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Rapid propagation and seedling growing method for Shuhua camellia tissue culture

A camellia bunch and tissue culture technology, applied in the field of plant tissue culture, can solve the problems of difficulty in meeting new varieties, difficult rooting of camellia tissue culture, long production cycle, etc., to shorten the time to market, meet the requirements of fast-growing seedlings in factories, and simplify the production process. Effects of production steps

Active Publication Date: 2015-01-07
SHANGHAI BOTANICAL GARDEN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a method for the rapid propagation and seedling cultivation of camellia japonica through tissue culture, to overcome the difficulty in rooting of camellia tissue culture, and the low propagation coefficient and long production cycle in the traditional cutting propagation method, which is difficult to meet the short-term and large-scale listing of new varieties In order to meet the difficulties such as demand, the reproductive efficiency of Shuhua Camellia can be greatly improved, the cycle to market can be shortened, and it can produce seedlings with no pests and diseases and strong consistency. It has strong pertinence, good adaptability, and strong operability, and realizes the industrialization of Shuhua Camellia. Rapid seedling production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] (1) Selection and disinfection of explants:

[0027] In the morning when the weather is fine, a new variety of Shuhua camellia "Xiaofenyu" (variety right number: 20120001) is selected, and the terminal buds that are free from diseases and insect pests, plump and just germinated but not yet unfolded are used as explants.

[0028] (2) Pretreatment and disinfection of explants:

[0029] Gently scrub the explants with detergent for 2-3 times, wrap them in gauze, and rinse them under running water for 20-30 minutes. Soak in 1000mg / L cephradine solution for 1 hour, and rinse with clean water. After washing, disinfect with 75% alcohol for 90 seconds, then disinfect with 0.1% mercury liter for 10 minutes, and finally wash with sterile water for 3 to 5 times, and set aside.

[0030] (3) Induction culture:

[0031] Under sterile conditions, peel off the scales and 2-3 layers of leaves wrapped in the outer layer of the terminal bud after disinfection, and then inoculate on the ...

Embodiment 2

[0035] (1) Selection of explants:

[0036] On a sunny morning, a new Shuhua camellia variety "Meiyu" (variety right number: 20120054) free from diseases and insect pests, plump and newly germinated but not yet unfolded terminal buds was selected as explants.

[0037] (2) Pretreatment and disinfection of explants:

[0038] Gently scrub the explants with detergent for 2-3 times, wrap them in gauze, and rinse them under running water for 20-30 minutes. Soak in 2000mg / L cephradine solution for 1 hour, and rinse with clean water. After washing, disinfect with 75% alcohol for 90 seconds, then disinfect with 0.1% mercury liter for 12 minutes, and finally wash with sterile water for 3 to 5 times, and set aside.

[0039] (2) Induction culture: Under sterile conditions, peel off the scales and 2-3 layers of leaves wrapped by the outer layer of the explant, and then inoculate it on the induction medium. The culture conditions are: the temperature is 24±1°C, the light intensity is 1500...

Embodiment 3

[0043] The rooting solution is 0.5 mg / L naphthaleneacetic acid (NAA), and the others are the same as in Example 1.

[0044] The rooting rate of aseptic seedlings cultivated in this implementation is 91%, and the survival rate of transplanting is 90%. Requirements for industrialized seedling cultivation.

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Abstract

The invention discloses a rapid propagation and seedling growing method for Shuhua camellia tissue culture. The rapid propagation and seedling growing method comprises the following steps of: selecting an explant of Shuhua camellia, pretreating and sterilizing; carrying out induced differential culture on an induced culture medium, and carrying out enrichment culture on a multiplication culture medium; then transplanting aseptic seedlings obtained through multiplication directly into a matrix for rooting. The rapid propagation and seedling growing method provided by the invention discloses that the camellia can be subjected to rooting culture without an aseptic condition firstly, and the aseptic seedlings cultured by the multiplication are directly treated by rooting liquid, and then are cut into the gardening matrix for the rooting; the rooting rate is above 90%; the transplanting survival rate is above 86%; the problem of camellia tissue culture seedlings are difficult in the rooting is solved; the production steps are simplified; the cultivation cycle is shortened to be within 5 months; the explant multiplication rate is above 300%; the production efficiency is improved; the market cycle is shortened greatly; the requirement of factory-like rapid propagation and seedling growing can be met.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and in particular relates to a method for rapid propagation and seedling cultivation of camellia japonica through tissue culture. Background technique [0002] Shuhua camellia is a new variety of camellia hybridized with Lian Rui tea (Sect. . Shuhua camellia is a new variety of camellia, which is different from the relatively single application form of traditional camellia varieties such as solitary planting and group planting. Shuhua camellia can be used as flower hedges, hedgerows, bonsai and landscape trees, with high ornamental value and economic value. At present, the protection of new plant varieties in China is relatively low, and the promotion of new varieties is prone to the risk of being plagiarized by others. Therefore, it is one of the current strategies for the promotion of new varieties to go public on a large scale to generate economies of scale and price advantages...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 刘炤奉树成张亚利蔡新凤费建国李健
Owner SHANGHAI BOTANICAL GARDEN
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