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Direct feeding type biological fermentation feed additive and preparation method and application thereof

A bio-fermentation feed and additive technology, applied in animal feed, animal feed, bacteria used in food preparation, etc., can solve problems such as improper use of bacteria, poor basic conditions, and complicated sources of bacteria

Inactive Publication Date: 2017-07-21
MINXIONG BIOTECH CO LTD LONGYAN FUJIAN +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0011] Problems in domestic fermented feed; (1) backward fermentation equipment and lack of professionals; because most fermented feed factories have little understanding of microorganisms and fermentation processes, in a sense, the conditions for producing fermented feed are not standardized, and most The manufacturer's fermentation equipment and professionals are very limited, and some basic conditions are particularly poor
It does not meet the hygienic requirements at all, and what is more serious is that there are no professionals among them to carry out microscopic inspection, so they are produced batch after batch, so that there are hidden dangers in product safety
(2) Poor management and hygienic conditions; anyone who is engaged in microbial fermentation knows that "disinfection and sterilization" work is one of the key links in the success or failure of fermentation
However, many fermented feed factories have imperfect management systems, chaotic logistics, serious problems in employee hygiene and production environment hygiene
(3) Fermentation of dangerous raw materials; many fermented feed manufacturers believe that mildewed raw materials can be detoxified as long as they are fermented, and can improve feed utilization and palatability of feed. This shows the basic knowledge of "fermentation" too little
(4) The problem of strain inactivation; due to the differences in the combination, screening, cultivation direction and methods of various microbial strains, the production level, the animal species used, fermentation processing, storage conditions and other factors, the production and application effects of microbial fermented soybean meal The difference is large, and the quality is difficult to guarantee
(5) The source of bacteria is complex; because these feed factories have poor understanding of fermentation, they are unwilling to spend money to purchase bacteria and production technology from formal units, and do not invite professionals to provide technical guidance, so the safety of bacteria cannot be guaranteed
No acute and subacute toxicity experiments, teratogenicity and disability experiments on animals have been done at all, and there is no product purpose efficacy verification. The unscientific content and ratio of probiotics lead to unstable efficacy of the product. Due to the complex intestinal environment of animals The number of beneficial bacteria contained in the microbial feed must reach a certain number and ratio to ensure its survival and function
Most manufacturers do not have enough understanding of micro-ecological fermentation, resulting in improper use of strains, and the quality of the products produced cannot be guaranteed stably

Method used

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  • Direct feeding type biological fermentation feed additive and preparation method and application thereof
  • Direct feeding type biological fermentation feed additive and preparation method and application thereof
  • Direct feeding type biological fermentation feed additive and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0071] Embodiment 1 Preparation of a direct-throwing type bio-fermentation feed additive

[0072] 1. Preparation of auxiliary materials: Commercially purchased soybean meal, wheat, and corn are routinely tested according to the feed standard, and then mixed according to the weight ratio of 6:1:3, then crushed, passed through a 80-mesh sieve, and passed through a drum dryer at high temperature (120-150°C) Dry and set aside.

[0073] 2. Preparation of lactic acid bacteria culture medium and bacteria powder:

[0074] (1) Medium preparation: Bifidobacterium solid medium was prepared as follows: peptone 5.0g, beef extract 5.0g, tryptone 10.0g, yeast powder 5.0g, glucose 8.0g, Tween 80 1.0g, K 2 HPO 4 2.0g, sodium acetate 5.0g, diammonium hydrogen citrate 2.0g, ZnSO 4 ·7H 2 O 0.25g, MgSO 4 ·7H 2 O 0.1g, distilled water to 1L, pH 6.2-6.4, autoclaved at 121°C for 20 minutes, the preparation of bifidobacterium liquid medium is the same as that of solid medium, but without adding...

Embodiment 2

[0092] Embodiment 2 Preparation of a direct-throwing type bio-fermentation feed additive

[0093] 1. Preparation of auxiliary materials: Commercially purchased soybean meal, wheat, and corn are routinely tested according to the feed standard and then mixed according to the weight ratio of 8:2:3, then crushed, passed through a 100-mesh sieve, and passed through a drum dryer at high temperature (120-150°C) Dry and set aside.

