The invention discloses a direct feeding type biological fermentation feed additive and a preparation method and an application thereof. The feed additive provided by the invention is composed of ingredients, fermentation probiotics, enzymic preparations and saline solution medium. The product can be directly applied to livestock breeding and aquaculture, and has the effects of improving immunity of animals, enhancing premonition, improving food consumption and a feed conversion ratio, treating and preventing enteric diseases of animals, adjusting balance of intestinal microecology of animals and resisting stress. According to the method provided by the invention, the production strains are screened in a combined manner, a purification method is completed, production time of the strains is subjected to strict quality control, and the problem that biological fermentation is instable is solved. The feed additive is directly used after fermentation, the condition that no damage is caused to all bioactivators generated after fermentation is ensured, and meanwhile the problems about transportation and bacterial contamination in storage are also avoided, so that the effect and security of the product are improved, the cultivation cost is greatly reduced, and the feed additive has a positive promotion effect to green cultivation.