Culturing medium and fermentation method for producing erythrocin through fermentation of streptomyces erythreus

Abstract

The invention relates to a culturing medium and fermentation method for producing erythrocin through fermentation of streptomyces erythreus. A primary seed medium, a secondary seed medium and a fermentation medium for producing erythrocin through fermentation of streptomyces erythreus all contains sucrose and a corn steep liquor. According to the invention, the corn steep liquor, urea, sucrose, and other intermediate-acting carbon sources and nitrogen sources are adopted to substitute original slow-acting carbon sources and nitrogen sources; moreover, the corn steep liquor, urea, and sucrose are soluble in water, so that the viscosity of the fermentation liquor is lowered, the dissolved oxygen is increased, the utilization speed is increased, especially during the later period of fermentation, the solid residue at the end of the fermentation is reduced, the bacteria dregs and the BOD content and the COD contend in the waste water are all greatly reduced, energy-reserving and consumption-lowering are both realized, and the good effects of treatment from the source and clean production are achieved.

Description

technical field [0001] The invention belongs to the technical field of biological fermentation, and in particular relates to a culture medium and a fermentation method for producing erythromycin by fermentation of Streptomyces erythromycin. Background technique [0002] Erythromycin (Er) is a class of macrolide antibiotics, mainly including erythromycin A (ErA), erythromycin B (ErB) and erythromycin C (ErC). The antibacterial activity of B and C is the strongest, and the antibacterial activity of B and C is lower. In the prior art, in the process of fermenting and producing erythromycin with Streptomyces erythromycin as the fermentation strain, a large amount of late-acting soybean cake powder, starch, etc. are used in its seed medium, fermentation medium, and feeding medium. Carbon and nitrogen sources make the solid content of the culture medium greater than 6%, the solid content of insoluble carbon sources is greater than 3%, and the total solid content is greater than 9...

AI Technical Summary

Problems solved by technology

In the prior art, in the process of fermenting and producing erythromycin with Streptomyces erythromycin as the fermentation strain, a large amount of late-acting soybean cake powder, starch, etc. are used in its seed medium, fermentation medium, and feeding medium. Carbon and nitrogen sources make the solid content of the culture medium greater than 6%, the solid content of insoluble carbon sources is greater than 3%, and the total solid content is greater than 9%. The existence of these solid materials increases the difficulty of sterilization and the cost of raw materials, and increases the fermentation process. The viscosity of the liquid limits the oxygen supply during the fermentation process, and it cannot be fully utilized by the bacteria during the fermentation process, resulting in a solid content of more than 12% in the fermentation liquid at the end of the fermentation, which increases the consumption of raw materials, auxiliary facilities, and materials, and increases the fermentation rate. Difficulty and cost of filtration, purification and wastewater treatment in downstream liquid treatment