The invention discloses a method for culturing pleurotus eryngii. The method comprises the following steps of: selecting saw powder of alfalfa growing in organic soil, wild nuts in mountain and wild trees in mountain, mixing, fermenting, sterilizing, and inoculating the sterilized fermentation product to pleurotus eryngii hypha to culture to obtain mature pleurotus eryngii. By adopting the alfalfa growing in the organic soil, the problem that the alfalfa growing in common soil contains chemical fertilizer, pesticide residue and the like is solved, and harm of chemicals to a human body is solved; meanwhile, because the alfalfa is selected as one of the culture medium materials for culturing the pleurotus eryngii, the germination rate of the pleurotus eryngii can be increased by using the alfalfa to culture the pleurotus eryngii; and because the alfalfa is used as one of the culture medium materials, the growth speed of hypha can be increased, so that the production efficiency of the pleurotus eryngii is increased, and the economic benefit of the pleurotus eryngii is increased. The method has the advantages of achieving the best sterilization effect, saving the sterilization time, being simple and easy in material selection, saving a large amount of labor, reducing the production cost and increasing the economic benefit.