Method for improving cattle cloning efficiency by micro ribonucleic acid

A technology of micro ribonucleic acid and high efficiency, which is applied in the direction of botany equipment and methods, biochemical equipment and methods, applications, etc., can solve problems such as bovine cloning that has not been seen, and achieve the purpose of improving cloning efficiency, improving development rate and quality, and avoiding The effect of secondary damage

Active Publication Date: 2016-11-09
NORTHWEST A & F UNIV
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Problems solved by technology

However, so far, there has been no relevant report on improving the efficiency of bovine cloning by reducing the level of histone methylation in cloned embryos

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  • Method for improving cattle cloning efficiency by micro ribonucleic acid
  • Method for improving cattle cloning efficiency by micro ribonucleic acid
  • Method for improving cattle cloning efficiency by micro ribonucleic acid

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Embodiment Construction

[0025] The present invention will be described in detail below in conjunction with the accompanying drawings and embodiments, which are explanations rather than limitations of the present invention.

[0026] miR-125b is a microRNA enriched in early embryos, which can inhibit the histone methyltransferase Suv39h1, thereby effectively removing the 3-methylation (H3K9me3) of histone H3 lysine K9 (H3K9me3) epigenetic modification. Transfection of miR-125b can effectively reduce the level of histone H3K9me3 epigenetic modification in cloned embryos during the zygotic activation period, so as to promote the normal development of somatic cell cloned embryos. Accordingly, the present invention adds bta-miR-125b mimic to the electrofusion solution of bovine somatic cell nuclear transfer, and can effectively introduce bta-miR-125b mimic into bovine somatic cell cloned embryos, and realize the transfection of miR- 125b. Thereby reducing the level of histone H3K9me3 epigenetic modificati...

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Abstract

The invention discloses a method for improving cattle cloning efficiency by micro ribonucleic acid. bta-miR-125b mimic is added into electrical fusion solution of somatic cell nucleus transfer and can be effectively led into an embryo, the histone H3K9me3 epigenetic modification level of a somatic cell cloning embryo in a zygotic activation period can be effectively weakened while secondary damage of transfection is avoided, and the developmental rate and the quality of a cattle somatic cell cloning blastaea can be obviously improved.

Description

technical field [0001] The invention belongs to the technical field of animal somatic cell cloning, and relates to a method for processing bovine somatic cell cloned embryos constructed based on somatic cell nuclear transfer, in particular to a method for improving bovine cloning by using microRNA to weaken the histone methylation level in cloned embryos. efficient method. Background technique [0002] Somatic cell cloning is a technology with great research and commercial value, which can be applied to therapeutic cloning, disease models, human organ transplantation, animal transgenic research, protection of endangered animal breeds, and expansion of superior livestock breeds. But so far the efficiency of somatic cell cloning is still not high, which significantly restricts the wide application of this technology. [0003] At present, it is believed that the main reason for the low cloning efficiency is that the nucleus of the donor somatic cell is not completely reprogram...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/877C12N5/073
CPCC12N5/0604C12N15/8771
Inventor 张景程张涌屈鹏翔王勇胜
Owner NORTHWEST A & F UNIV
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