52 results about "Panicum virgatum" patented technology

Methods for increasing the product yield from plants, preferably transgenic plants, are provided. It has been discovered that in vitro cultures from donor plants produce plants that have two or three fold increase in product yield. One embodiment provides a method for increasing product yield from a transgenic plant by initiating an in vitro culture from a donor transgenic plant, wherein the donor transgenic plant is genetically engineered to produce a product and regenerating a second transgenic plant from the in vitro culture, wherein the yield of the product from the second transgenic plant is greater than the yield of the product from the donor transgenic plant. In a preferred embodiment, the transgenic plant is a graminaceous plant such as switchgrass.

A process for converting lignocellulosic materials which are field residues such as cotton stalks and corn stover, process residues such as sugarcane bagasse and sweet sorghum bagasse, woody parts of energy crops such as switchgrass and miscanthus, forest residues or byproducts of the wood processing industries such as sawdust from sawmills to a liquid biofuel by a series of processing steps wherein the feed materials are hydrolysed in three stages and withdrawn as three product streams each consisting of solubilized fragments of one of the three major components of the feed materials and a set of concurrently operating processing steps wherein each of the three product streams is transformed through chemical or biochemical processes into products, such as pure lignin and ethanol, that have a high calorific value and process wherein these products with high calorific value are combined to form a liquid biofuel.

Biomass may become an important source of combustible fuel in the future. For biomass, such as switchgrass, to be economically viable as a fuel, the processes required to take the plant material from a green, growing plant in the field to an energy dense package must be efficient. A self-propelled bale picking truck simplifies and speeds bale collecting, stacking, and staging for loading onto a truck for transport over the road.

The invention relates to the field of genetic engineering, in particular to plant drought-resistant relevant protein PvSnRK 2.3 and an encoding gene and application thereof. The drought-resistant relevant protein PvSnRK 2.3 is from panicum virgatum, and an amino acid sequence of the drought-resistant relevant protein PvSnRK 2.3 is shown as SEQ ID NO. 1. The drought-resistant relevant protein and the encoding gene thereof have the important theoretical and practical significance for improving and enhancing the stress resistance of arabidopsis, improving yield, accelerating the anti-stress molecular breeding progress and saving water resources effectively.

Biomass may become an important source of combustible fuel in the future. For biomass, such as switchgrass, to be economically viable as a fuel, the processes required to take the plant material from a green, growing plant in the field to an energy dense package must be efficient. A self-propelled bale picking truck simplifies and speeds bale collecting, stacking, and staging for loading onto a truck for transport over the road.

The invention discloses a tissue culture method of monocotyledons. The method relates to a method for obtaining an II type callus of the monocotyledons, comprising the following steps: 1, with mature seeds of monocotyledons as explants, inoculating the explants on a callus-inducing culture medium, induction-culturing for 4-6 weeks at temperature of 25+ / -2 DEG C under the condition of light resistance to obtain a callus 1; 2, peeling a white callus at the outermost layer from the callus 1 obtained from the step 1, collecting an inside yellow embryonic callus, inoculating the inside yellow embryonic callus on a callus subculture medium, culturing for 3 weeks at the temperature of 25+ / -2 DEG C under the condition of light resistance to obtain a callus 2; and 3, selecting a callus the volume of which is increased by above 60 percent within 3 weeks of subculturing from the callus 2 obtained from the step 2, namely, the II type callus. Experiments prove that the tissue culture method is suitable for switchgrasses of different ecotypes, and is capable of obtaining an excellent callus with high regeneration efficiency within short time, thus the time and cost are saved, and the biological breeding process of switchgrass genetic modification is accelerated.

