39 results about "Interspecific hybrids" patented technology

The invention relates to a male gamete killer gene S19g-A1 of a rice interspecific hybrid sterile S19 seat and application of the male gamete killer gene S19g-A1. The invention provides a rice hybrid sterility related protein S19g-A1. The amino acid sequence of the rice hybrid sterility related protein S19g-A1 is as shown in SEQ ID NO. 2. According to the invention, a key gene S19g-A1 of a rice interspecific hybrid sterile S19 site is researched and identified, function knockout is carried out on African rice S19g-A1, and pollen fertility of a mutant hybrid F1 obtained by carrying out distant hybridization on an obtained African rice s19g-a1 mutant returns to normal. Based on a gene editing technology, the invention develops a method for rapidly creating a hybrid affinity line capable of overcoming sterility of African rice hybrid, effectively eliminates S19 locus mediated interspecific hybrid reproductive disorder, breaks through the bottleneck of rice interspecific hybrid advantage utilization, provides target gene resources for distant hybrid advantage utilization and crop yield improvement, and has a wide application prospect. Good application prospects are realized.

The present invention discloses a molecular marker for identifying Vitis vinifera and its interspecific hybrid grapes and its application, belonging to the technical field of biological breeding. Through the analysis of whole-genome resequencing data, the present invention identifies a significant InDel locus IH1, which is located at 7,224,357 bp on chromosome 13 of grapes. This locus shows significant genotypic differences between Vitis vinifera grapes and their interspecific hybrids. Through the differences in the number and distribution of electrophoretic bands, Vitis vinifera grapes and their interspecific hybrids can be visually and accurately distinguished. Based on the characteristics of this molecular marker, it can be used for rapid screening of interspecific hybrid offspring lines in the early stage of breeding to eliminate self-crossed offspring. At the same time, it can also rapidly screen the genetic background of seedlings to ensure the accuracy of seedlings, which plays an important role in the early screening of breeding offspring and the screening of the genetic background of seedlings.

The present invention discloses a set of SSR molecular markers for identifying wild chrysanthemum and Shennong fragrant chrysanthemum germplasm resources, consisting of 21 pairs of SSRs numbered SSR01-SSR21. The primer sequences for amplifying these 21 pairs of molecular markers are shown in SEQ ID NO: 1-42, and the present invention belongs to the field of molecular biology technology. Using these 21 pairs of SSR molecular markers, the collected wild chrysanthemum and Shennong fragrant chrysanthemum can be identified and the germplasm genetic diversity analyzed. The present invention uses SSR markers for the first time to jointly detect the genetic diversity of wild chrysanthemum and Shennong fragrant chrysanthemum germplasm. This set of SSR molecular markers has good polymorphism and a high level of genetic diversity. It can be used in the fields of kinship analysis, resource genetic evaluation, identification of interspecific hybrid offspring, positioning of trait genes, and molecular marker-assisted breeding of wild chrysanthemum and Shennong fragrant chrysanthemum.

The invention discloses a method for creating offspring of buckwheat interspecific hybridization. According to the invention, the immature embryo can be successfully obtained through artificial hybridization by taking wild buckwheat as a female parent and tartary buckwheat as a male parent; in order to solve the problems that the induction rate of an immature embryo on a callus induction culture medium is low and the differentiation rate is low when the callus is subjected to differentiation culture in a differentiation culture medium, the callus induction culture medium and the differentiation culture medium are optimized and screened; the obtained callus induction culture medium and the obtained differentiation culture medium remarkably improve the callus induction rate and the differentiation rate during differentiation culture, regenerated plants are obtained at high frequency, colchicine doubling is performed on the regenerated plants on the basis, and a doubled interspecific hybridization new germplasm is obtained by combining morphological and cytological identification. The method is applied to breeding of high-yield stress-resistant, high-flavone, perennial and other breakthrough new varieties or serves as a hybrid bridge parent, and the excellent characters of stress resistance, high flavone, perennial and the like of wild buckwheat are introduced into buckwheat cultivation.

