Culturing medium and fermentation method for producing erythrocin through fermentation of streptomyces erythreus

Abstract

The invention relates to a culturing medium and fermentation method for producing erythrocin through fermentation of streptomyces erythreus. A primary seed medium, a secondary seed medium and a fermentation medium for producing erythrocin through fermentation of streptomyces erythreus all contains sucrose and a corn steep liquor. According to the invention, the corn steep liquor, urea, sucrose, and other intermediate-acting carbon sources and nitrogen sources are adopted to substitute original slow-acting carbon sources and nitrogen sources; moreover, the corn steep liquor, urea, and sucrose are soluble in water, so that the viscosity of the fermentation liquor is lowered, the dissolved oxygen is increased, the utilization speed is increased, especially during the later period of fermentation, the solid residue at the end of the fermentation is reduced, the bacteria dregs and the BOD content and the COD contend in the waste water are all greatly reduced, energy-reserving and consumption-lowering are both realized, and the good effects of treatment from the source and clean production are achieved.

Description

technical field

[0001] The invention belongs to the technical field of biological fermentation, and in particular relates to a culture medium and a fermentation method for producing erythromycin by fermentation of Streptomyces erythromycin. Background technique

[0002] Erythromycin (Er) is a class of macrolide antibiotics, mainly including erythromycin A (ErA), erythromycin B (ErB) and erythromycin C (ErC). The antibacterial activity of B and C is the strongest, and the antibacterial activity of B and C is lower. In the prior art, in the process of fermenting and producing erythromycin with Streptomyces erythromycin as the fermentation strain, a large amount of late-acting soybean cake powder, starch, etc. are used in its seed medium, fermentation medium, and feeding medium. Carbon and nitrogen sources make the solid content of the culture medium greater than 6%, the solid content of insoluble carbon sources is greater than 3%, and the total solid content is greater than 9...

Examples

Embodiment 1

[0027] 1 Primary seed culture: Inoculate the slant spores in the sterilized primary seed medium and cultivate them at a temperature of 33-35°C, with a ventilation rate of 1: 0.4-0.6v / v / min, stirring, and culturing for 45-60 hours to obtain First-class seed liquid.

[0028] The composition of primary seed medium: 2% sucrose, 1% corn steep liquor, 0.15% ammonium sulfate, 0.5% calcium carbonate; at 118±2°C, keep the pressure for 20 minutes.

[0029] 2. Secondary seed cultivation: Inoculate the primary seed solution with 15% of the inoculum amount into the sterilized secondary seed medium for cultivation. The cultivation temperature is 33-35°C, and the ventilation rate is 1:1.0-1.2v / v / min. Stir and cultivate for 24-26 hours to obtain the secondary seed solution.

[0030] Secondary seed medium composition: 1.4% sucrose, 1.5% starch, 2.0% urea, 0.8% corn steep liquor, 0.4% ammonium sulfate, 0.8% calcium carbonate, 0.5% vegetable oil; pressurize for 20 minutes at 118±2°C.

[0031] ...

Embodiment 2

[0038] 1 Primary seed culture: inoculate the slant spores in the sterilized primary seed medium and cultivate them at a temperature of 34-36°C, with a ventilation rate of 1: 0.7-0.8v / v / min, stirring, and culturing for 45-60 hours to obtain First-class seed liquid.

[0039] The composition of primary seed medium: 5.0% sucrose, 3.0% corn steep liquor, 0.5% ammonium sulfate, 0.7% light calcium carbonate; pressurize for 20 minutes at 118±2°C.

[0040] 2. Secondary seed cultivation: Inoculate the primary seed solution with 10% of the inoculum amount into the sterilized secondary seed medium for cultivation. The cultivation temperature is 32-34°C, and the ventilation rate is 1: 0.8-1.0v / v / min. Stir and cultivate for 20-24 hours to obtain the secondary seed solution.

[0041] The composition of the secondary seed medium: 0.8% sucrose, 0.8% starch, 1.0% urea, 0.4% corn steep liquor, 0.1% ammonium sulfate, 0.5% calcium carbonate, and 0.2% vegetable oil; at 118±2°C, keep the pressure f...

Embodiment 3

[0049] 1 Primary seed culture: inoculate the slant spores in the sterilized primary seed medium and cultivate them at a temperature of 32-34°C, with a ventilation rate of 1: 0.6-0.7v / v / min, stirring, and culturing for 45-60 hours to obtain First-class seed liquid.

[0050] The composition of primary seed medium: 3.3% sucrose, 2% corn steep liquor, 0.1% ammonium sulfate, 0.9% light calcium carbonate; pressurize for 20 minutes at 118±2°C.

