8 results about "Panicum virgatum" patented technology

A new, Panicum virgatum plant as described and illustrated, characterized by an upright plant habit, a height of 137 cm, leaf color that starts at 146 B with tips colored 200 B throughout the season, and much of the fall foliage color maturing to 200 C on top of some 146 B.

The invention discloses a method for improving gene editing efficiency of a switchgrass CRISPR / Cas system and application of the method. A CRISPR / Cas9 gene editing vector modified by a promoter and a CRISPR / Cas12i3 (SF01) gene editing vector modified by a Cas core enzyme are subjected to genetic transformation in switchgrass, and research with PvPDS as a target gene finds that the gene editing efficiency of the CRISPR / Cas9 gene editing vector can be improved by replacing the switchgrass endogenous promoters (PvU61, PvU63 and PvUbi) and the Cas core enzyme; and meanwhile, the large fragment deletion proportion in the editing type of the CRISPR / Cas12i3 (SF01) gene editing vector for replacing the Cas core enzyme is also remarkably improved. The method has important significance for optimizing the existing switchgrass gene editing system and expanding the application range of a novel gene editing chassis tool.

The application provides application of PvEIL3a in improving heavy metal stress tolerance of plants, and relates to the technical field of genetic engineering of energy forage plants.In the application, the trait modification of switchgrass is realized by regulating a key gene of a sulfur assimilation pathway, namely, the switchgrass PvEIL3a gene; in the application, the PvEIL3a protein is overexpressed in switchgrass by using a genetic engineering method, and a switchgrass strain with enhanced nutrient transformation efficiency and enhanced heavy metal resistance is obtained, which will provide high-quality materials for the modification and efficient utilization of marginal land and the improvement of heavy metal contaminated land, and has important reference significance for improving the utilization of energy plants.

This invention discloses a gene, PvHsf16, encoding a plant heat shock transcription factor, and its applications. This gene originates from differentially expressed genes upregulated in members of the Hsf gene family of switchgrass under cadmium (Cd) stress. By constructing a yeast expression vector for PvHsf16, PvHsf16 cells exhibited significantly greater cadmium tolerance, indicating that this gene enhances yeast cadmium resistance. PvHsf16 is located in the cell nucleus. Overexpression of the PvHsf16 gene in Arabidopsis thaliana, compared to the wild type under CdCl2 treatment, showed enhanced cadmium tolerance in the PvHsf6 overexpressing lines, with significantly increased chlorophyll content, root length, and biomass. The PvHsf16 gene provides a genetic resource for cultivating switchgrass germplasm suitable for both energy grass and cadmium remediation, and can be applied to the remediation of cadmium-polluted areas, playing an important role in researching phytoremediation technologies and improving soil heavy metal pollution.

A new and distinct plant of ornamental Switch Grass named Panicum ‘October Sky’ with blue-green, upright and arching foliage that red-purple tips and blush in early September. The upright and dense culms produce medium-height plants with airy panicles beginning greyed-green and becoming reddish-purple in September before drying in the fall to tan seed heads that are retained into winter. The new plant is useful in the landscape as a specimen, en masse, or in a container.

The invention relates to the technical field of plant genetic engineering and molecular breeding, and particularly discloses a method for screening stress-resistant interaction genes of switchgrass by using a yeast two-hybrid technology and a breeding method. The method comprises the following steps: firstly, screening candidate proteins interacting with key stress-resistant proteins from switchgrass by using a yeast two-hybrid system, and verifying the specific interaction of the candidate proteins; then, defining a gene combination for verifying interaction as a stress-resistant protein interaction module, connecting all coding sequences in series through 2A self-cleavage peptide, and assembling with a stress inducible promoter to form a single multi-gene expression unit; and finally, introducing the unit into switchgrass through an agrobacterium-mediated method to obtain a transgenic plant with synergistically enhanced stress resistance. According to the method, the transformation from a natural interaction network to rational design breeding is realized, and the efficiency and predictability of stress resistance character improvement are improved.

The invention relates to a switchgrass seedling culture fungicide and a preparation method and application thereof.The switchgrass seedling culture fungicide comprises arbuscular mycorrhizal fungi inoculant, a biochar carrier and an adhesive, the mass-volume ratio of the biochar carrier to the arbuscular mycorrhizal fungi inoculant is 15 g: 15 mL-20 g: 10 mL, the spore concentration of the arbuscular mycorrhizal fungi inoculant is not lower than 30 / mL, and the spore concentration of the biochar carrier is not lower than 20 / mL. The mass of the adhesive is 5%-10% of the mass of the charcoal carrier. The preparation method comprises the following steps: S1, preparing a biochar carrier; s2, mixing the charcoal carrier with the arbuscular mycorrhizal fungus inoculum; s3, preparing the seedling culture fungicide. The application refers to the application of the switchgrass seedling culture fungicide in improving the colonization rate and the infection intensity of the switchgrass seedling root system arbuscular mycorrhizal fungi when switchgrass seedling culture is carried out by adopting saline-alkali soil or a salinization substrate. According to the method, the colonization rate and the infection intensity of the switchgrass root system arbuscular mycorrhizal fungi can be remarkably improved under the condition of saline-alkali stress.