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Fermentation method for erythromycin

A fermentation method and tetracycline technology, applied in the directions of fermentation, microorganism-based methods, biochemical equipment and methods, etc., can solve the problems of increasing the difficulty of fermentation process operation, incomplete utilization of delayed carbon sources, waste of raw materials, etc. The effect of shortening the bacteria time, reducing the amount of starch, and reducing the cost of raw materials

Inactive Publication Date: 2015-11-04
NINGXIA QIYUAN PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the biological fermentation process, a large amount of starch is used as the base material, and a large amount of starch sugar is added during the fermentation process to maintain the growth of the bacteria and the synthesis of products. In this way, there is an incomplete utilization of late-acting carbon sources in the late stage of fermentation. This phenomenon not only causes waste of raw materials, but also causes difficulties in subsequent extraction due to a large amount of residual carbon sources when the fermentation broth is put into the tank. At the same time, it needs to be pretreated before the addition of starch sugar to speed up its decomposition and utilization, which further increases the Difficulty in operation of fermentation process

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] 1.1 Seed culture: Inoculate the slant spores prepared by conventional methods into the sterilized seed medium for cultivation.

[0026] The composition of seed medium: starch 2.5%, soybean powder 2.5%, yeast powder 0.5%, corn steep liquor 0.5%, ammonium sulfate 0.3%, light calcium carbonate 0.3%, sodium bromide 0.2%, and the rest is water. Prepare and sterilize according to the ratio of weight to volume.

[0027] Culture conditions: culture temperature 32°C, ventilation rate 1: 0.4 v / v / min with stirring, culture for 22 hours to obtain seed liquid.

[0028] 1.2 Fermentation culture: Inoculate the seed liquid into the sterilized fermentation medium with 18% inoculum amount for cultivation.

[0029] Composition of fermentation medium: starch 3.0%, dextrin 1.5%, glucose 1.5%, soybean powder 2.0%, corn steep liquor 2.0%, ammonium sulfate 0.5%, calcium carbonate 0.5%, sodium bromide 0.2%, M-accelerator 0.002% , and the rest is water. Prepare and sterilize according to the ...

Embodiment 2

[0038] 2.1 Seed culture: Inoculate the slant spores prepared by conventional methods into the sterilized seed medium for seed culture.

[0039] The composition of seed medium: starch 4.0%, soybean powder 3.5%, yeast powder 1.5%, corn steep liquor 1.0%, ammonium sulfate 0.7%, light calcium carbonate 0.5%, sodium bromide 0.35%, and the rest is water. Prepare and sterilize according to the ratio of weight to volume.

[0040] Culture conditions: culture temperature 35°C, ventilation rate 1: 0.8v / v / min with stirring, cultivate for 27.8hr to obtain seed liquid.

[0041] 2.2 Fermentation culture: Inoculate the seed liquid into the sterilized fermentation medium according to 24% inoculation amount to carry out fermentation sheet culture.

[0042] Composition of fermentation medium: starch 4.5%, dextrin 2.5%, glucose 2.5%, soybean powder 3.0%, corn steep liquor 3.0%, ammonium sulfate 0.8%, calcium carbonate 0.7%, sodium bromide 0.35%, M-accelerator 0.003% , and the rest is water. Pr...

Embodiment 3

[0051]3.1 Seed culture: Inoculate the slant spores prepared by conventional methods into the sterilized seed medium for seed culture.

[0052] The composition of seed medium: starch 3.5%, soybean powder 3.0%, yeast powder 1.0%, corn steep liquor 0.8%, ammonium sulfate 0.5%, light calcium carbonate 0.4%, sodium bromide 0.3%, and the rest is water. Prepare and sterilize according to the ratio of weight to volume.

[0053] Culture conditions: culture temperature 33°C, ventilation rate 1: 0.8v / v / min with stirring, cultivate for 26hr to obtain seed liquid.

[0054] 3.2 Fermentation culture: Inoculate the seed liquid into the sterilized fermentation medium according to 20% inoculum amount for fermentation culture.

[0055] Composition of fermentation medium: starch 4.0%, dextrin 2.0%, glucose 2.0%, soybean powder 2.5%, corn steep liquor 2.5%, ammonium sulfate 0.7%, calcium carbonate 0.6%, sodium bromide 0.3%, M-accelerator 0.0015% , and the rest is water. Prepare and sterilize ac...

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Abstract

The invention relates to a fermentation method for erythromycin. According to the method, a mixture containing glucose is used as a feed supplement carbon source, and adjustment of fermentation yeast basis and supplement technological adjustment of the carbon source are further matched. According to the novel erythromycin fermentation technology, the erythromycin fermentation yeast basis formula used in the glucose supplement technology and the control condition of the carbon source are given in the fermentation process. The fermentation metabolism process is changed by adjusting the fermentation yeast basis formula and the carbon source supplement type, and therefore the purposes of saving raw material cost of the carbon source, improving the fermentation level, weakening extraction difficulty, saving energy and reducing consumption are achieved.

Description

technical field [0001] The invention relates to the technical field of biological fermentation, in particular to the field of antibiotic production by actinomycetes, in particular to the technical field of tetracycline fermentation. Background technique [0002] Tetracycline (erythromycin, Er) is a class of tetracyclic antibiotics, which is often produced by biological fermentation using Streptomyces aureus as the fermentation strain. In the biological fermentation process, a large amount of starch is used as the base material, and a large amount of starch sugar is added during the fermentation process to maintain the growth of the bacteria and the synthesis of products. In this way, there is an incomplete utilization of late-acting carbon sources in the late stage of fermentation. This phenomenon not only causes waste of raw materials, but also causes difficulties in subsequent extraction due to a large amount of residual carbon sources when the fermentation broth is put in...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P29/00C12R1/49
Inventor 郭海燕孙瑞君崔莉张志
Owner NINGXIA QIYUAN PHARMA
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