The invention relates to a rapid seedling raising method of switchgrass tissue culture and particularly relates to a tissue culture technology of a biological energy plant switchgrass. The method disclosed by the invention is carried out according to following process steps of: treating seeds, inducing calluses and buds, and carrying out rooting culture. According to the rapid seedling raising method disclosed by the invention, a switchgrass seed tissue culture method is adopted, so that good varieties can be reproduced, excellent seedlings can be selected from seed seedlings to be used for a genetic transformation experiment, and a lot of money and energy are prevented from spending. The method disclosed by the invention is feasible, is simple to operate, and provides an effective way for carrying out crossbreeding and genetic transformation experiments by people.

Biomass may become an important source of combustible fuel in the future. For biomass, such as switchgrass, to be economically viable as a fuel, the processes required to take the plant material from a green, growing plant in the field to an energy dense package must be efficient. A bale destacker may be used to disassemble stacks of bales of biomass after the bales have been brought from the field and before the bales are used for fuel. The bale destacker disassembles a stack of bales with no manual labor. A conveyor is used to move bales, first in stacks, then in pairs to and from the bale destacker.

The invention relates to a method for building panicum virgatum into vegetation in severe saline-alkali soil. The method comprises the steps as follows: soil preparation, transplanting hole digging, base fertilizer application, treating agent spraying, seedling selection and preparation, digging, transplanting and plant division, field transplanting, field management and the like. According to the method, a field planting experiment is performed in the severe saline-alkali soil in the yellow river delta region, the survival rate is high, the vegetation building speed is high, the vegetation can be built in the same year, and a considerable number of grasses and seeds are harvested; the energy grass, namely, panicum virgatum is successfully planted in the severe saline-alkali soil, the method can play an active role in promoting development and utilization of the severe saline-alkali soil and popularization of biomass energy, thereby having a broad application prospect.

The invention discloses a Panicum virgatum planting method and an application of generating electricity as bio-energy, which belongs to the technology of plant cultivation by bio-energy generating electricity and an application thereof. The invention provides a scientific cultivation method which is easily grasped and has high yield, namely, the scientific cultivation means of seed selection, seed taking, breeding, planting, and the like. After being harvested, reaped, compressed and bundled, the Panicum virgatum can be directly used as bio-energy for generating electricity. The Panicum virgatum of the invention can be a grass seed with high yield to be a bushy underbrush by high-density cultivation and further improve the yield. Simultaneously, the technology of the invention can also be applied to planting in sand and sloping field and is a scientific cultivation method which is easily grasped. Therefore, the Panicum virgatum planted by the technology of the invention can be a new bio-energy for generating electricity.

Transgenic plants, plant material, and plant cells for synthesis of polyhydroxyalkanoates, preferably poly(3-hydroxybutyrate) (also referred to a as PHB) are provided. Preferred plants that can be genetically engineered to produce PHB include plants that do not normally produce storage products such as oils and carbohydrates, and plants that have a C4 NAD-malic enzyme photosynthetic pathway. Such plants also advantageously produce lignocellulosic biomass that can be converted into biofuels. An exemplary plant that can be genetically engineered to produce PHB and produce lignocellulosic biomass is switchgrass, Panicum virgatum L. A preferred cultivar of switchgrass is Alamo. Other suitable cultivars of switchgrass include but are not limited to Blackwell, Kanlow, Nebraska 28, Pathfinder, Cave-in-Rock, Shelter and Trailblazer.

The invention discloses a production method of biogas, belonging to the technical field of biomass energy production. The biogas is prepared from the following raw materials in parts by weight: 30-35 parts of panicum virgatum, 40-45 parts of sorghum stalks, 45-50 parts of animal dung, 15-25 parts of biogas residues and 10-20 parts of biological bacteria. The production method comprises the following steps of respectively crushing the panicum virgatum and the sorghum stalks, adding water to regulate the concentration to 20%, and then, acidizing at the constant temperature of 30-40 DEG C for 5 days to obtain an acidized solution; adding the animal dung, the biogas residues and the biological bacteria into the acidized solution, uniformly mixing all the components, and fermenting at the constant temperature of 50-60 DEG C; and collecting gases generated in the constant-temperature fermentation process. Compared with the existing biogas production technology, the production method disclosed by the invention has the advantages that the biogas production lasting time is longer, and the biogas yield is higher.

