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Method for in-vitro rapid breeding of zingiberaceae plant with leaf stems and peduncle resting buds as explants

A technology of explants and crypto buds, applied in the field of agricultural biology, can solve the problems of low induction survival rate, high pollution rate, few sources, etc., and achieves the effects of strong operability, reduced pollution rate, and convenient steps.

Active Publication Date: 2019-09-03
ZHONGKAI UNIV OF AGRI & ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the use of these parts as explants has the problems of few sources, high pollution rate, and low induction survival rate; even if a combination of multiple aseptic treatment methods with complicated operations is used, the sterile induction survival rate is often below 50%.

Method used

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  • Method for in-vitro rapid breeding of zingiberaceae plant with leaf stems and peduncle resting buds as explants
  • Method for in-vitro rapid breeding of zingiberaceae plant with leaf stems and peduncle resting buds as explants
  • Method for in-vitro rapid breeding of zingiberaceae plant with leaf stems and peduncle resting buds as explants

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] The preparation method of embodiment 1MS medium

[0043] The components of MS medium are shown in Table 1. Prepare 1L MS medium: Accurately weigh the various compounds described in Table 1, add appropriate amount of distilled water to dissolve, stir with a glass rod to promote dissolution, adjust the pH to 6.0 with NaOH, and finally set Capacity to 1L.

[0044] Table 1 MS medium components

[0045]

Embodiment 2

[0046] The preparation method of each culture medium of embodiment 2

[0047] 1) Induction medium for promoting cryptobud germination: take 600ml of pure water, add a certain volume of MS mother liquor, 25-35g / L sucrose and 6.5-7.5g / L agar, 3.0-5.0mg / L of BA, 1.0~3.0mg / L TDZ, 0.01~0.02mg / L NAA (BA, TDZ, NAA stock solutions are filtered through filter membrane under sterile conditions), adjust the pH value to 6.0 after distilling to 1 liter, divide After packaging, autoclave, cool to room temperature and store for later use;

[0048] 2) Medium for promoting flakes to induce adventitious buds: take 600ml of pure water, add a certain volume of MS mother liquor, 25-35g / L sucrose and 6.5-7.5g / L agar, 3.0-5.0mg / L of BA, 0.1 ~ 0.3mg / L TDZ (BA, TDZ stock solution is filtered through a filter membrane under sterile conditions), adjust the pH value to 6.0 after the volume is adjusted to 1 liter, autoclave after subpackaging, and store after cooling to room temperature spare;

[0049]...

Embodiment 3

[0051] Example 3 Rapid propagation of 'Hanyue' ginger flower in vitro with leaf-stem cryptobuds as explants.

[0052] (1) Test steps:

[0053] 1) Explant preparation: select the robust leaf stem of 'Hanyue' ginger flower with inflorescence on the top, peel off the leaf sheath layer by layer, and keep the innermost layer of leaf sheath close to the leaf stem. With a hidden bud as the center, the leaf stem is cut into 4-6 cm long stem segments.

[0054] 2) Aseptic treatment of explants and germination induction of cryptobuds: Under aseptic conditions, after soaking in 75% alcohol for 60 seconds, rinse with sterile water for 3 times, and then sterilize with 0.10% mercuric solution for 20 minutes. Rinse with bacteria water 8 times. Cut off both ends of the stem section, keep the leaf stem with the cryptobud as the node, and the upper and lower length is about 0.5 cm, use a blade and tweezers to poke the last leaf sheath to reveal the cryptobud, and inoculate it into the inductio...

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Abstract

The invention discloses a method for in-vitro rapid breeding of zingiberaceae plant with leaf stems and peduncle resting buds as explants. Strong zingiberaceae plant leaf stems with inflorescences areobtained, leaf sheaths are peeled off layer by layer, and the innermost layer of leaf sheaths close to the leaf stems are reserved; the sterilized and processed explants with the resting buds are transplanted into an induction medium for promoting germination of the resting buds, a medium for promoting thin piece induction of adventitious buds, a medium for cluster bud proliferation and a rootingmedium step by step so as to induce germination, proliferation and rooting of the explants; finally, the explants are transplanted into a natural environment. According to the method, it is confirmedthat some zingiberaceae plants have the resting buds for the first time, and an in-vitro cultivation technology with the resting buds as the explants is constructed. The method is easy to operate, the contamination rate of in-vitro cultivation of the zingiberaceae plants is greatly reduced, and the in-vitro rapid propagation efficiency of the zingiberaceae plants is improved.

Description

technical field [0001] The invention belongs to the field of agricultural biotechnology, and relates to a plant tissue culture method, in particular to a method for in vitro rapid breeding of Zingiberaceae plants using leaf stems and cryptic buds of peduncles as explants. Background technique [0002] Zingiberaceae is a large family of monocotyledonous plants. There are about 50 genera and more than 1,300 species of Zingiberaceae plants in the world. There are about 20 genera and 220 species in China, including important edible, medicinal and ornamental plants. , such as ginger, turmeric, ginger flower, grass fruit, sand ginger, galangal, puzzle, etc., have very important economic value. [0003] Zingiberaceae plants are mostly cross-pollinated, and the offspring propagated by seeds cannot maintain the excellent traits of the mother plant, and it takes more than two years from sowing to flowering, so they mainly rely on tubers for reproduction in production. However, this m...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/008A01H4/001A01H4/00
Inventor 胡秀谭嘉川黄嘉琦李晓泳邓莎
Owner ZHONGKAI UNIV OF AGRI & ENG
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