Tufted bamboo tissue culture rapid breeding rooting method

A technology for tissue culture, rapid propagation and bamboo, which is applied in horticultural methods, botanical equipment and methods, plant regeneration, etc., and can solve the problem that the rooting method and medium cannot achieve the effect, and it is impossible to cultivate seedlings in large quantities, and tissue culture seedlings can take root. Difficulties and other problems, to achieve the effect of important economic and social benefits

Inactive Publication Date: 2005-07-20
INST OF TROPICAL BIOSCI & BIOTECH CHINESE ACADEMY OF TROPICAL AGRI SCI +1
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Problems solved by technology

This bamboo species is only distributed sporadically in some areas of Yunnan Province, China, and its bamboo joints are very difficult to grow and take root. Traditional methods cannot be used to cultivate seedlings in large quantities.
It is the key to the development of dragon bamboo and its industry to study and use the method of tissue culture to obtain a large number of high-quality seedlings, but it is extremely difficult to take root of the tissue culture seedlings, and the conventional tissue culture rooting method and medium can not achieve the effect and cannot meet the demand

Method used

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Embodiment Construction

[0008] The present invention will be described in detail below in conjunction with examples.

[0009] The rooting method of the tissue culture and rapid propagation of clustered bamboo designed in the present invention includes the establishment process of sterile strains, the process of subculture multiplication culture, the process of inducing rooting, and the process of transplanting test-tube seedlings.

[0010] 1. The establishment process of sterile strains is to carefully remove the leaves from selected young bamboo shoots, rinse them with tap water after soaking in detergent solution, cut the branches into small sections with joints, and use 70% -80% ethanol for surface disinfection, then take out the segments and put them into mercuric chloride aqueous solution for disinfection treatment, during which the bamboo segments are constantly immersed in the mercuric chloride aqueous solution; take out the segments with sterile water Rinse, and then dry the water with steril...

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Abstract

The present invention discloses a rooting method for tissue culture and quick propagation of bushy bamboo. Said method includes the following steps: using cladium of bamboo as explant, on the bud-inducing culture medium culturing and constructing sterile line, on the enrichment culture medium making sterile seedling undego the bushy bud quick proparation process to form lots of sprouts, making plant division and strengthening seedlings, then successively inoculating them into first rooting culture medium and second rooting culture medium so as to culture and induce out complete root system.

Description

technical field [0001] The invention relates to a rooting method of clump bamboo, in particular to a rooting method for rapid propagation through tissue culture. Background technique [0002] Bamboo is an important forest resource. It has strong regeneration ability, rapid growth, short maturity period and high yield. It has the characteristics of "permanent" utilization once afforestation, and is known as "an undefeated industry". Its bamboo has the characteristics of good longitudinal strength, high elasticity, wear resistance, corrosion resistance, bending resistance, moth resistance, and flame retardancy. Compared with wood, it has the advantages of strong hardness, high stability, good glue degree, and beautiful appearance. . The annual output of bamboo in the world is about 16 million tons. In addition to the traditional use for building houses, making furniture and handicrafts, it is mainly used for making bamboo wood-based panels and high-grad...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 杨本鹏张树珍昝丽梅
Owner INST OF TROPICAL BIOSCI & BIOTECH CHINESE ACADEMY OF TROPICAL AGRI SCI
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