The invention discloses application of switchgrass in desert control. Microsclerotia and mycelia adhering to the surfaces of seeds can be killed by adopting carbendazim spray; after seed dressing processing is performed with plant nutrients for herbal Chinese medicines, the seed germination rate and the plant weight and chlorophyll content of seedlings are improved, and therefore the yield of the swichgrass is improved; through the reasonable usage of a seed coating agent, pest and disease damage can be controlled on the condition that pesticide is not used, therefore, the switchgrass can be survived in desert and return green in the next year, plants on the ground can grow to 2 meters, and the underground roots can grow to 2 meters to well fix sand. The desert is controlled by adopting a sowing mode, labor input is reduced, and the effect that the switchgrass is planted and survived in the same year never achieves before; meanwhile, the switchgrass is good forage grass and is good feed of cows, horses and sheep and can continuously grow about 10 years.

The invention discloses a genetic transformation method for panicum virgatum L., and belongs to the technical field of plant bioengineering. The method comprises disinfection of mature panicum virgatum L. seeds, induction and propagation expanding of type II high-quality embryonic callus, kanamycin screening to obtain kanamycin-resistant callus tissue after agrobacterium infection, kanamycin-resistant callus tissue regeneration and rooting, and finally acquisition of positive transgenic panicum virgatum L. plants. The method is mainly used for solving the problems of large-scale screening and commercialized production of transgenic panicum virgatum L.. Experimental data show that by the adoption of the method, low-cost kanamycin can be used as a transgenic plant resistance screening reagent, the positive transgenic panicum virgatum L. plants are successfully obtained in 4-5 months, genetic transformation efficiency reaches up to 60%, 100 genetic vectors can be transformed by each person every year, and the working efficiency of producing more than 2,000 independent positive transgenic panicum virgatum L. plants is achieved.

The invention discloses application of a panicum virgatum L. S-adenosylmethionine synthase gene SAMS1 to change of the lignin content and further improvement on the saccharification efficiency, and belongs to the technical field of plant genetic engineering. The application comprises the following main contents: performing cloning and sequence determination on the panicum virgatum L. SAMS1 gene; constructing an interference expression vector (pANIC8B-PvSAMS1-RNAi) of the PvSAMS1 gene; transforming a panicum virgatum L. callus by an agrobacterium mediated method, and transforming an interference fragment of a target gene into lowland-type Alamo wild-type panicum virgatum L. to obtain a transgenic plant with reduced total lignin content. The expression quantity of the PvSAMS1 in the obtainedtransgenic plant is significantly reduced, the lignin content is significantly reduced, and the saccharification efficiency is significantly improved. Through regulation and control of lignin metabolism by the identified panicum virgatum L. SAMS1, a novel target can be provided for molecular breeding in future.

The invention discloses a microbial inoculum for producing cellulase and hemicellulase by a natural lignocellulose material. The microbial inoculum for producing the cellulase and the hemicellulase comprises the following active ingredients: Achromobacter xylosoxidans, Alcaligenes faecalis, Fusarium sporotrichioides and Fusarium poae. In the invention, microbes in the microbial inoculum for producing the cellulase and the hemicellulase have a strong synergistic effect so that a stable ecological system can be formed in an autologous mode. The microbial inoculum is especially developed for an energy crop-Panicum virgatum but is not limited to production of the cellulase and the hemicellulase by use of the Panicum virgatum, and meanwhile the inoculum can be also used for producing the cellulase and the hemicellulase by waste crop straw.