The application relates to the field of molecular breeding, in particular to a molecular marker for identifying interspecific hybrid of brassica rapa and coelonema and application thereof. The molecular marker comprises one pair of brassica rapa specific primers and one pair of coelonema specific primers. The identification method comprises the following steps: obtaining a sample to be identified, taking genomic DNA of the material as a template, and using two specific primer pairs for amplification; performing agarose gel electrophoresis operation on the amplified product; and judging the authenticity of the interspecific hybrid by observing the electrophoresis strip.

The present invention discloses a method for identifying interspecific hybrid germplasm of Rubus rubus, comprising the following steps: using hexaploid cultivated blackberry as the female parent and diploid wild Rubus rubus as the male parent, obtaining hybrid seeds, and cultivating interspecific hybrid seedlings; designing male parent SSR primers, amplifying the DNA of the interspecific hybrid seedlings, and screening primer combinations to identify true hybrids; verifying the amplified bands of the screened SSR primer pairs in wild Rubus rubus, raspberry, and blackberry varieties, and optimizing specific primers for identifying hybrid offspring of different wild Rubus male parents. The screened SSR primers are used to identify blackberry cultivars and interspecific hybrid seedlings of Rubus rubus, and the true hybrids are identified and the parental germplasm sources are confirmed based on the amplified bands. The present invention successfully hybridizes diploid wild Rubus rubus with hexaploid cultivated blackberry varieties for the first time, uses the SSR molecular markers obtained through screening to identify hybrids, and verifies that the wild germplasm has entered the cultivated varieties through hybridization.

The present application relates to cherry molecular marker assisted germplasm identification and breeding technical field, specifically to a kind of InDel marker for identifying Chinese cherry and European sweet cherry germplasm and its application, suitable for identifying cherry resources of Chinese cherry and European sweet cherry two species and interspecific hybrid thereof.The present application designs specific PCR primer using InDel sequence information of cherry genome Cp2_35932912 site, extracts Chinese cherry and European sweet cherry genomic DNA, carries out PCR amplification and polyacrylamide gel electrophoresis, reads electrophoresis band, identifies Chinese cherry, European sweet cherry and interspecific hybrid thereof according to band position and number, develops InDel molecular marker, which can be used for Chinese cherry and European sweet cherry germplasm identification, and also can be used for early identification of interspecific hybridization offspring hybrid authenticity, and the result is accurate and reliable.

The present disclosure relates to Cucurbitaceae allotetraploid plants and hybrid allopolyploid plants having desirable traits, such as resistance to an abiotic or biotic stressor, which may be used as rootstock for cultivated varieties. The disclosure further relates to composite Cucurbitaceae plants comprising the allopoly ploid plants described herein as the rootstock. The disclosure further relates to methods of producing Cucurbitaceae allopolyploid plants and hybrid allopolyploid plants.

The invention relates to a design and use method of a specific probe library for accurately identifying wild species chromosomes in peanut interspecific hybrids, which specifically comprises the following steps: assembling a T2T reference genome of a peanut related wild species Zw61, then carrying out bioinformatics analysis on the genome sequence and published Tifrunner, YZ9102, A. duranensis and A. ipaensis reference genomes, and finally identifying the chromosomes of the wild species in the peanut interspecific hybrids. An oligonucleotide library containing 91500 cultivated species and Zw61 variation group sequences is obtained, the oligonucleotide library comprises 10 oligonucleotide pools corresponding to chromosomes 1-10 of Zw61, the oligonucleotide library can be used for accurately identifying artificially synthesized hexaploid F1 of the cultivated species and the wild species and exogenous chromosomes in hybrid progenies through single-chromosome coating, and the identity of the exogenous chromosomes can be accurately determined; the problem that peanut distant hybridization exogenous chromosomes are difficult to identify is solved, and particularly the identification problem of exogenous chromosomes which are very close to cultivated species in genetic relationship and high in chromosome homology is solved.

The invention relates to the technical field of breeding, in particular to a method for breeding a sterile line of japonica rice with wide affinity, high exposure and early blossoming. According to the method, through combination of multi-parent hybridization and multiple cross with marker-assisted selection and phenotype tracking, accurate polymerization of multiple characters such as wide affinity, high exposure and early blossoming is realized, the breeding efficiency is high, the bred 'Ning46A' is high in seed production suitability, can be matched with an indica-japonica restorer line, fully utilizes the heterosis between subspecies, and has important production and application values.

