The invention discloses a creation method of switchgrass mutants. The creation method comprises the following steps: after a lots of switchgrass multiple shoots are cultured in a tissue culture manner, stripping out individual multiple shoots, cutting off the upper sheaths after acclimatization under natural conditions, performing soaking treatment on the parts 1.5-2cm above the left base parts by use of a solution of ethylmethane sulfonate (EMS) for 3 hours, and after the completion of the soaking treatment, transplanting the multiple shoots of which the sheaths are cut off into a culture medium for culture, and after complete rooting, transplanting to a greenhouse or land, and screening out the mutants according to the phenotypes of the switchgrass. The method is capable of realizing large-scale processing of the mutational original acceptor material and thus increasing the basic number of the variants, and meanwhile, a near-isogenic line material only different from the original acceptor material in target traits can be obtained; and in addition, the mutagenesis process is simple and feasible, the processing cost is relatively low, and the breeding period can be effectively shortened; in short, the method is suitable for creating new materials for the modern biotechnology research of the switchgrass.