[0094] 2. Preparation of lactic acid bacteria culture medium and bacteria powder:

[0095] (1) Medium preparation: Bifidobacterium solid medium was prepared as follows: peptone 5.0g, beef extract 5.0g, tryptone 10.0g, yeast powder 5.0g, glucose 8.0g, Tween 80 1.0g, K 2 HPO 4 , agar 15.0g, 2.0g, sodium acetate 5.0g, diammonium hydrogen citrate 2.0g, ZnSO 4 ·7H 2 O 0.25g, MgSO 4 ·7H 2 O 0.1g, distilled water to 1L, pH 6.2-6.4, autoclaved at 121°C for 20 minutes, the preparation of bifidobacterium liquid medium is the same as that of solid medium, but w...

Embodiment 3

[0113] Embodiment 3 Preparation of a direct-throwing type bio-fermentation feed additive

[0114]1. Preparation of auxiliary materials: Commercially purchased soybean meal, wheat, and corn are routinely tested according to the feed standard and then mixed according to the weight ratio of 5:1:5, then crushed, passed through a 80-mesh sieve, and passed through a drum dryer at high temperature (120-150°C) Dry and set aside.

[0115] 2. Preparation of lactic acid bacteria culture medium and bacteria powder:

[0116] (1) Medium preparation: Bifidobacterium solid medium was prepared as follows: peptone 5.0g, beef extract 5.0g, tryptone 10.0g, yeast powder 5.0g, glucose 8.0g, Tween 80 1.0g, K 2 HPO 4 , agar 15.0g, 2.0g, sodium acetate 5.0g, diammonium hydrogen citrate 2.0g, ZnSO 4 ·7H 2 O 0.25g, MgSO 4 ·7H 2 O 0.1g, distilled water to 1L, pH 6.2-6.4, autoclaved at 121°C for 20 minutes, the preparation of bifidobacterium liquid medium is the same as that of solid medium, but wit...

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Abstract

The invention discloses a direct feeding type biological fermentation feed additive and a preparation method and an application thereof. The feed additive provided by the invention is composed of ingredients, fermentation probiotics, enzymic preparations and saline solution medium. The product can be directly applied to livestock breeding and aquaculture, and has the effects of improving immunity of animals, enhancing premonition, improving food consumption and a feed conversion ratio, treating and preventing enteric diseases of animals, adjusting balance of intestinal microecology of animals and resisting stress. According to the method provided by the invention, the production strains are screened in a combined manner, a purification method is completed, production time of the strains is subjected to strict quality control, and the problem that biological fermentation is instable is solved. The feed additive is directly used after fermentation, the condition that no damage is caused to all bioactivators generated after fermentation is ensured, and meanwhile the problems about transportation and bacterial contamination in storage are also avoided, so that the effect and security of the product are improved, the cultivation cost is greatly reduced, and the feed additive has a positive promotion effect to green cultivation.

Description

technical field [0001] The invention relates to a direct-throwing type biological fermentation feed additive and its preparation method and application, in particular to a method for improving animal immunity, enhancing disease resistance, increasing feed intake and feed conversion rate to treat and prevent animal intestinal diseases , A bio-fermented feed additive that regulates the micro-ecological balance and anti-stress ability of animals' intestines. The invention belongs to the technical field of biological fermentation feed additive research. Background technique [0002] As a new type of feed, biological fermented feed has attracted more and more attention from the feed industry in the 21st century. Therefore, biological feed has great development prospects in both theoretical research and practical application. [0003] Biological feed meets the needs of human beings for health food. In the 21st century, human health food will be safe, pollution-free, residue-free,...

Claims

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Application Information

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IPC IPC(8): A23K10/37A23K10/30A23K10/12A23K10/14A23K20/10A23K20/20A23K20/26A23K10/20A23K20/163A23K50/30
CPCA23V2400/137A23V2400/125A23V2400/113A23V2400/169A23V2400/51Y02P60/87
Inventor 陈敏张晶潘朝阳李建秋
Owner MINXIONG BIOTECH CO LTD LONGYAN FUJIAN
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