The invention relates to the technical field of molecular marker-assisted germplasm resource identification and breeding of cherries, in particular to an InDel marker for identifying germplasm of Chinese cherries and European sweet cherries and application of the InDel marker, which are suitable for identifying two species of the Chinese cherries and the European sweet cherries and interspecific hybrid cherry resources of the two species of the Chinese cherries and the European sweet cherries. The InDel molecular marker is developed by designing a specific PCR (Polymerase Chain Reaction) primer by utilizing InDel sequence information of a cherry genome Cp235932912 site, extracting genomic DNA (Deoxyribose Nucleic Acid) of Chinese cherries and European sweet cherries, performing PCR amplification and polyacrylamide gel electrophoresis, reading electrophoretic bands, identifying the Chinese cherries, the European sweet cherries and interspecific hybrids thereof according to the positions and the number of the bands, and identifying the Chinese cherries, the European sweet cherries and the interspecific hybrids of the Chinese cherries, the European sweet cherries and the European sweet cherries according to the InDel molecular marker. The molecular marker can be used for germplasm identification of Chinese cherries and European sweet cherries, and can also be used for early identification of hybrid authenticity of interspecific filial generations of the Chinese cherries and the European sweet cherries, and results are accurate and reliable.

The present invention provides a method for identifying progeny of interspecific hybrids between walnut and Hebei walnut, relating to the technical field of plant genetic breeding. The present invention provides the use of specific primers in identifying progeny of interspecific hybrids between walnut and Hebei walnut, as well as a method for identifying progeny of interspecific hybrids between walnut and Hebei walnut. The method is simple to operate, has good repeatability, high stability, and is unaffected by the external environment. The method effectively improves the authenticity of hybrid offspring between walnut varieties, increases the efficiency of early selection of hybrid offspring, shortens the breeding cycle, and improves breeding efficiency.

The invention relates to the field of biotechnology breeding, in particular to a method for obtaining an interspecific filial generation of elephant trunk orchid and calanthe calyx orchid by using embryo rescue. According to the method, the calanthe ciliata is used as a male parent, the elephant trunk is used as a female parent, the embryo rescue technology is combined, in the embryo rescue technology, every 1 L of embryo rescue culture medium is composed of 2 g / L of Huabao No.1, 100 g / L of potato homogenate, 30 g / L of cane sugar, 0.5 g / L of activated carbon, 7.0 g / L of agar and the balance of water, and the pH is 5.6-5.8. According to the method, germination of the hybrid embryo of the calanthe ciliata and the elephant trunk can be remarkably promoted, new genetic characteristics are introduced potentially, and the hybrid embryo is obtained. The genetic diversity of the phalaenopsis variety is enhanced, and a new germplasm resource is provided for breeding of the phalaenopsis.

The present invention discloses an InDel marker primer for identifying the authenticity of the F1 generation of interspecific hybridization of sisal hemp and its application. According to the resequencing data of sisal parental varieties, the present invention develops an InDel marker primer for identifying the authenticity of the F1 generation of hybridization of sisal hemp '292' and 'Moyin 7', and performs identification at the seedling stage of the hybrid F1 generation. The results show that the offspring that can simultaneously amplify the characteristic bands of the father and the mother are true hybrids, and the lack of any one of the bands or the lack of two bands are regarded as false hybrids, which improves the detection purity of the true hybrids of the F1 generation and also advances the identification time. At the same time, a method for identifying the authenticity of the F1 hybrids of interspecific hybridization of sisal hemp varieties is provided. Compared with the traditional hybrid identification method, it is fast, convenient, accurate and reliable, has a short breeding cycle and low breeding cost, and can also screen the breeding target offspring early, effectively improve the identification efficiency of interspecific hybrids of sisal hemp, and provide a more effective method for quickly identifying the authenticity of the offspring of interspecific hybridization of sisal hemp.

