Interspecific hybrid embryo rescuing and seedling forming method of sweet cherries in southern China and Chinese cherries
A technology of Chinese cherries and southern regions, applied in horticultural methods, botanical equipment and methods, plant regeneration, etc., can solve problems such as the impact on the success rate of embryo rescue, save sterilization time, reduce the risk of germplasm loss, and simplify assembly. The effect of training steps
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Embodiment 1
[0031]The hybrid embryo rescue technology between sweet cherry and Chinese cherry in southern China includes the following steps:
[0032] A. Pollen collection and pollination: Chinese cherry 'Amethyst' and 'Short Handle' were used as the male parent to collect natural flowers during the flowering period of bells. The anthers were peeled off indoors and dried in the shade at 25°C. After the anthers crack and loose powder, they are collected in small glass bottles, sealed and stored in a refrigerator at 4°C for future use.
[0033] Using the sweet cherry 'Chaoyang No.1' as the female parent, artificial non-emasculation pollination was carried out on the bell stage flowers. Cover sulfuric acid paper bag immediately after pollination and change mesh bag after 7 days.
[0034] B. Fruit picking and low temperature layering treatment: such as figure 1 As shown in C, young hybrid fruits were harvested at the best stage for hybrid embryo rescue (PF >0.5 at the hard nucleus stage)....
Embodiment 2
[0045] The hybrid embryo rescue technology between sweet cherry and Chinese cherry in the southern region comprises the following steps:
[0046] A. Collect pollen and pollinate: Collect the natural flowers of Chinese cherry 'Gejiawu' bell flowering period. The anthers were peeled off indoors and dried in the shade at 25°C. After the anthers cracked and loosened the powder, they were collected in small glass bottles, sealed and stored in a refrigerator at 4°C for future use.
[0047] Using the sweet cherry 'Chaoyang No.1' as the female parent, artificial non-emasculation pollination was carried out on the bell stage flowers. Cover sulfuric acid paper bag immediately after pollination and change mesh bag after 7 days.
[0048] B. Fruit picking and low-temperature stratification treatment: The best time to collect 'Chaoyang No. 1'×'Gejiawu' embryos is 37 days after pollination. Put the fresh fruits in a transparent plastic fresh-keeping box and store them in a 4 ℃ low-temperat...
Embodiment 3
[0059] The hybrid embryo rescue technology between sweet cherry and Chinese cherry in the southern region comprises the following steps:
[0060] A. Collect pollen and pollinate: Collect the natural flowers of Chinese cherry 'Black Pearl' bell flowering period. The anthers were peeled off indoors and dried in the shade at 25°C. After the anthers cracked and loosened the powder, they were collected in small glass bottles, sealed and stored in a refrigerator at 4°C for future use.
[0061] With the sweet cherry 'Chaoyang No. 1' as the female parent, the flowers at the bell stage were artificially pollinated without emasculation. Immediately after pollination, cover the sulfuric acid paper bag and change the mesh bag after 7 days.
[0062] B. Fruit harvesting and low-temperature stratification treatment: The best time to save 'Chaoyang No. 1'×'Black Pearl' embryos is 37 days after pollination. After harvesting, fresh fruits should be placed in transparent plastic fresh-keeping ...
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