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Interspecific hybrid embryo rescuing and seedling forming method of sweet cherries in southern China and Chinese cherries

A technology of Chinese cherries and southern regions, applied in horticultural methods, botanical equipment and methods, plant regeneration, etc., can solve problems such as the impact on the success rate of embryo rescue, save sterilization time, reduce the risk of germplasm loss, and simplify assembly. The effect of training steps

Pending Publication Date: 2019-07-26
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, in the process of embryo rescue, factors such as embryo age, embryo skin, low-temperature stratification method, embryo disinfection method, medium type, carbon source, type and ratio of plant growth regulators, and seedling transplanting methods will all affect the success of embryo rescue. rate of impact

Method used

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  • Interspecific hybrid embryo rescuing and seedling forming method of sweet cherries in southern China and Chinese cherries
  • Interspecific hybrid embryo rescuing and seedling forming method of sweet cherries in southern China and Chinese cherries

Examples

Experimental program
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Effect test

Embodiment 1

[0031]The hybrid embryo rescue technology between sweet cherry and Chinese cherry in southern China includes the following steps:

[0032] A. Pollen collection and pollination: Chinese cherry 'Amethyst' and 'Short Handle' were used as the male parent to collect natural flowers during the flowering period of bells. The anthers were peeled off indoors and dried in the shade at 25°C. After the anthers crack and loose powder, they are collected in small glass bottles, sealed and stored in a refrigerator at 4°C for future use.

[0033] Using the sweet cherry 'Chaoyang No.1' as the female parent, artificial non-emasculation pollination was carried out on the bell stage flowers. Cover sulfuric acid paper bag immediately after pollination and change mesh bag after 7 days.

[0034] B. Fruit picking and low temperature layering treatment: such as figure 1 As shown in C, young hybrid fruits were harvested at the best stage for hybrid embryo rescue (PF >0.5 at the hard nucleus stage)....

Embodiment 2

[0045] The hybrid embryo rescue technology between sweet cherry and Chinese cherry in the southern region comprises the following steps:

[0046] A. Collect pollen and pollinate: Collect the natural flowers of Chinese cherry 'Gejiawu' bell flowering period. The anthers were peeled off indoors and dried in the shade at 25°C. After the anthers cracked and loosened the powder, they were collected in small glass bottles, sealed and stored in a refrigerator at 4°C for future use.

[0047] Using the sweet cherry 'Chaoyang No.1' as the female parent, artificial non-emasculation pollination was carried out on the bell stage flowers. Cover sulfuric acid paper bag immediately after pollination and change mesh bag after 7 days.

[0048] B. Fruit picking and low-temperature stratification treatment: The best time to collect 'Chaoyang No. 1'×'Gejiawu' embryos is 37 days after pollination. Put the fresh fruits in a transparent plastic fresh-keeping box and store them in a 4 ℃ low-temperat...

Embodiment 3

[0059] The hybrid embryo rescue technology between sweet cherry and Chinese cherry in the southern region comprises the following steps:

[0060] A. Collect pollen and pollinate: Collect the natural flowers of Chinese cherry 'Black Pearl' bell flowering period. The anthers were peeled off indoors and dried in the shade at 25°C. After the anthers cracked and loosened the powder, they were collected in small glass bottles, sealed and stored in a refrigerator at 4°C for future use.

[0061] With the sweet cherry 'Chaoyang No. 1' as the female parent, the flowers at the bell stage were artificially pollinated without emasculation. Immediately after pollination, cover the sulfuric acid paper bag and change the mesh bag after 7 days.

[0062] B. Fruit harvesting and low-temperature stratification treatment: The best time to save 'Chaoyang No. 1'×'Black Pearl' embryos is 37 days after pollination. After harvesting, fresh fruits should be placed in transparent plastic fresh-keeping ...

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Abstract

The invention relates to the field of cross breeding of plants and particularly relates to an interspecific hybrid embryo rescuing and seedling forming method of sweet cherries in southern China and Chinese cherries. The method combines advantages of European sweet cherries and the Chinese cherries, and takes the 'European sweet cherries' as a female parent and the 'Chinese cherries' as a male parent to carry out interspecific distant hybridization. Aiming at the abortion problem of a distant hybrid embryo, the hybrid embryo is subjected to embryo rescuing. A proper sampling period of the embryo rescuing of each hybrid combination, concentrations and ratios of respective exogenous growth regulators for hybrid young embryo germination, subculture propagation and rooting culture, and a proper acclimatization and transplanting method are determined. By applying an interspecific hybrid embryo rescuing system of the sweet cherries in southern China and the Chinese cherries, which is established by the technology, the embryo germination rate reaches 80.00 percent, the multiplication coefficient of subculture hybridized new shoots reaches 5.0, the rooting rate of rooting culture is 90.91percent, the growth vigor of embryo culture seedlings is good and the seedlings grow robustly and have green leaves, and the acclimatization and transplanting survival rate reaches 77.27 percent.

Description

technical field [0001] The invention relates to the field of plant hybrid breeding, in particular to a method for rescuing seedlings from interspecific hybrid embryos of sweet cherries and Chinese cherries suitable for planting in southern regions. Background technique [0002] Cherry is a member of the genus Prunus (Rosaceae) in the Rosaceae (Rosaceae) Prunus) Cherry subgenus ( Cerara s) Arbor, the fruit tastes sweet and has high nutritional value, among which the iron element content ranks first in the fruit, known as "the first branch of all fruits". Restricted by geographical and climatic conditions, the main production areas of sweet cherries in my country have been concentrated in northern regions such as Dalian and Yantai for a long time. Due to factors such as high temperature and high humidity in summer and insufficient cooling capacity in winter, sweet cherries are only flowers but no fruit and no economy. Yield. In recent years, the planting of sweet cherries i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/02A01H4/00A01G17/00
CPCA01G17/005A01H1/02A01H4/001A01H4/008A01H1/027
Inventor 吴延军宋全清戚行江袁玥周慧芬武凯翔丁晓瑜
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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