This invention discloses a method for creating a flavorful sweet and sour melon through interspecific hybridization of thick-skinned melon and cucumber. Using the thick-skinned melon inbred line 'Baipicui' as the female parent and the cucumber inbred line 'Jinyou 406' as the male parent, artificial pollination and hybridization are performed to obtain interspecific hybrid offspring. These offspring are then subjected to several generations of self-pollination and selection to obtain the flavorful sweet and sour melon. This invention is the first to utilize interspecific hybridization between high-quality thick-skinned melon and cucumber to create a new high-quality flavorful sweet and sour melon germplasm. This new germplasm has a moderate sweet and sour taste, a well-proportioned fruit shape, and green flesh, and can be directly used for melon variety improvement.

The application provides a specific molecular marker of Malus sieversii non-stinging No.1 non-stinging strain and application thereof, and belongs to the technical field of crop molecular breeding. The application can simply and quickly identify the non-stinging No.1 non-stinging strain in Malus sieversii materials by simultaneously detecting two marker sites through one double-PCR, and using two InDel markers, and meanwhile, the simultaneous detection of the two marker sites can ensure the accuracy, effectiveness and reliability of the detection results by mutual verification and mutual testing, so that the application has great application value in the aspects of distinguishing Malus sieversii varieties and identifying interspecific hybrid seeds, and can provide technical support for innovative large-fruit non-stinging fresh-eating Malus sieversii varieties. In addition, the application can shorten the breeding cycle of non-stinging Malus sieversii and accelerate the breeding process by extracting leaf genomic DNA of the seedling of the breeding material at the seedling stage for double-PCR amplification, and identifying whether it is the non-stinging No.1 non-stinging strain by observing electrophoresis bands.

The present disclosure relates to eggplant and pepper allotetraploid plants and hybrid allopolyploid plants having desirable traits, such as resistance to an abiotic or biotic stressor, which may be used as rootstock for cultivated varieties. The disclosure further relates to composite eggplants and pepper plants comprising the allopolyploid plants described herein as the rootstock. The disclosure further relates to methods of producing eggplant and pepper allopolyploid plants and hybrid allopolyploid plants.

The invention relates to the field of molecular breeding, in particular to a primer for identifying a Chinese cabbage and blue fragrant mustard intergeneric hybrid based on a KASP technology and application of the primer. The identification method comprises the following steps: obtaining a to-be-identified sample, taking genome DNA of the material as a template, and performing amplification by using a KASP primer pair; and carrying out fluorescence signal detection on the amplified product to judge the authenticity of the intergeneric hybrid.

The utility model discloses a melon interspecific hybrid tissue culture seedling domestication device relates to plant seedling domestication device technical field. Its structure includes nutrient solution groove, matrix groove, double -deck light -transmitting light -supplementing cover and ventilation adjustment assembly, and the matrix groove is installed in the nutrient solution groove, and ventilation adjustment assembly is installed on the nutrient solution groove and matrix groove, and ventilation adjustment assembly divides matrix groove and is divided into a plurality of seedling domestication area, and every seedling domestication area installs a double -deck light -transmitting light -supplementing cover. The utility model discloses through double -deck light -transmitting light -supplementing cover and temperature feedback system realizes environment accurate control, adopts M shape cotton line, filter cotton section constructs microcirculation nutrition supply system, and combines the partition formula modular design and promotes space utilization rate and operating efficiency, and the device is verified through the test, can make interspecific hybrid domestication survival rate to improve, and domestication period shortens, provides efficient technical solution for melon interspecific hybrid breeding.

The invention relates to the field of plant conservation and breeding optimization, in particular to a hybrid embryo rescue method and a method for creating a large number of interspecific hybrid seedlings of Populus nigra by using the hybrid embryo rescue method. The method comprises the following steps: establishing an embryo rescue system, namely observing a separation layer between infructescence and branches after pollination for about 2 months, cutting part of infructescence to develop the embryo rescue system, washing capsules with running water for 120 minutes, lightly wiping peels with 75% alcohol for about 2 minutes, washing the capsules with sterile water for 5-6 times in a super clean bench, lightly absorbing moisture on the surfaces of the capsules with sterile filter paper, and taking out the capsules; the method comprises the following steps of: cutting a capsule by using a sterile scalpel, thoroughly removing floc, carefully clamping seeds by using sterile tweezers, putting the seeds on a 1 / 2MS culture medium containing auxin analogues for embryo rescue, and screening out an optimal culture medium which is 1 / 2MS + 0.06 mg / L naphthylacetic acid (NAA) + 0.08 mg / L indolebutyric acid (IBA) and has the pH value of 5.8-6.0 by adopting a culture dish germination method and taking 1 / 2 MS as the culture medium.

