Method for cultivating octoploid lowland type switchgrass

An octoploid and willow branch technology, applied in the field of plant breeding, can solve the problems of limited germplasm resources and single planting varieties, and achieve the effects of fewer chimera plants, mature methods, and stable doubling effects

Inactive Publication Date: 2016-02-24
BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, as an introduced plant, switchgrass has very limited germplasm resources in China, and the planting varieties are relatively single. Therefore, it is particularly important to carry out germplasm innovation and variety selection of switchgrass on the basis of existing resources.

Method used

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  • Method for cultivating octoploid lowland type switchgrass
  • Method for cultivating octoploid lowland type switchgrass
  • Method for cultivating octoploid lowland type switchgrass

Examples

Experimental program
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Effect test

Embodiment 1

[0036] Taking the tetraploid lowland switchgrass variety Alamo as the research object, at the booting stage, cut the immature young ears of switchgrass with bracts that grew robustly, stripped the outer bracts and leaf sheaths, and cut the young ears with inner bracts into 1.8 -2.2 cm segments to obtain young ear segments containing inner bracts. On the ultra-clean workbench, first disinfect the surface with 75% ethanol by volume for 30 seconds, rinse with sterile distilled water twice, then sterilize with 20% NaClO by volume for 10 minutes, and finally use sterile distilled water Wash 3-4 times, place on sterilized filter paper, and blot the surface moisture.

[0037] 1): Cut the sterilized young ear section containing the inner bract lengthwise into two halves, and then inoculate it in induction medium (MS basic medium + 3% maltose + 5mg / L2, 4-D + 1.2mg / L6 -BA+0.8% plant gel, pH value 5.8), induce callus tissue under dark conditions at 25±2°C, subculture after 10 days, subc...

Embodiment 2

[0048] Taking the tetraploid lowland switchgrass variety Kanlow as the research object, at the booting stage, cut the immature young ears of switchgrass with bracts growing vigorously, stripped the outer bracts and leaf sheaths, and cut the young ears with inner bracts into 1.8 -2.2 cm segments to obtain young ear segments containing inner bracts. On the ultra-clean workbench, first disinfect the surface with 75% ethanol by volume for 30 seconds, rinse with sterile distilled water twice, then sterilize with 20% NaClO by volume for 10 minutes, and finally use sterile distilled water Wash 3-4 times, place on sterilized filter paper, and blot the surface moisture.

[0049] 1): Cut the sterilized young ear section containing the inner bract lengthwise into two halves, and then inoculate it in induction medium (MS basic medium + 3% maltose + 5mg / L2, 4-D + 1.2mg / L6 -BA+0.8% plant gel, pH value 5.8), induce callus tissue under dark conditions at 25±2°C, subculture after 10 days, sub...

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Abstract

The invention provides a method for cultivating octoploid lowland type switchgrass. The method comprises the following steps: (1) induction of tissue culture; (2) micropropagation of the tissue culture; (3) chromosome doubling induction; (4) bud differentiation induction; (5) rooting induction; (6) transplanting; and (7) ploidy identification. The young panicle of the switchgrass serves as an explant to induce the tissue culture, so that the regeneration rate of the young panicle is high; colchicine is used for carrying out artificial chromosome doubling treatment, the doubling effect of the colchicine is stable, and chimera plants are few; and ploidy identification is carried out after the induction of germination and rooting so as to guarantee that all screened plants are the octoploid lowland type switchgrass, and a foundation is laid for future selection of varieties of the octoploid lowland type switchgrass.

Description

technical field [0001] The invention belongs to the field of plant breeding, and in particular relates to a method for cultivating octoploid lowland switchgrass. Background technique [0002] Switchgrass (Panicumvrigatum L.), the Poaceae Panicum, is a perennial herbaceous C4 plant, native to the prairie east of the Rocky Mountains in North America and south of 55° north latitude. It has tall plants and developed root systems, and is usually used for grazing, soil and water conservation, Ecological construction and landscaping, etc. Because of its strong adaptability, high yield potential, strong drought and ridge tolerance, and high lignocellulose content, switchgrass is one of the energy crops with the most development potential, and it is used as a model for the development of cellulosic ethanol in the United States Energy plants are commercially developed and utilized. [0003] Switchgrass was introduced into my country in the 1980s, and many years of experimental resea...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/005
Inventor 岳跃森范希峰武菊英侯新村朱毅赵春桥张爽
Owner BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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