[0051] 2. Secondary seed cultivation: Inoculate the primary seed solution with 5% of the inoculum amount into the sterilized secondary seed medium for cultivation. The cultivation temperature is 34-36°C, and the ventilation rate is 1:1.0-1.1v / v / min. Stir and cultivate for 26-30 hours to obtain the secondary seed solution.

[0052] Secondary seed medium composition: 1.2% sucrose, 1% starch, 2.5% urea, 0.5% corn, 0.25% ammonium sulfate, 0.9% calcium carbonate, 0.3% vegetable oil; hold at 118±2°C for 20 minutes.

[0053] 3. Fermentation cultur...

technical field

[0001] The invention belongs to the technical field of biological fermentation, and in particular relates to a culture medium and a fermentation method for producing erythromycin by fermentation of Streptomyces erythromycin. Background technique

[0002] Erythromycin (Er) is a class of macrolide antibiotics, mainly including erythromycin A (ErA), erythromycin B (ErB) and erythromycin C (ErC). The antibacterial activity of B and C is the strongest, and the antibacterial activity of B and C is lower. In the prior art, in the process of fermenting and producing erythromycin with Streptomyces erythromycin as the fermentation strain, a large amount of late-acting soybean cake powder, starch, etc. are used in its seed medium, fermentation medium, and feeding medium. Carbon and nitrogen sources make the solid content of the culture medium greater than 6%, the solid content of insoluble carbon sources is greater than 3%, and the total solid content is greater than 9...

Examples

Embodiment 1

[0027] 1 Primary seed culture: Inoculate the slant spores in the sterilized primary seed medium and cultivate them at a temperature of 33-35°C, with a ventilation rate of 1: 0.4-0.6v / v / min, stirring, and culturing for 45-60 hours to obtain First-class seed liquid.

[0028] The composition of primary seed medium: 2% sucrose, 1% corn steep liquor, 0.15% ammonium sulfate, 0.5% calcium carbonate; at 118±2°C, keep the pressure for 20 minutes.

[0029] 2. Secondary seed cultivation: Inoculate the primary seed solution with 15% of the inoculum amount into the sterilized secondary seed medium for cultivation. The cultivation temperature is 33-35°C, and the ventilation rate is 1:1.0-1.2v / v / min. Stir and cultivate for 24-26 hours to obtain the secondary seed solution.

[0030] Secondary seed medium composition: 1.4% sucrose, 1.5% starch, 2.0% urea, 0.8% corn steep liquor, 0.4% ammonium sulfate, 0.8% calcium carbonate, 0.5% vegetable oil; pressurize for 20 minutes at 118±2°C.

[0031] ...

Embodiment 2

[0038] 1 Primary seed culture: inoculate the slant spores in the sterilized primary seed medium and cultivate them at a temperature of 34-36°C, with a ventilation rate of 1: 0.7-0.8v / v / min, stirring, and culturing for 45-60 hours to obtain First-class seed liquid.

[0039] The composition of primary seed medium: 5.0% sucrose, 3.0% corn steep liquor, 0.5% ammonium sulfate, 0.7% light calcium carbonate; pressurize for 20 minutes at 118±2°C.

[0040] 2. Secondary seed cultivation: Inoculate the primary seed solution with 10% of the inoculum amount into the sterilized secondary seed medium for cultivation. The cultivation temperature is 32-34°C, and the ventilation rate is 1: 0.8-1.0v / v / min. Stir and cultivate for 20-24 hours to obtain the secondary seed solution.

[0041] The composition of the secondary seed medium: 0.8% sucrose, 0.8% starch, 1.0% urea, 0.4% corn steep liquor, 0.1% ammonium sulfate, 0.5% calcium carbonate, and 0.2% vegetable oil; at 118±2°C, keep the pressure f...

Embodiment 3

[0049] 1 Primary seed culture: inoculate the slant spores in the sterilized primary seed medium and cultivate them at a temperature of 32-34°C, with a ventilation rate of 1: 0.6-0.7v / v / min, stirring, and culturing for 45-60 hours to obtain First-class seed liquid.

[0050] The composition of primary seed medium: 3.3% sucrose, 2% corn steep liquor, 0.1% ammonium sulfate, 0.9% light calcium carbonate; pressurize for 20 minutes at 118±2°C.

[0051] 2. Secondary seed cultivation: Inoculate the primary seed solution with 5% of the inoculum amount into the sterilized secondary seed medium for cultivation. The cultivation temperature is 34-36°C, and the ventilation rate is 1:1.0-1.1v / v / min. Stir and cultivate for 26-30 hours to obtain the secondary seed solution.

[0052] Secondary seed medium composition: 1.2% sucrose, 1% starch, 2.5% urea, 0.5% corn, 0.25% ammonium sulfate, 0.9% calcium carbonate, 0.3% vegetable oil; hold at 118±2°C for 20 minutes.

[0053] 3. Fermentation cultur...