16 results about "Panicum virgatum" patented technology

The invention relates to the technical field of coal-based solid waste resource utilization and mining area ecological restoration, in particular to a coal-based solid waste artificial soil directional pore construction waste dump ecological restoration method which comprises the steps that S1, coal-based solid waste is mixed according to a certain mass ratio; s2, mixing organic matters according to a mass ratio; s3, mixing the mixture obtained in the step S2 with the bacterial liquid; s4, compounding the mixture in the step S3 and the mixture in the step S1; s5, the mixture obtained in the step S4 is mixed with aluminum powder and calcium hydroxide; s6, drying after fermentation in S5, and screening the particle size range to obtain a porous medium; s7, holes are punched in the surface of the waste dump, and then the porous media are buried in the holes; s8, the switchgrass and the apocynum venetum are compounded according to the proportion; s9, planting plants in holes in S7 in a hole sowing manner; and S10, monitoring according to a certain period. The coal-based solid waste resource utilization efficiency and the mining area ecological restoration quality are comprehensively improved through coal-based solid waste particle size and mineral component regulation and control, porous medium pore directional construction and soil-vegetation system coupling.

The application discloses application of a PvFIP37 protein and related biological materials thereof in regulating plant cadmium tolerance. The application clones a PvFIP37 gene from switchgrass and successfully constructs a PvFIP37 overexpression switchgrass. Through analysis of a phenotype of the PvFIP37 overexpression switchgrass under cadmium stress, it is found that, compared with a wild type switchgrass, the increase of the PvFIP37 gene expression amount can enhance the cadmium tolerance of the switchgrass, which indicates that the PvFIP37 has a function of regulating plant cadmium tolerance. The switchgrass PvFIP37 gene provided by the application can be used as an excellent gene resource and is widely applied to the genetic breeding field of the gramineous grasses or other crops, and has important significance for understanding of the plant tolerance under heavy metal stress.

A new, Panicum virgatum plant as described and illustrated, characterized by an upright plant habit, a height of 137 cm, leaf color that starts at 146 B with tips colored 200 B throughout the season, and much of the fall foliage color maturing to 200 C on top of some 146 B.

The application discloses a plant coding gene and protein with improved cadmium resistance and promoted biomass and a transgenic strain. The application carries out RNA-seq sequencing on switchgrass under cadmium stress treatment, screens a differential expression gene with the highest up-regulation fold, and genome annotation shows that the gene codes a member of a heat shock protein 70 family (Hsp70), a chaperone BiP (Binding Protein) protein, and the gene is named as PvBiP2. An overexpression vector of the gene is constructed and is transferred into a cadmium-sensitive yeast mutant strain (ycf1), and the growth condition of the transgenic yeast on a cadmium-containing culture medium is obviously better than that of a control group. Overexpression of the PvBiP2 gene in switchgrass is found to significantly improve the cadmium resistance of the transgenic switchgrass and promote the biomass of the switchgrass.

The invention discloses a method for improving gene editing efficiency of a switchgrass CRISPR / Cas system and application of the method. A CRISPR / Cas9 gene editing vector modified by a promoter and a CRISPR / Cas12i3 (SF01) gene editing vector modified by a Cas core enzyme are subjected to genetic transformation in switchgrass, and research with PvPDS as a target gene finds that the gene editing efficiency of the CRISPR / Cas9 gene editing vector can be improved by replacing the switchgrass endogenous promoters (PvU61, PvU63 and PvUbi) and the Cas core enzyme; and meanwhile, the large fragment deletion proportion in the editing type of the CRISPR / Cas12i3 (SF01) gene editing vector for replacing the Cas core enzyme is also remarkably improved. The method has important significance for optimizing the existing switchgrass gene editing system and expanding the application range of a novel gene editing chassis tool.

The present application belongs to the field of microbial fertilizer, and particularly relates to a microbial fertilizer for promoting growth of switchgrass seedlings and a preparation method thereof. Effective components of the microbial fertilizer are Klebsiella michiganensis, Priestia flexa, Microbacterium yannicii and Leifsonia xyli subsp.Cynodontis, and the preservation numbers of the Klebsiella michiganensis, the Priestia flexa, the Microbacterium yannicii and the Leifsonia xyli subsp.Cynodontis are DSM 25444, DSM 1316, DSM 23203 and DSM 46306 respectively. Under the combined action of the Klebsiella michiganensis, the Priestia flexa, the Microbacterium yannicii and the Leifsonia xyli subsp.Cynodontis, the microbial fertilizer can promote nutrient absorption of the switchgrass seedlings, improve growth of the aboveground part, and improve drought resistance of the switchgrass seedlings. The present application has great potential for improving agricultural production efficiency and realizing sustainable development of agriculture.

This invention provides the application of the PvALS mutant in improving the herbicide resistance of switchgrass, relating to the field of genetic engineering technology. This invention establishes a single-base editing system for switchgrass by infecting callus tissue with Agrobacterium, providing a novel method for gene editing in switchgrass and laying the foundation for functional gene research and breeding work. The PvALS mutant plants obtained by this invention exhibit excellent herbicide resistance, providing a new herbicide-resistant resource for switchgrass, which can be widely planted as an energy crop in various environments.

A new and distinct plant of ornamental Switch Grass named Panicum ‘Half Pint’ with upright blue-green foliage that retains its coloration into the fall. The upright, compact, and dense culms produce small-height plants with broadly airy panicles beginning greyed green, developing rosy-reddish seed heads that are retained as creamy-tan into winter. The upright foliage and inflorescences produce a tight habit that is narrower than the height of the inflorescences. The new plant is useful in the landscape as a specimen, en masse, or in a container.

