Construction method and application of Zimo hybrid scallop male sterile line

Abstract

The invention relates to a construction method and application of the male sterility line of hybrid scallops of purple scallops and Argopecten irradians concentricus. The male sterility line cannot generate functional sperms, ova of the male sterility line can be normally fertilized and develop, the male sterility characteristics can be inherited to the descendant hybrid scallop strains, and the hybrid scallops of the purple scallops and the Argopecten irradians concentricus are filial generations of purple scallop ova and Argopecten irradians concentricus sperms. Firstly, a large number of purple scallop and Argopecten irradians concentricus hybrid families of the first filial generation are constructed, male sterile individuals are found out from the purple scallop and Argopecten irradians concentricus hybrid families of the first filial generation, then sperms of male parent Argopecten irradians concentricus of the male sterile individuals are used for continuous backcross breeding until males of all backcross generations are sterile and ova of all the backcross generations are fertile, and the male sterility line of the hybrid scallops of the purple scallops and the Argopecten irradians concentricus can be obtained; the male sterility line and a maintainer line are used for hybridization to produce parent scallops of the male sterility line in a large scale, and the parent scallops of the male sterility line and a restorer line are used for hybridization to produce commodity offspring seeds of the hybrid scallops of the purple scallops and the Argopecten irradians in a large scale. According to the construction method and application, the male sterility line of the scallops is firstly built in animals, and the obstacles of autofertilization of hermaphrodite scallops in the hybridization process are effectively eliminated.

Description

technical field [0001] The invention belongs to shellfish breeding technology-a method for cultivating commercial hybrid scallop seedlings, and in particular relates to a construction method and application of a male sterile line of Zimo hybrid scallop (the hybrid offspring of purple scallop ova and Mexican gulf scallop sperm). Background technique [0002] The Gulf of Mexico scallop (Argopecten irradians concentricus) is native to the coast of the Gulf of Mexico in the United States and was introduced to my country in the 1990s. The Gulf of Mexico scallop grows fast and adapts to a wide range of temperatures. It is especially suitable for high-temperature sea areas and has strong stress resistance. It is very suitable for farming in the southern seas of my country. It is currently one of the main scallop breeding species in the southern seas. However, due to the long-term inbreeding after the introduction, the germplasm of the Gulf of Mexico scallop has been seriously degra...

AI Technical Summary

Problems solved by technology

[0007] The purpose of the present invention is to realize in animal breeding, specifically to establish the construction method of Zimo hybrid scallop male sterile line for the first time in hermaphroditic scallop, to overcome the deficiency of existing hybrid scallop breeding technology