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Method for improving efficiency of raisin grape breeding

A seedless grape, efficient technology, applied in horticultural methods, botanical equipment and methods, chemicals for biological control, etc., which can solve the problem of limited number of hybrid progeny, and very different ratios

Inactive Publication Date: 2008-08-13
NORTHWEST A & F UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the number ratio of hybrid progeny obtained by seedless grape seeding technology is still very limited, which is still far from the practice of hybrid breeding

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] The preparation of embodiment 1 chlormequat solution

[0040] Chlormequat was first dissolved to make 1000mg.L -1 Mother liquor: Weigh 1g of chlormequat (purchased from Shanghai Jiafeng Horticultural Supplies Co., Ltd., purity 98%), dissolve it with about 100ml of distilled water, and dilute to 1000ml with distilled water, which is 1000mg.L -1 The mother liquor, stored at room temperature, will be used in the near future. When preparing 50mg.L -1 For chlormequat solution, use a measuring cylinder to measure 50ml of the mother solution, set the volume to 1000ml, then add 1ml of Tween-20, which is 0.1% concentration, and mix well, which is 50mg.L -1 chlormequat solution.

[0041] Prepare 500mg.L -1 For chlormequat solution, use a measuring cylinder to measure 500ml of mother liquor, set the volume to 1000ml, then add 1ml of Tween-20, which is 0.1% concentration, and mix well, which is 500mg.L -1 Chlormequat solution; to prepare 50 and 500mg.L -1 Concentrations betwe...

Embodiment 2

[0042] The preparation of embodiment 2 6-BA solution

[0043] 6-BA is also first formulated to 1000mg.L -1 Mother liquor: Weigh 1g 6-BA (produced by Shanghai Chemical Reagent Company, purity 99.6%), dissolve with 50-100ml of dilute hydrochloric acid, gradually add distilled water, stir while adding to prevent precipitation, and distill the volume to 1000ml with distilled water, And use NaOH to adjust the pH value to about 7.0, which is 1000mg.L -1 The mother liquor, stored at room temperature, will be used in the near future. Prepare 10mg.L -1 For the 6-BA solution, measure 10ml of the mother liquor with a graduated cylinder, set the volume to 1000ml, then add 1ml of Tween-20, which is 0.1% concentration, and mix well, which is 10mg.L -1 6-BA solution.

[0044] Prepare 100mg.L -1 For the 6-BA solution, measure 100ml of mother liquor with a graduated cylinder, set the volume to 1000ml, then add 1ml of Tween-20, which is 0.1% concentration, and mix well, which is 100mg.L -...

Embodiment 3

[0046]10-20 days before the flowering of Seedless white, choose the morning or afternoon when the temperature is 15-28 ℃, no wind, no rain, and spray chlormequat 100mg.L with a hand-held pressure watering can -1 For the spikelets that have been separated, but the florets in the spikelets are still crowded together, spray until the agent soaks all the spikes. The front part of the flower spike is blocked by a plastic plate to prevent the chemicals from contacting other branches, leaves and inflorescences. Regular emasculation for pollination at flowering. After 40-50 days, pick the young fruit and put it into a jar, rinse it several times with tap water, add 75% ethanol to infiltrate it for 30 seconds, rinse it once with sterile water, add 2.5% sodium hypochlorite to soak for 5 minutes, and carry out surface sterilization. Rinse with bacterial water 2 times. Under aseptic conditions, the fruit grains were cut open to take out the ovules, and inoculated into the improved ER so...

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Abstract

The invention discloses a method for increasing the breeding efficiency of seedless grape, it is a solution which has dissolved plant growth inhibitor chlormequat or cytokinin 6-BA, spray the solution onto the flower cluster 10-20 days before bloom, obtain the grape fruits 40-50 days after bloom, extracte the ovule to save embryo, the invention improves the embryo developmental rate of Anucleate White from 0-4.4% to 8.3-11.9%; Anucleate Sentianni from 0.5-2.5% to 1.8-21.9%; Anucleate Huoyan from 7.1-17.1% to 14.1-25.4%. The invention improves the embryo developmental rates of these seedless grapes, increases the seedling formation basic numbers of hybrid offspring, thereby obtains more hybrid offspring, enhances the efficiency of breeding, extends the parents choice range of seedless grape cross breeding and accelerats the breeding process of seedless variety.

Description

technical field [0001] The invention belongs to the technical field of plant breeding, and relates to using exogenous plant growth regulating substances and regulating the growth and development process of plant reproductive organ embryos, and combining with embryo rescue technology to promote the acquisition of more hybrid offspring after cross-pollination and improve the fertility Methods for the efficiency of nuclear grape breeding. Background technique [0002] Seedless grapes are popular with consumers both fresh and dried because of their convenience in eating. However, most of the existing seedless grape varieties belong to the Eurasian variety group. These varieties have good flavor and quality, but poor disease resistance. The growing season in many grape growing regions requires the application of many pesticides. Today, the safety of agricultural products is increasingly concerned, and consumers with continuously improved living standards are increasingly demandi...

Claims

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Application Information

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IPC IPC(8): A01H1/02A01H4/00A01N33/12A01P21/00C12N5/04
Inventor 王跃进唐冬梅蔡军社赵荣华
Owner NORTHWEST A & F UNIV
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