The invention provides a method for cultivating octoploid lowland type switchgrass. The method comprises the following steps: (1) induction of tissue culture; (2) micropropagation of the tissue culture; (3) chromosome doubling induction; (4) bud differentiation induction; (5) rooting induction; (6) transplanting; and (7) ploidy identification. The young panicle of the switchgrass serves as an explant to induce the tissue culture, so that the regeneration rate of the young panicle is high; colchicine is used for carrying out artificial chromosome doubling treatment, the doubling effect of the colchicine is stable, and chimera plants are few; and ploidy identification is carried out after the induction of germination and rooting so as to guarantee that all screened plants are the octoploid lowland type switchgrass, and a foundation is laid for future selection of varieties of the octoploid lowland type switchgrass.

The invention relates to a panicum virgatum and wild soybean mixed seeding technology for saline-alkali soil. The panicum virgatum and wild soybean mixed seeding technology for the saline-alkali soil has the advantages that by means of seed pretreatment, scientific and reasonable mixed seeding, intermission seeding and the like, seed germination and growth are promoted effectively, and high survival rate, strong stress resistance, high product yield, high quality and the like are achieved; panicum virgatum is harvested from the end of the July to the beginning of the August, thereby being higher in palatability; fresh panicum virgatum yield increases by 10% and reaches 500-3800 kg / mu, and crude protein content increases from 7% to 10%; the panicum virgatum and wild soybean which are subjected to mixed seeding are synergistic and capable of promoting mutual growth, high stability is achieved, high-quality forage grass is provided for development of the animal husbandry of China, and the technology plays an active driving role in development and utilization of the saline-alkali soil and biomass energy popularization.

The invention discloses a full-length cDNA (complementary Deoxyribose Nucleic Acid) of a switchgrass lignin biosynthetic enzyme gene PvCCoAOMT. The full-length cDNA has a nucleotide sequence as shown in (1) or (2): (1) a nucleotide sequence shown as SEQ ID No.1, and (2) a nucleotide sequence with the same function and formed by replacing, missing or adding one or more nucleotide to the nucleotide sequence as shown in SEQ ID No.1. The invention also provides a cloning method of the full-length cDNA of the switchgrass lignin biosynthetic enzyme gene PvCCoAOMT. The cloning method provides convenience for the subsequent study on primer design, vector construction and genetic transformation by utilizing the sequence of the full-length cDNA of the switchgrass lignin biosynthetic enzyme gene PvCCoAOMT, and also provides the foundation for developing the study on fluorescence quantitative expression, transgenosis, gene function identification and the like in switchgrass in future.

The invention discloses a jujube tree planting controlled-release fertilizer prepared from the following raw materials in parts by weight: 20-28 parts of brown sugar residues, 40-50 parts of switchgrass, 26-30 parts of nereocystis, 50-60 parts of waste woods, 18-25 parts of slaked lime, 20-24 parts of tea leaf residues, 60-70 parts of castor seed cakes, 2-3 parts of a biological fermentation microbial agent, 12-15 parts of limestone flour, 4-5 parts of cupric sulfate pentahydrate, 8-12 parts of ammonium chloride, 10-12 parts of potassium nitrate, 7-9 parts of urea, 2-3 parts of EDTA chelated zinc, 6-8 parts of a phosphorus fertilizer, 8-12 parts of alkyd resin, 2-3 parts of isopropanol, 3-4 parts of sodium dodecylbenzene sulfonate, 4-5 parts of a crosslinking agent and an appropriate amount of water. According to the preparation method disclosed by the invention, the characteristic of slow degradation of resin after solidification is used to ensure that N, P and K are effectively fused in the resin; the rate of degradation can be controlled by controlling the concentrations of the resin and the crosslinking agent to achieve the aim of release control; and a small amount of slaked lime which is particularly added has functions of changing soil and sterilizing, thereby providing alkaline soil suitable for growing jujube trees.