This invention discloses a gene locus HS12 for intersubspecies hybrid sterility in rice (indica-japonica) and its applications, belonging to the field of plant genetic engineering. This locus is located at the end of the long arm of chromosome 12, between molecular markers Sy55 and Sy8, and contains four fertility-related genes: iTx1, iTx2, iAd1, and jAd2. By functionally knocking out or suppressing the expression of genes iTx1 and / or iTx2, and then introducing iAd1 and / or jAd2, broadly compatible rice materials can be created. It also provides molecular markers for detecting HS12, which can be used for hybrid progeny detection and assisted breeding, effectively overcoming indica-japonica hybrid sterility and improving the seed setting rate of offspring.

This invention discloses SSR marker primers specific to the filamentous petals of gerberas and their applications. This invention utilizes SSR molecular markers to effectively identify different filamentous petal traits in gerberas, resulting in an SSR marker primer that can rapidly and accurately identify the petal traits of gerbera plants with different filamentous petals and the F1 generation of interspecific hybrids. The SSR marker primers provided by this invention can effectively distinguish between filamentous and non-filamentous petals in gerberas and exhibit significant polymorphism, with an accuracy greater than 65%. Furthermore, compared with traditional morphological trait observation methods, the identification method provided by this invention has better repeatability, saves time, and has high specificity. It can replace traditional morphological identification methods, effectively improving the molecular-level breeding process of gerberas and reducing the identification cycle of traditional morphological trait observation. It also allows for early screening to obtain gerbera offspring with superior filamentous varieties, which is of great significance for gerbera breeding.

The invention is applicable to the technical field of molecular biology, and provides an InDel marker and a primer group for identifying species of sika deer and red deer and application of the InDel marker and the primer group, the InDel marker is located at a 6116106 site of a fifth chromosome NC057819.1 of the red deer, 120bp of a DNA sequence shown in a sequence table SEQ ID NO: 1 is deleted at the site, and the DNA sequence exists in the sika deer. The invention also provides a primer group for identifying species of sika deer and red deer. The primer group comprises an upstream primer and a downstream primer which are designed aiming at the InDel marker. Based on high-throughput whole genome data of the sika deer and the red deer, the interspecific InDel marker is screened, a corresponding genetic typing technology is established, a technical system which is efficient in detection, low in cost and suitable for large-scale sample screening is constructed, accurate identification of the sika deer, the red deer and interspecific hybrid individuals can be achieved, and better application value is achieved.

This invention relates to a male gamete protection gene S19g-A7 and its application in improving rice hybrid fertility and hybrid rice breeding. This invention identifies a male gamete protection gene S19g-A7 at the S19 locus of interspecific hybrid sterility in rice, whose encoded amino acid sequence is shown in SEQ ID NO.2. Introducing the S19g-A7 gene into Asian rice for functional complementation can break the S19 locus-mediated interspecific hybrid sterility in rice, rapidly creating hybrid compatible lines that overcome interspecific hybrid sterility in Asian and African rice. This provides genetic resources and breeding methods for utilizing distant hybrid vigor in high-quality rice germplasm breeding, and has significant application value.

The application relates to a method for identifying Brassica rapa interspecific hybrid allopolyploids based on InDel technology and a primer pair. The method comprises the following steps: obtaining a sample to be identified, wherein the sample is obtained by performing interspecific hybridization with Brassica rapa '72049' as a female parent and Eruca vesicaria 'ASTRO' as a male parent, and then performing embryo rescue and chromosome doubling; taking genomic DNA of the material as a template, and performing amplification by using an InDel primer pair; performing gel electrophoresis on the amplified product; and observing an electrophoresis strip to determine the authenticity of the allopolyploid.

