Efficient fermentation production process of sodium gluconate
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A technology of sodium gluconate and production process is applied in the field of high-efficiency fermentation production process of sodium gluconate to achieve the effects of saving bacterial culture and improving production efficiency
Inactive Publication Date: 2014-07-02
山东福洋生物科技股份有限公司
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[0005] In summary, at present this area lacks sodium gluconate continuous fermentation method, therefore urgently needs to develop a kind of continuous, stable, high yield, low energy consumption, The method for the sodium gluconate continuous fermentation of on-line automatic control
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Embodiment 1
[0023] The first step, seed cultivation: Add 2.5kg of glucose, 60g of corn steep liquor, 5.2g of potassium dihydrogen phosphate, 2g of urea, and 2ml of antifoaming agent into a 15L cultivation tank. To 38°C, add 250ml eggplant bottle bacterial suspension, control dissolved oxygen (DO) ≥ 30, cultivate for 15h to obtain seed liquid.
[0024] The second step, fermentation: add 4.0kg of glucose, 2.8g of magnesium sulfate, 1.4g of urea, and 2ml of defoamer to a 50L fermenter, set the volume to 13L, sterilize at 115°C for 15 minutes, then lower it to 38°C, and culture the well The seeds are added to the fermenter in a ratio of 20%, and the pH is adjusted and controlled to 5.4 with 350g / L NaOH to start fermentation.
[0025] The third step, continuous fermentation:
[0026] (1) Ingredients: 14kg of 500g / L glucose, 50g of corn steep liquor, 12.5g of magnesium sulfate, 6.8g of urea, and 1ml of defoamer were added to a 30L feeding tank. To 38 ℃, aseptic transfer to serum bottles for l...
Embodiment 2
[0037] The first step, seed cultivation: Add 2.5kg of glucose, 60g of corn steep liquor, 5.2g of potassium dihydrogen phosphate, 2g of urea, and 2ml of antifoaming agent into a 15L cultivation tank. To 38°C, add 250ml eggplant bottle bacterial suspension, control dissolved oxygen (DO) ≥ 30, cultivate for 15h to obtain seed liquid.
[0038] The second step, fermentation: add 4.0kg of glucose, 2.8g of magnesium sulfate, 1.4g of urea, and 2ml of defoamer to a 50L fermenter, set the volume to 13L, sterilize at 115°C for 15 minutes, then lower it to 38°C, and culture the well The seeds are added to the fermenter in a ratio of 20%, and the pH is adjusted and controlled to 5.4 with 350g / L NaOH to start fermentation.
[0039] The third step, continuous fermentation:
[0040] (1) Ingredients: Add 14kg of 500g / L glucose, 100g of corn steep liquor, 25g of magnesium sulfate, 13.6g of urea, and 2ml of defoamer to a 30L feeder tank, set the volume to 20L, sterilize at 115°C for 30 minutes,...
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Abstract
The invention relates to an efficient fermentation production process of a sodium gluconate. The efficient fermentation production process comprises the first step of seed culturing, the second step of inoculated fermentation, the third step of continuous fermentation, the fourth step of filtering, the fifth step of decoloring, the sixth step of crystallization, the seventh of separation and drying and the eighth step of packaging. In the third step, namely, the continuous fermentation step, when the online measured concentration of a sodium gluconate reducing sugar is less than or equal to 50g / L according to the terminal stage of fermentation of the second step, a glucose liquid and a nutrient are automatically replenished by 500g / L, and simultaneously, a fermentation liquor is discharged to a buffer tank from the bottom of a fermentation tank, and meanwhile, NaOH (in a content of 350g / L) is also replenished. The continuous fermentation step of the efficient fermentation production process has the advantages that the glucose liquid and the nutrient are replenished under online automatic control, long-term continuous fermentation of the glucose is realized, the time of seed culture and fermentation liquor sterilization is saved, and the production efficiency is improved.
Description
technical field [0001] The invention relates to the field of bioengineering, in particular to a high-efficiency fermentation production process of sodium gluconate. Background technique [0002] Gluconic acid is widely used in food, medicine, chemical industry, water treatment, construction and other industries as a bulking agent, coagulant, chelating agent, and sour agent; gluconate (such as with sodium, calcium, zinc, ferrous and other metal oxides) Synthetic gluconate) can be used as food additives and nutritional supplements. [0003] The production of gluconic acid mainly includes microbial fermentation, electrolysis and catalytic oxidation. Due to the mild conditions and obvious energy saving of microbial method, it is widely used. Among them, microbial fermentation has fungal fermentation and bacterial fermentation process. Since Boutroux first used the oxidation of microorganisms to oxidize glucose into gluconic acid, the fermentation process of gluconic acid and ...
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