The application discloses application of a switchgrass PvCOL16 gene in regulation of flowering period of plants. The application obtains a recombinant overexpression vector by using existing plant genetic engineering technology, and then converts rice callus by using an agrobacterium infection conversion method; after tissue culture and identification and screening, a homozygous PvCOL16 transgenic rice plant is obtained. Compared with wild type plants grown under the same conditions, the rice plant overexpressing the PvCOL16 gene shows a late flowering phenotype, delays the flowering of the rice, reduces the plant height, and participates in regulation of expression of genes related to flowering, so that the PvCOL16 gene can be applied to cultivation of late flowering or dwarf varieties, and has high agricultural utilization value.

The application provides application of PvEIL3a in improving heavy metal stress tolerance of plants, and relates to the technical field of genetic engineering of energy forage plants.In the application, the trait modification of switchgrass is realized by regulating a key gene of a sulfur assimilation pathway, namely, the switchgrass PvEIL3a gene; in the application, the PvEIL3a protein is overexpressed in switchgrass by using a genetic engineering method, and a switchgrass strain with enhanced nutrient transformation efficiency and enhanced heavy metal resistance is obtained, which will provide high-quality materials for the modification and efficient utilization of marginal land and the improvement of heavy metal contaminated land, and has important reference significance for improving the utilization of energy plants.

This invention discloses a gene, PvHsf16, encoding a plant heat shock transcription factor, and its applications. This gene originates from differentially expressed genes upregulated in members of the Hsf gene family of switchgrass under cadmium (Cd) stress. By constructing a yeast expression vector for PvHsf16, PvHsf16 cells exhibited significantly greater cadmium tolerance, indicating that this gene enhances yeast cadmium resistance. PvHsf16 is located in the cell nucleus. Overexpression of the PvHsf16 gene in Arabidopsis thaliana, compared to the wild type under CdCl2 treatment, showed enhanced cadmium tolerance in the PvHsf6 overexpressing lines, with significantly increased chlorophyll content, root length, and biomass. The PvHsf16 gene provides a genetic resource for cultivating switchgrass germplasm suitable for both energy grass and cadmium remediation, and can be applied to the remediation of cadmium-polluted areas, playing an important role in researching phytoremediation technologies and improving soil heavy metal pollution.

The invention discloses a switchgrass sowing propagation method, and belongs to the technical field of switchgrass propagation, a composite embedding agent is prepared to embed switchgrass seeds, then low-temperature sand storage stratification treatment is carried out, fluridone is dynamically released through the composite embedding agent, low-temperature treatment is combined to promote switchgrass seed dormancy breaking, and the switchgrass seed dormancy breaking rate is increased. The composite embedding agent can continuously provide an appropriate humidity environment for the seeds after the stratification treatment is completed, so that the secondary dormancy of the seeds is inhibited, the germination rate of the seeds is improved, the sowing and propagation efficiency of switchgrass is improved, and the method has a good application prospect in the field of switchgrass propagation.

A new and distinct plant of ornamental Switch Grass named Panicum ‘October Sky’ with blue-green, upright and arching foliage that red-purple tips and blush in early September. The upright and dense culms produce medium-height plants with airy panicles beginning greyed-green and becoming reddish-purple in September before drying in the fall to tan seed heads that are retained into winter. The new plant is useful in the landscape as a specimen, en masse, or in a container.

The invention relates to the technical field of plant genetic engineering and molecular breeding, and particularly discloses a method for screening stress-resistant interaction genes of switchgrass by using a yeast two-hybrid technology and a breeding method. The method comprises the following steps: firstly, screening candidate proteins interacting with key stress-resistant proteins from switchgrass by using a yeast two-hybrid system, and verifying the specific interaction of the candidate proteins; then, defining a gene combination for verifying interaction as a stress-resistant protein interaction module, connecting all coding sequences in series through 2A self-cleavage peptide, and assembling with a stress inducible promoter to form a single multi-gene expression unit; and finally, introducing the unit into switchgrass through an agrobacterium-mediated method to obtain a transgenic plant with synergistically enhanced stress resistance. According to the method, the transformation from a natural interaction network to rational design breeding is realized, and the efficiency and predictability of stress resistance character improvement are improved.

The invention relates to a switchgrass seedling culture fungicide and a preparation method and application thereof.The switchgrass seedling culture fungicide comprises arbuscular mycorrhizal fungi inoculant, a biochar carrier and an adhesive, the mass-volume ratio of the biochar carrier to the arbuscular mycorrhizal fungi inoculant is 15 g: 15 mL-20 g: 10 mL, the spore concentration of the arbuscular mycorrhizal fungi inoculant is not lower than 30 / mL, and the spore concentration of the biochar carrier is not lower than 20 / mL. The mass of the adhesive is 5%-10% of the mass of the charcoal carrier. The preparation method comprises the following steps: S1, preparing a biochar carrier; s2, mixing the charcoal carrier with the arbuscular mycorrhizal fungus inoculum; s3, preparing the seedling culture fungicide. The application refers to the application of the switchgrass seedling culture fungicide in improving the colonization rate and the infection intensity of the switchgrass seedling root system arbuscular mycorrhizal fungi when switchgrass seedling culture is carried out by adopting saline-alkali soil or a salinization substrate. According to the method, the colonization rate and the infection intensity of the switchgrass root system arbuscular mycorrhizal fungi can be remarkably improved under the condition of saline-alkali stress.

A new and distinct plant of ornamental Hybrid Switch Grass named Panicum ‘Switcheroo’ with narrow upright foliage with variegated yellowish margins. The upright and dense culms produce compact plants that do not lodge. The dense columnar panicles beginning yellowish-chartreuse and becoming tan seed heads that are retained into winter. The new plant is useful in the landscape as a specimen, en masse, or in a container.