Switchgrass is an increasingly important biofuel crop, but knowledge of switchgrass fungal pathogens is not extensive. The purpose of this research was to identify the fungal pathogens that decrease crop yield of switchgrass grown in Tennessee and to investigate a potential sustainable disease management strategy from a value-added by-product of the switchgrass biofuel conversion process. The specific objectives were 1) to identify and characterize prevalent fungal pathogens of switchgrass in Tennessee, 2) assess switchgrass seed produced in the United States for seedborne fungal pathogens, and 3) evaluate switchgrass extractives for antimicrobial activity against plant pathogens.

The invention belongs to the technical field of ecological agriculture and particularly relates to a mixed seeding method for switchgrass and wild soybeans in saline-alkali land. The mixed seeding method is achieved through the following steps that firstly, after the saline-alkali land is ploughed and raked to be flat, mixed fertilizer composed of farmyard manure and erythromycin waste residues is applied to the saline-alkali land, and ridging is performed; secondly, switchgrass seeds and wild soybean seeds are placed in a seed processing agent in advance to be soaked for 8-10 min, hole sowing is performed on ridges, the wild soybean seeds are sown first, the switchgrass seeds are sown after soil covering, and soil covering and filling are performed so that the saline-alkali land can be flat; thirdly, normal field management is performed after sowing. According to the method, the switchgrass seeds and the wild soybean seeds are wrapped through the seed processing agent so that the seeds can be prevented from being eroded; meanwhile, germination of the seeds is accelerated, the germination rate and the germination potential are increased, the seeds are good in quality, operation is easy and fast, a large amount of manpower and a larger number of material resources are saved, the two crops cooperate with each other, growth is mutually promoted, stability is good, and high-quality forage grass is provided for development of animal husbandry in China.

The invention provides cat litter products and comprises the manufacture of a granulated panicum virgatum component which is mainly mixed with a granulated biochar component. Each component is granulated via an extrusion technique to form particles of about 40 microns. The combination of panicum virgatum and biochar granulated sand products together provides non-toxic and naturally safe, biodegradable, deodorant, and scented antibacterial sands; and the antibacterial sands are convenient for handling of convenient size agglomeration, have very little dust, and reduce track of cats.

The invention relates to application of a switchgrass SBP-box type transcription factor PvSPL6 and a recombinant vector of the switchgrass SBP-box type transcription factor PvSPL6, and belongs to the technical field of plant genetic engineering, and the switchgrass SBP-box type transcription factor PvSPL6 can regulate and control the flowering time, the stem node length and the biomass yield of switchgrass. The invention also provides a recombinant vector which is used for overexpression of pANIC6B-PvSPL6 or inhibition expression of pANIC8B-PvSPL6-RNAi, and after the recombinant vector pANIC6B-PvSPL6 and the pANIC8B-PvSPL6-RNAi are transferred into switchgrass, the blooming time, the stem node length and the biomass yield of the switchgrass are all influenced. Compared with a wild type, the flowering time of the obtained transgenic plant (PvSPL6-RNAi) with PvSPL6 transcript inhibition expression is delayed by about 40 days, the stem node length is increased by about 50%, and the biomass is increased by 63%.

Novel strains of Pseudomonas fluorescens are disclosed. Several novel mutated strains of Pseudomonads are engineered by repetitive culturing of a parent strain under stressed conditions. Various enriched populations are screened and ranked based on relative performance indices including viable cell yield during growth, efficacy in suppression of dry rot disease, cell growth recovery after dry storage and resistance to switchgrass hydrolysate.

