2results about How to "Simple requirements" patented technology

The application discloses a kind of high-strength plastic β titanium alloy strip and whole-process preparation method, the β titanium alloy strip is composed of the following mass percentage components: Cr 7.7%~8.3%, Mo 4.9%~5.5%, V 4.9%~5.5%, Al 2.7%~3.3%, Fe≤0.20%, C≤0.03%, N≤0.02%, H≤0.010%, O≤0.10%, and the rest is Ti;The preparation method comprises: one, forging;Two, hot rolling;Three, cold rolling;Four, heat treatment.The β titanium alloy strip and whole-process preparation method of the application are overall planning from component design to final heat treatment whole-process procedure, improve production efficiency, shorten preparation time, and the β titanium alloy strip solid solution state microstructure is equiaxed β fine grain structure, strength-plasticity matching is high, horizontal longitudinal organization performance is uniform, with excellent comprehensive mechanical properties, applicable to metal processing field.

The application provides a content extraction method based on cell membrane capacitance change, and belongs to the field of cell-level micromanipulation technology, and comprises the following steps: S1, a change relationship between cell membrane capacitance and cell membrane surface area is established; S2, a change relationship between cell membrane surface area and volume is approximately established; S3, high-resistance sealing and membrane rupture of cells are completed, constant voltage cell content extraction operation is performed, and cell membrane capacitance values in the extraction process are obtained in real time through capacitance fitting; S4, content extraction amount is estimated; S5, actual cytoplasm extraction amount is calculated based on a rotary L-shaped electrode, the relationship model established in S2 is corrected, and finally, quantitative extraction of cell contents is realized. The application utilizes an electrical model of cell membrane capacitance, realizes real-time feedback of cell content extraction amount, realizes quantitative control of content extraction, can realize a cytoplasm extraction success rate of 62.5% in the case of a whole cell patch clamp structure, and the average cytoplasm extraction amount control error is less than 6% of the total cell volume.