The present disclosure relates to eggplant and pepper allotetraploid plants and hybrid allopolyploid plants having desirable traits, such as resistance to an abiotic or biotic stressor, which may be used as rootstock for cultivated varieties. The disclosure further relates to composite eggplants and pepper plants comprising the allopolyploid plants described herein as the rootstock. The disclosure further relates to methods of producing eggplant and pepper allopolyploid plants and hybrid allopolyploid plants.

The present application belongs to the technical field of preparation of excellent commercial seed of economic marine shellfish, and particularly relates to a high-efficiency method for preparing interspecific three-way hybrid of Argopecten genus scallop. The method uses a selected and purified male sterile and female fertile hybrid Argopecten mactrolineatus as a female parent, and a restocked Argopecten purpuratus as a male parent, to produce a "Argopecten mactrolineatus Argopecten purpuratus" three-way hybrid scallop commercial seed with hybrid vigor and outstanding stress resistance. The method can realize large-scale, continuous and efficient hybrid seed production, and has the advantages of simple operation, labor saving, low dependence on personnel, and the like. The hybridization rate is increased from less than 30% in the prior art to more than 90%, and the efficiency of interspecific hybrid seed production and the purity of the seed of the protandrous Argopecten genus scallop are significantly improved.

This invention discloses a method for interspecific hybridization and seedling cultivation of *Pterocarya spp.* and *Pterocarya martensii*, comprising: selecting mature, live male *Pterocarya spp.*, cleaning and drying them, placing them in seawater, continuously aerating and artificially circulating the water to induce natural sperm release; dissecting mature female *Pterocarya martensii* to extract gonads, collecting mature eggs, cleaning them, and allowing them to settle to obtain an egg mixture; adding ammonia to the egg mixture to activate the eggs; adding the activated egg mixture to a sperm-containing water body for fertilization in the dark; after successful fertilization, removing the male *Pterocarya spp.* and transferring the fertilized eggs to a seedling pond; and cultivating the fertilized eggs in seawater to develop into D-shaped larvae. This invention is the first of its kind for distant hybridization and seedling cultivation of *Pterocarya spp.* and *Pterocarya martensii*, breaking through the reproductive isolation between the two species, achieving stable and efficient hybridization and fertilization. The process is simple and highly stable, and can cultivate new hybrid pearl oyster seedlings that combine the excellent traits of both parents. It has significant implications and promotional value for the innovation of marine pearl oyster germplasm and industrial upgrading.

A molecular marker for identifying Vitis vinifera and its interspecific hybrid grapes and an application thereof are provided. Through whole-genome resequencing data analysis, a significant InDel locus IH1 is identified, which is located at 7,224,357 base pairs (bp) on chromosome 13 of grapes. This locus shows genotypic differences between Vitis vinifera and its interspecific hybrids. Through the differences in the number and distribution of electrophoretic bands, Vitis vinifera and its interspecific hybrids may be distinguished. Based on the characteristics of this molecular marker, it may be used for screening of interspecific hybrid progeny lines in the early stage of breeding and elimination of selfed progeny. At the same time, it may also conduct screening of the genetic background of seedlings.

The invention belongs to the field of identification of interspecific distant hybrids of plants, and particularly relates to a molecular marker for identifying interspecific hybrids of allium fistulosum and allium macrostemon and application of the molecular marker. The molecular marker is an ITS sequence, the ITS sequence is shown as SEQ ID NO.1-2, the ITS sequence is an actual analysis assembly sequence of Zhangzhu allium fistulosum, Allium sibiricum and a hybrid F1-16, a primer group capable of being correctly amplified is designed and verified according to the ITS sequence and then is applied to identification of the Zhangzhu allium fistulosum and Allium sibiricum interspecific hybrid, the detection period is short, the operation is simple and convenient, the detection process is economical and economical, and the method is suitable for large-scale popularization and application of the Zhangzhu allium fistulosum and Allium sibiricum interspecific hybrid. The method is accurate and reliable in identification result and good in repeatability, overcomes the defects that a traditional morphological identification method is inaccurate, time-consuming and labor-consuming, plays a positive role in genetic relationship identification and genetic breeding of the high-quality germplasm resources of the green Chinese onions, and provides an effective method for identification and protection of the germplasm resources.