The invention belongs to the technical field of ecological agriculture and particularly relates to a mixed seeding method for switchgrass and wild soybeans in saline-alkali land. The mixed seeding method is achieved through the following steps that firstly, after the saline-alkali land is ploughed and raked to be flat, mixed fertilizer composed of farmyard manure and erythromycin waste residues is applied to the saline-alkali land, and ridging is performed; secondly, switchgrass seeds and wild soybean seeds are placed in a seed processing agent in advance to be soaked for 8-10 min, hole sowing is performed on ridges, the wild soybean seeds are sown first, the switchgrass seeds are sown after soil covering, and soil covering and filling are performed so that the saline-alkali land can be flat; thirdly, normal field management is performed after sowing. According to the method, the switchgrass seeds and the wild soybean seeds are wrapped through the seed processing agent so that the seeds can be prevented from being eroded; meanwhile, germination of the seeds is accelerated, the germination rate and the germination potential are increased, the seeds are good in quality, operation is easy and fast, a large amount of manpower and a larger number of material resources are saved, the two crops cooperate with each other, growth is mutually promoted, stability is good, and high-quality forage grass is provided for development of animal husbandry in China.

The invention discloses a heavy metal remediation agent formula for soil remediation, which comprises the following raw materials: deseeded sunflower heads, switchgrass branches and leaves, kaolin, calcium carbonate, an organic fertilizer, pinus elliottii needle leaves, polyaluminum chloride, polyaluminum ferric chloride, bacterial strains and a proper amount of water. Through addition of an organic fertilizer, a large amount of organic matter and nutritional ingredients can be supplied to the contaminated soil, the potential fertility of the contaminated soil is improved, the buffer performance of the soil is improved through modified calcination of kaolin in combination with calcium carbonate, the soil is loosened, and by adding polyaluminum chloride and polyaluminum ferric chloride, theactivity of heavy metal in the soil and the effectiveness of plants are improved; the utilization value of the soil is greatly increased, and the soil remediation is safe and effective through the addition of strains. The preparation method of a formula of a heavy metal repairing agent for soil remediation is convenient to operate, environmentally friendly and harmless, and the prepared heavy metal repairing agent is low in cost, obvious in effect and suitable for wide application and popularization.

The invention relates to a method for isolating efficiently protoplast from switchgrass leaves, belonging to the technical filed of suspension cell lines. In the method, switchgrass leaves in different periods are selected, and the steps mainly comprise cleaning leave surfaces through wiping with 75% alcohol, cutting the leaves into filaments about 5mm width, putting the filaments into a beaker, keeping the filaments in a pre-plasmolysis liquid standing for 1 hour under dark conditions, adding an enzyme solution, carrying out a low-speed enzymatic hydrolysis for 2 hours, keeping standing for 12 hours, split-charging after-separating liquid into 1.5 ml centrifuge tubes to carry out a centrifugation in 800 rpm for 10 min after which supernatant is abandoned, and using a buffer solution for washing for three times. Obtained lower deposition is dissociated somatic cell protoplast. The method of the invention has advantages of simple and easy operation, and low requirements for equipment and culturing conditions.

The invention relates to materials and methods useful for controlling the unwanted spread of energy crop plants. The methods involve an F1 hybrid transgenic switchgrass plant containing a transgene causing seed infertility. The methods also involve one or more transcription factors that activate expression of the transgene. Such F1 hybrid plants are incapable of forming viable seeds.

The invention provides cDNA of a switchgrass tonoplast Na<+> / H<+> antiporter protein gene PvNHX1, and is characterized in that the cDNA has a nucleotide sequence shown in SEQ ID No.1. The invention also provides a cloning method of the cDNA of the switchgrass tonoplast Na<+> / H<+> antiporter protein gene PvNHX1 according to the claim 1, wherein the method comprises the specific steps: (1) extracting total RNA of switchgrass, and carrying out reverse transcription into cDNA; (2) taking the cDNA as a template, and carrying out a high-fidelity full-length PCR reaction by a PvNHX1 gene full-length specific primer; and (3) recovering and purifying the PCR product of the step (2), carrying out an A addition reaction, and thus obtaining the cDNA of the switchgrass tonoplast Na<+> / H<+> antiporter protein gene PvNHX1. The method for obtaining the switchgrass PvNHX1 gene full-length cDNA sequence lays a foundation for vector construction, genetic transformation and the like by utilizing the sequence later on.