve the problems of inability to establish a maintainer line and a restorer line of male sterile lines, and achieve the effect of overcoming the obstacles of self-fertilization

Active Publication Date: 2016-02-10
烟台海之春水产种业科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] However, in animal breeding, there have been no reports of male sterile and female fertile individuals at home and abroad so far, so it is impossible to establish male sterile lines similar to crops and their maintainer and restorer lines for hybrid breeding

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] 1) Screening of male sterile individuals of Zihai hybrid scallops and breeding of their male parents: 50 purple scallops with mature gonads and 50 bay scallops were selected in the first year, and each scallop was placed in the pre-added scallop after 30 minutes of stimulation in the shade. Spawn eggs and ejaculate in a separate container in seawater at 23°C, collect the eggs of each purple scallop and the sperm and eggs of each bay scallop separately, add a small amount of eggs from a single bay scallop to the egg suspension of each purple scallop Stir to mix the sperm and eggs evenly, observe under a microscope so that there are 5-6 sperm around each egg, and place the fertilized eggs in a dark environment to incubate to obtain the first-generation family of Zihai hybrids. Use the above method to establish at least 20 such hybrid families, and at the same time, mix and fertilize the fertilized bay scallop sperm eggs to establish a corresponding number of bay scallop se...

Embodiment 2

[0025] At the end of March 2008, purple scallops with mature gonads were taken, and purple scallop eggs were obtained after temperature stimulation. After insemination with the sperm of bay scallops, the first-generation seedlings of purple scallops and bay scallops were obtained and raised. In the spring of 2009, the first-generation scallops of the above-mentioned hybrids were matured, and the scallops that were large and only developed in the female area were selected from them. After drying in the shade for 30 minutes, the scallops were placed in a container filled with seawater at 23°C to stimulate spawning. The sperm of scallops were mixed and fertilized, and a backcross population of Zihai hybrid scallops and bay scallops was bred. Most of the backcross populations were hermaphroditic after maturation in the spring of 2010, but <10% of the individuals were still male sterile.

[0026] Continue to backcross the above-mentioned male sterile individuals with the sperm of t...

Embodiment 3

[0028] In the spring of 2010, male sterile individuals from the first generation of hybrids of purple scallop eggs and bay scallop sperm established in 2009 were selected, stimulated to spawn after maturation, and fertilized with bay scallop sperm. 12 backcross families were established and raised. become. After maturation in the spring of 2011, it was found that most of the individuals in 11 of the families were hermaphroditic, while most of the individuals in one of the families only developed the female region, and a small number of individuals were agnostic.

[0029] According to this method, the eggs of a single male sterile individual were backcrossed with the sperm of a single bay scallop to establish several backcrossing families, and the sperm donor bay scallop was selfed to establish corresponding selfing families. In the second year, check the gonad development of the backcross family. If there are male sterile individuals in the backcross family, use the sperm of t...

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Abstract

The invention relates to a purple scallop ovum and bay scallop sperm hybrid scallop maintainer line construction method and application. According to a maintainer line, a corresponding purple scallop ovum and bay scallop sperm hybrid scallop male sterility line offspring is a bay scallop strain which is in male sterility and ovum fertility, and the purple scallop ovum and bay scallop sperm hybrid scallop is the hybrid offspring of the purple scallop ovum and the bay scallop sperm. The purple scallop ovum and bay scallop sperm hybrid scallop maintainer line construction method comprises the following steps of firstly, constructing a great quantity of first filial generation families of the purple scallop ovum and bay scallop sperm hybrid scallop, and finding a male sterility individual for breeding a male sterility line from the first filial generation families; then, continuously backcrossing by the male parent bay scallop sperm to respectively breed a male sterility line-maintainer line combination of which the offspring is completely the male sterility individual; finally, hybridizing to produce male sterility line parent scallop by the male sterility line and the maintainer line, wherein the male sterility line parent scallop is used for producing the offspring seed of the purple scallop ovum and bay scallop sperm hybrid scallop product. According to the invention, the scallop maintainer line is established in an animal for the first time, and therefore, autofertilization obstacles in hermaphroditic scallop hybrid are effectively overcome.

Description

technical field [0001] The invention belongs to shellfish breeding technology-a method for cultivating commercial hybrid scallop seedlings, and in particular relates to the construction and application of a maintainer line of Zihai hybrid scallop (hybrid offspring of purple scallop ova and bay scallop sperm). Background technique [0002] Bay scallops (Argopecten irradians sirradians) are native to the Atlantic coast of the United States and were introduced into my country in 1982. The bay scallop grows fast, has a wide range of temperature adaptability, and has strong stress resistance. It is very suitable for farming in the northern seas of my country. At present, the output and farming area account for more than 50% of the scallop farming in my country. However, due to the long-term inbreeding after the introduction, the germplasm of bay scallops has been seriously degraded during the breeding process. The size of commercial shellfish is getting smaller and smaller, and t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K61/00
CPCY02A40/81
Inventor 王春德孙奎府马斌
Owner 烟台海之春水产种业科技有限公司
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