Plant drought-resistant relevant protein PvSnRK 2.3 and encoding gene and application thereof

A technology related to proteins and plants, applied in the field of genetic engineering, can solve problems affecting wheat yield and quality, and restricting wheat food security, etc.

Inactive Publication Date: 2012-11-07
BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, every year due to adversity stress conditions such as drought and salinity, the output of my country's wheat is reduced by about 80 billion kilograms, which seriously affects the yield and quality of wheat and restricts my country's wheat food security.

Method used

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  • Plant drought-resistant relevant protein PvSnRK 2.3 and encoding gene and application thereof
  • Plant drought-resistant relevant protein PvSnRK 2.3 and encoding gene and application thereof
  • Plant drought-resistant relevant protein PvSnRK 2.3 and encoding gene and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0070] Example 1: cDNA Cloning of PvSnRK2.3 Gene Related to Drought Resistance in Switchgrass.

[0071] The 30-day-old switchgrass seedlings were subjected to drought treatment for 5 hours, and the total RNA of switchgrass was extracted with Trizol. 5'RACE kit (5'RACE System for Rapid Amplification of cDNA Ends Kit) (GIBCOBRL, CAT.NO.18374-058) and 3'RACE kit (3'RACE System for Rapid Amplification of cDNA Ends Kit) ( GIBCOBRL, CAT.NO.18373-019) obtained the full-length sequence of PvSnRK2.3 gene 1002bp.

[0072] The total RNA of switchgrass seedlings was extracted with Trizol, and cDNA was obtained by reverse transcription with superscript II (invitrogen) reverse transcriptase. Primers P1 and P2 were designed according to the sequence of the coding region of PvSnRK2.3 gene. Using the cDNA obtained by reverse transcription as a template, PCR amplification was performed with primers P1 and P2. The sequences of primers P1 and P2 are as follows:

[0073] P1: 5'-ATCGTCCAGGAGGCC...

Embodiment 2

[0077] Example 2: Enhancement of plant drought tolerance with PvSnRK2.3 gene

[0078] 1. Construction of recombinant expression vector

[0079] 1) Construction of 35S-PvSnRK2.3 recombinant expression vector

[0080] Using the cDNA obtained by reverse transcription of switchgrass total RNA as a template, PCR amplification was performed with specific primers containing SmaI and SpeI linker sequences; then SmaI and SpeI double-enzyme-digested PCR products were recovered, and the enzyme-digested products were inserted forward into the vector pBI121 Between the SmaI and SpeI restriction sites behind the CaMV35S promoter, the recombinant vector p35S-PvSnRK2.3 was obtained.

[0081] The primer sequences are as follows:

[0082] PvSnRK2.3[SmaI]5'-TC CCCCGG GATGGAGGAGAGGTAC-3'

[0083] PvSnRK2.3[SpeI]5'-GG ACTAGT TCAGTAGGCGTCATCGGCG-3'

[0084] 2. Acquisition and functional identification of transgenic Arabidopsis

[0085] 1) Obtaining transgenic Arabidopsis

[0086] The reco...

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Abstract

The invention relates to the field of genetic engineering, in particular to plant drought-resistant relevant protein PvSnRK 2.3 and an encoding gene and application thereof. The drought-resistant relevant protein PvSnRK 2.3 is from panicum virgatum, and an amino acid sequence of the drought-resistant relevant protein PvSnRK 2.3 is shown as SEQ ID NO. 1. The drought-resistant relevant protein and the encoding gene thereof have the important theoretical and practical significance for improving and enhancing the stress resistance of arabidopsis, improving yield, accelerating the anti-stress molecular breeding progress and saving water resources effectively.

Description

technical field [0001] The invention relates to the field of genetic engineering, in particular, the invention relates to a plant drought resistance-related protein PvSnRK2.3 and its coding gene and application. Background technique [0002] As one of the important food crops in our country, wheat occupies a very important position in the national economy. However, adversity stress conditions such as drought and salinity reduce the output of my country's wheat by about 80 billion kilograms every year, seriously affecting the yield and quality of wheat, and restricting my country's wheat food security. With the development of modern molecular biology, genetic engineering technology is used to study the relationship between plants and abiotic stress at the molecular level, reveal the molecular mechanism of plant stress signal transduction and gene expression regulation, and provide new ways for cultivating new stress-resistant crops. quality provides a theoretical basis. [...

Claims

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Application Information

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IPC IPC(8): C12N9/12C12N15/54C12N15/63C12N5/10C12N1/21C12N15/82C12N15/84A01H5/00
Inventor 高世庆赵昌平唐益苗徐蓓
Owner BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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