Rapid seedling raising method of switchgrass tissue culture

A technology of tissue culture and willow branch, which is applied in the field of plant tissue culture, to achieve the effect of avoiding money and energy, the method is feasible, and the operation is simple

Inactive Publication Date: 2013-08-14
JIANGSU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, similar to tissue culture in most grasses, switchgrass has many difficulties in artificially inducing callus

Method used

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  • Rapid seedling raising method of switchgrass tissue culture
  • Rapid seedling raising method of switchgrass tissue culture
  • Rapid seedling raising method of switchgrass tissue culture

Examples

Experimental program
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Effect test

Embodiment 1

[0033] In the invention, switchgrass seeds are selected as explants, and different hormone ratios are added to MS medium to induce callus, differentiation and rooting induction. The specific production process is:

[0034] Pick explants

[0035] Choose 100 mature and plump switchgrass seeds (0.225 g).

[0036] Callus induction and shoot differentiation

[0037] The seeds were placed in a Petri dish, and 10 ml of 6% (v / v) NaClO was used for surface disinfection for 2 h, during which they were placed on a shaker and kept stirring;

[0038] Discard the sodium hypochlorite, wash it, and soak it in distilled water overnight;

[0039] Disinfect with 10 ml of 6% (v / v) NaClO for 20 min, and put it into the culture medium under sterile conditions. The medium formula is

[0040] MS basal medium was supplemented with 2,4-D 5 mg / L, 6-BA 1.2 mg / L, NAA 1.0 mg / L, and the pH was 5.8.

[0041] At 24°C, cultivate in a light incubator for 10-15 days to induce callus tissue, and when yo...

Embodiment 2

[0049] In the invention, switchgrass seeds are selected as explants, and different hormone ratios are added to MS medium to induce callus, differentiation and rooting induction. The specific production process is:

[0050] (1) Selection of explants

[0051] Choose 100 mature and plump switchgrass seeds (0.225 g).

[0052] (2) Callus induction and bud differentiation

[0053] Place the seeds in a petri dish, sterilize the surface with 5 ml of 6% (v / v) NaClO for 2 h, and keep stirring on a shaker during this period;

[0054] Discard the sodium hypochlorite, wash it, and soak it in distilled water overnight;

[0055] Disinfect with 5 ml of 6% (v / v) sodium NaClO for 20 min, and put it into the culture medium under sterile conditions. The medium formula is

[0056] MS basal medium was supplemented with 2,4-D 6 mg / L, 6-BA 1.5 mg / L, NAA 1.2 mg / L, and the pH was 5.8.

[0057] At 24°C, culture in a light incubator for 15-20 days to induce callus tissue, differentiate and cultur...

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Abstract

The invention relates to a rapid seedling raising method of switchgrass tissue culture and particularly relates to a tissue culture technology of a biological energy plant switchgrass. The method disclosed by the invention is carried out according to following process steps of: treating seeds, inducing calluses and buds, and carrying out rooting culture. According to the rapid seedling raising method disclosed by the invention, a switchgrass seed tissue culture method is adopted, so that good varieties can be reproduced, excellent seedlings can be selected from seed seedlings to be used for a genetic transformation experiment, and a lot of money and energy are prevented from spending. The method disclosed by the invention is feasible, is simple to operate, and provides an effective way for carrying out crossbreeding and genetic transformation experiments by people.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture, in particular to the tissue culture technology of bioenergy plant switchgrass. Background technique [0002] Switchgrass (English name Switchgrass, Latin name Panicum virgatumlinn ), also known as "energy grass", because of its simple cultivation techniques, wide adaptability, strong ability to prevent wind and sand fixation, tolerance to barrenness, and high biological yield, it has been identified as the preferred bioenergy plant for the production of dye ethanol internationally, and a large amount of Human and material resources for research and development. [0003] Using transgenic technology to genetically improve energy plants is a new breakthrough in the application of modern genetic engineering technology in the field of agriculture. As an important new bioenergy plant, switchgrass has become one of the main targets of gene transformation. Among them, the use of transgeni...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00A01C1/00
Inventor 杨冉黄萍杜道林杨淞惠祁珊珊
Owner JIANGSU UNIV
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