Method for constructing collard cytoplasmic male sterility near-isogenic lines

A near-isogenic line and kale technology, which is applied in the field of flower breeding, can solve the problems such as the near-isogenic line of cytoplasmic male sterility in kale that has not yet been seen.
CN101878734AInactive Publication Date: 2010-11-10SHENYANG AGRI UNIV
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
SHENYANG AGRI UNIV
Publication Date
2010-11-10
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a method for constructing collard cytoplasmic male sterility near-isogenic lines, which is characterized by comprising the following steps of: A, selecting the variety of collard, performing selfing to the ninth generation, and using the ninth generation as a receptor; B, selecting a cytoplasmic male sterile Chinese cabbage selfed line as an CMS donor; C, using the donor as a female parent, using the receptor as a male parent, performing interspecific hybridization to obtain interspecific hybrid, and successfully transforming the CMS genes to collard; D, performing authenticity identification on the interspecific hybrid; E, using the interspecific hybrid in the interspecific hybridization as a female parent, using a receptor collard selfed line as a recurrent parent, and performing back crossing to the sixth generation to breed a collard cytoplasmic male sterility line which forms a pair of near-isogenic lines with the recurrent male parent; and F, analyzing the near-isogenic property between the back-crossed sixth generation and the recurrent parent. The invention provides a method for constructing collard cytoplasmic male sterility near-isogenic lines, obtains the collard cytoplasmic male sterility near-isogenic lines, and provides a parent material for producing advantageous hybrid of the collard.
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Description

technical field

[0001] The invention belongs to the technical field of flower breeding, and in particular relates to a method for constructing a cytoplasmic male sterile near-isogenic line of kale. Background technique

[0002] Kale (Brassica oleracea var. acephala L) is a Brassica flower of the family Brassicaceae, native to the Mediterranean coast. The central leaves of kale in the rosette stage are gorgeous in color, wrinkled in shape, strong in cold resistance, and have important economic and ornamental value.

[0003] Kale is a cross pollinated crop with obvious heterosis. The male sterile line of kale can avoid artificial castration in the hybrid seed production of kale, which not only saves time and effort, but also ensures the purity of the hybrid, which is of great significance for the utilization of heterosis in kale. The use of male sterile lines for seed production is one of the main ways to utilize heterosis in vegetable oil crops of the same genus.

[0004] ...

Claims

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