A non-thermal processing method of instant bird's nest comprises the steps of soaking, to be specific, soaking bird's nest in a vessel filled with purified water for 2-8 hours; separating, to be specific, separating the soaked bird's nest from the purified water; picking out feather, to be specific, picking feather out of the soaked bird's nest to obtain clean bird's nest; filling, to be specific,weighing a certain amount of the clean bird's nest, and loading into an EVOH (ethylene vinyl alcohol) bottle; adding auxiliary materials, to be specific, adding auxiliary materials into the EVOH bottle; sealing, to be specific, allowing a carbon dioxide generator to remove oxygen at an opening of the EVOH bottle, and rapidly sealing the opening to obtain semi-finished bird's nest; sterilizing, tobe specific, raising the temperature of a sterilization pot to 70-80 DEG C, and boiling the semifinished bird's nest in the sterilization pot for 15-45 min; allowing reaction to occur, to be specific, soaking the boiled semifinished bird's nest in a pressure transmission medium in a reactor of a high static pressure device, raising the pressure to 300-600 MP, and holding the pressure for 3-30 minto obtain instant bird's nest. Absorption by the human body is facilitated, and the processing is convenient.