Creation method of switchgrass mutants

A technology of mutants and willow branches, applied in the field of plant breeding, to achieve the effects of easy planting, solving the shortage of materials, and a large base of mutagenic populations

Inactive Publication Date: 2014-11-12
NORTHWEST A & F UNIV
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

However, so far there has been no report on the use of clones as mutagenic materials in the study of switchgrass

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  • Creation method of switchgrass mutants
  • Creation method of switchgrass mutants

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Embodiment Construction

[0041] The present invention will be further described in detail below in conjunction with specific embodiments, which are explanations of the present invention rather than limitations.

[0042] The invention uses the clustered buds of switchgrass as mutagenic materials, and creates mutant plants thereof under the condition of consistent initial genotypes. Specifically follow the steps below:

[0043] Step 1: Take materials and propagate and expand cluster bud populations

[0044] a. In field conditions, when the growth and development of switchgrass plants reached 5-6 nodes, young spikes began to form. Take about 1-1.5 cm of stem node tissue under the ear, and each stem node tissue includes the length of 0.5-0.7 cm above and below the stem node.

[0045] b. Use 70% alcohol to sterilize the surface of the stem node tissue for 1 min, then sterilize with 20% sodium hypochlorite solution for 5-8 min, rinse with sterile water 3-4 times, inoculate into MBP medium, and the MBP med...

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Abstract

The invention discloses a creation method of switchgrass mutants. The creation method comprises the following steps: after a lots of switchgrass multiple shoots are cultured in a tissue culture manner, stripping out individual multiple shoots, cutting off the upper sheaths after acclimatization under natural conditions, performing soaking treatment on the parts 1.5-2cm above the left base parts by use of a solution of ethylmethane sulfonate (EMS) for 3 hours, and after the completion of the soaking treatment, transplanting the multiple shoots of which the sheaths are cut off into a culture medium for culture, and after complete rooting, transplanting to a greenhouse or land, and screening out the mutants according to the phenotypes of the switchgrass. The method is capable of realizing large-scale processing of the mutational original acceptor material and thus increasing the basic number of the variants, and meanwhile, a near-isogenic line material only different from the original acceptor material in target traits can be obtained; and in addition, the mutagenesis process is simple and feasible, the processing cost is relatively low, and the breeding period can be effectively shortened; in short, the method is suitable for creating new materials for the modern biotechnology research of the switchgrass.

Description

technical field [0001] The invention belongs to the technical field of plant breeding and relates to a method for creating a switchgrass mutant. Background technique [0002] Switchgrass (English name Switchgrass, Latin name Panicum virgatum Linn) is a perennial grass C 4 The plant originates from the North American prairie, and has tall plants, simple cultivation, strong resistance to stress, and high annual biomass output. Originally, switchgrass was mainly used as feed crops and garden plants, and it was also used in soil erosion control and other aspects, showing excellent utilization prospects. At the end of the 20th century, the United States took the lead in selecting it as a model bioenergy plant. Since then, various countries have increased their research on the development of switchgrass. The use of modern biotechnology to genetically improve and develop switchgrass has become one of the research hotspots in the field of bioenergy at home and abroad. [0003] Cr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 奚亚军李毛王勇锋
Owner NORTHWEST A & F UNIV
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