Method for increasing seedless grape crossed embryo rescue seedling rate

Abstract

The invention belongs to the technical field of plant breeding, and discloses a method for increasing the seedless grape crossed embryo rescue seedling rate. According to the method, before abortion of seedless grape crossed embryos, physical measures of pinching and girdling are taken to promote in-vivo development of ovules and embryos, the appropriate time for embryo abortion rescue sampling of two Southern seedless grape hybridized combinations is confirmed, and content such as culture medium additives for culturing ovules and germinating embryos, test tube seedling dual-bud stem inoculation subculturing and subculturing culture medium additives are confirmed. Due to the adoption of the method, the seedless grape crossed embryo developing rate is high, the embryo rescue seedlings are tidy and strong, the embryo developing rate is up to 41.7%, the seedling rate is up to 18.3%, the subculturing culture seedling rate is up to 92%, the rate of strong and healthy seedlings is 81.8%, the test tube seedling transplanting survival rate is up to 97%, and the cardinal number of the crossed filial generation embryo rescue seedlings is greatly increased, so that a relatively great number of seedless crossed generation plant groups can be obtained, the seedless grape crossed embryo seedling rate in southern areas can be increased, and the method is high in operability and easy to master.

Description

technical field [0001] The invention relates to the technical field of plant breeding, in particular to a method for improving seedless grape hybrid embryo rescue into seedlings. Background technique [0002] Seedless grapes are popular among consumers due to their convenience, and occupy an important position in the production of table grapes in the world. More than 80% of the table grapes in the United States are seedless varieties. However, in terms of grape breeding, since seedless grapes have no seeds, it is impossible to obtain hybrid offspring plants as female parents. Very low, and the breeding cycle is long. In the 1990s, the development of embryo rescue technology brought a new approach to seedless grape breeding, making it possible for seedless grapes to be used as female parents for hybrid breeding, which not only greatly increased the probability of seedless offspring, but also greatly shortened the breeding time. Cycle, to a certain extent, solves the difficu...

AI Technical Summary

Problems solved by technology

However, in the process of embryo rescue, the genotype of the variety, the time of sampling and inoculation, the type and phase of the medium, plant growth regulators, carbon sources, culture environment, transplanting methods, etc. will all have an impact on the germination rate and seedling rate of embryo rescue. , and the interaction of various factors, and the differences between the north and the south make the southern grape mature early and the best period of embryo abortion is different, which increases the difficulty of constructing the seedless grape embryo rescue technology system, resulting in the seedless grape being the female parent. The embryo development rate in the fruit after hybridization is low, and the lack of embryo rescue has become a bottleneck restricting the breeding efficiency of seedless grapes in the south. How to increase the seedling rate of seedless grape embryo rescue breeding and expand the population of embryo rescue plants has become a key issue for grape breeders. Striving Direction