Rapid seedling raising method of switchgrass tissue culture
A technology of tissue culture and willow branch, which is applied in the field of plant tissue culture, to achieve the effect of avoiding money and energy, the method is feasible, and the operation is simple
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Embodiment 1
[0033] In the invention, switchgrass seeds are selected as explants, and different hormone ratios are added to MS medium to induce callus, differentiation and rooting induction. The specific production process is:
[0034] Pick explants
[0035] Choose 100 mature and plump switchgrass seeds (0.225 g).
[0036] Callus induction and shoot differentiation
[0037] The seeds were placed in a Petri dish, and 10 ml of 6% (v / v) NaClO was used for surface disinfection for 2 h, during which they were placed on a shaker and kept stirring;
[0038] Discard the sodium hypochlorite, wash it, and soak it in distilled water overnight;
[0039] Disinfect with 10 ml of 6% (v / v) NaClO for 20 min, and put it into the culture medium under sterile conditions. The medium formula is
[0040] MS basal medium was supplemented with 2,4-D 5 mg / L, 6-BA 1.2 mg / L, NAA 1.0 mg / L, and the pH was 5.8.
[0041] At 24°C, cultivate in a light incubator for 10-15 days to induce callus tissue, and when yo...
Embodiment 2
[0049] In the invention, switchgrass seeds are selected as explants, and different hormone ratios are added to MS medium to induce callus, differentiation and rooting induction. The specific production process is:
[0050] (1) Selection of explants
[0051] Choose 100 mature and plump switchgrass seeds (0.225 g).
[0052] (2) Callus induction and bud differentiation
[0053] Place the seeds in a petri dish, sterilize the surface with 5 ml of 6% (v / v) NaClO for 2 h, and keep stirring on a shaker during this period;
[0054] Discard the sodium hypochlorite, wash it, and soak it in distilled water overnight;
[0055] Disinfect with 5 ml of 6% (v / v) sodium NaClO for 20 min, and put it into the culture medium under sterile conditions. The medium formula is
[0056] MS basal medium was supplemented with 2,4-D 6 mg / L, 6-BA 1.5 mg / L, NAA 1.2 mg / L, and the pH was 5.8.
[0057] At 24°C, culture in a light incubator for 15-20 days to induce callus tissue, differentiate and cultur...
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