Identification method of interspecific hybrids of Brassica campestris and Brassica napus
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A technology of Brassica napus and Brassica napus, applied in the field of plant cytology, achieves the effects of simple technology, improved accuracy, and high identification efficiency
Inactive Publication Date: 2012-11-07
GANSU AGRI UNIV
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The leaf guard cell chloroplast counting method is easy to operate, accurate in results, and high in identification efficiency. It has been applied to crops such as potatoes, tobacco, peanuts, and grapes. There are also studies that use the number of chloroplasts as a ploidy dividing index for identifying crops such as mulberry leaves and loquats. Research. But so far there is no report on the use of leaf stomatal guard cell perimeter and chloroplast number to identify the authenticity of rapeseed interspecific hybrids
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Embodiment 1
[0021] Embodiment 1: a kind of method for interspecific hybrid identification of Chinese cabbage-type rape and Brassica napus, it comprises the following steps:
[0022] (1) For the configuration of 1% iodine-potassium iodide staining solution, weigh 1g of iodine and pour it into a small beaker, add 80ml of distilled water to dissolve, then add 2g of potassium iodide; set the volume to 100ml for later use;
[0023] (2) Pick the flat leaves at the sixth leaf position of the interspecific hybrid F1 of Chinese cabbage-type rapeseed Longyou No. 9, Brassica napus vision and the interspecific hybrid F1 of Chinese cabbage-type rapeseed No. 9 and Brassica napus at the seedling stage, and put them into plastic bags , put it in a prepared ice box below 0°C within 5-10 minutes, and bring it back. After the leaves are washed with distilled water, put them in a fume hood to dry the water on the surface of the leaves, cut the lower part of the leaves with a blade, and tear them off with twee...
Embodiment 2
[0046] Example 2: A method for identification of interspecific hybrids between Chinese cabbage-type rape and Brassica napus, in step (2), picking Chinese cabbage-type rape Longyou No. 9, Brassica napus vision and Brassica napus Longyou 9 at the seedling stage No. and Brassica napus vision interspecific hybrid F1 The flattened leaves at the 4th leaf position were packed into plastic bags; the rest were the same as in Example 1.
[0047] Verification example:
[0048] The identification method is as in Example 1. The existing 40 interspecific hybrids of Chinese cabbage-type rape Yanyou 2 and Brassica napus Qinchuan Donggan 1 were identified by the above-mentioned method.
[0049] Experimental steps:
[0050] This 40 seeds are placed in the petri dish, spray a small amount of water, allow it to germinate, when the root grows to about 4mm, move into the nutrient bowl, put the tissue culture room to grow, room temperature is controlled at about 25 ℃. At the seedling stage, the yo...
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Abstract
The invention discloses an identification method of interspecific hybrids of Brassica campestris and Brassica napus, falling into the technical field of plant cytology. The method includes collecting the 4th-7th leaves of a plant in the seedling stage, tearing to collect hypoderm tissues of lower parts of the leaves, staining with iodine-potassium iodide solution, observing stomata under an optical microscope, measuring major/minor axis values of guard cells, calculating perimeter values of the guard cells with an ellipse model while observing and counting chloroplasts, recording 15 data per leaf, and identifying with parents as controls. For Brassica campestris, stoma guard cells have perimeter smaller than 58.90 micrometer and chloroplast count of 9-11; for Brassica napus, stoma guard cells have perimeter greater than 75.83 micrometer and chloroplast count of 19-20; while, for the to-be-identified material, if more than ten stomata simultaneously satisfy that guard cells have perimeter of 58.90-75.83 micrometer and chloroplast count of 14-16, it is determined that the plant is interspecific hybrids F1 of Brassica campestris and Brassica napus, otherwise it is false hybrid.
Description
technical field [0001] The invention belongs to the technical field of plant cytology application, and in particular relates to a method for identifying interspecific hybrids of Chinese cabbage-type rape and Brassica napus. Background technique [0002] Rapeseed is an important oil crop in the world. Variety is the key to the development of rapeseed, and the identification of distant hybrid breeding is an important way to select new varieties. In order to improve the selection efficiency of distant hybrid breeding and true hybrid breeding, timely and accurately identify hybrid F1 Authenticity is of great significance. The traditional method commonly used in the identification of early interspecific hybrids is the identification of morphological markers. Morphological markers refer to crop-specific external characteristics that are visible to the naked eye and simple and intuitive, or certain traits that can be identified with simple tests. It mainly includes botanical trait...
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