Method for constructing collard cytoplasmic male sterility near-isogenic lines

A near-isogenic line and kale technology, which is applied in the field of flower breeding, can solve the problems such as the near-isogenic line of cytoplasmic male sterility in kale that has not yet been seen.

Inactive Publication Date: 2010-11-10
SHENYANG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

There is no method for constructing a near-iso

Method used

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  • Method for constructing collard cytoplasmic male sterility near-isogenic lines
  • Method for constructing collard cytoplasmic male sterility near-isogenic lines
  • Method for constructing collard cytoplasmic male sterility near-isogenic lines

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1: Male sterile near-isogenic lines k13 and CMSk13 of kale were successfully constructed.

[0024] The specific method is as follows:

[0025] A. Recipient Parental Breeding: See figure 1 , select kale commercial variety 0113, carry out self-purification, until the 9th generation of self-cross (F 9 ), bred the kale inbred line k13, and the inbred line k13 was highly uniform among the lines. The typical characters of the inbred line k13 are shown in Table 1. The inbred line k13 was used as the recipient parent.

[0026] Table 1 Recipient parent k13, donor parent A02 and their interspecific hybrid F 1(A02-k13) Comparison of 11 typical traits of

[0027]

[0028] B. Preparation of the donor parent: the cytoplasmic male sterile head cabbage inbred line A02 was selected as the CMS donor parent. The typical traits of inbred line A02 are shown in Table 1.

[0029] C. Interspecific hybridization: see figure 2 , with the donor parent A02 as the female parent ...

Embodiment 2

[0048] Example 2: Male sterile near-isogenic lines k10 and CMSk10 of kale were successfully constructed.

[0049] The specific method is as follows:

[0050] A. Recipient Parental Breeding: See image 3 , the commercial variety 0113 of kale was selected, selfed and purified, and the kale inbred line k10 was bred until the ninth generation of selfing, and the lines of the inbred line k10 were highly uniform. The typical morphological characteristics of the inbred line k10 are shown in Table 4. The inbred line k10 was used as the recipient parent.

[0051] Table 4 Recipient parent k10, donor parent A02 and their interspecific hybrid F 1(A02-k10) Comparison of 11 typical traits of

[0052]

[0053] B. Preparation of the donor parent: the cytoplasmic male sterile head cabbage inbred line A02 was selected as the CMS donor parent. The typical morphological characteristics of inbred line A02 are shown in Table 4.

[0054] C. Interspecific hybridization: see Figure 4 , with...

Embodiment 3

[0065] Example 3: Male sterile near-isogenic lines k04 and CMSk04 of kale were successfully constructed.

[0066] The specific method is as follows:

[0067] A. Recipient Parental Breeding: See Figure 5 , the commercial variety 0104 of kale was selected, selfed and purified, and the kale inbred line k04 was bred to the ninth generation of selfing, and the lines of the inbred line k04 were highly uniform. The typical morphological characteristics of the inbred line k04 are shown in Table 7. The inbred line k04 was used as the recipient parent.

[0068] B. Preparation of the donor parent: the cytoplasmic male sterile head cabbage inbred line A02 was selected as the CMS donor parent. The typical morphological characteristics of inbred line A02 are shown in Table 7.

[0069] C. Interspecific hybridization: see Figure 6 , with the donor parent A02 as the female parent and the recipient parent k04 as the male parent, interspecific crosses were carried out. The cross pollinat...

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Abstract

The invention discloses a method for constructing collard cytoplasmic male sterility near-isogenic lines, which is characterized by comprising the following steps of: A, selecting the variety of collard, performing selfing to the ninth generation, and using the ninth generation as a receptor; B, selecting a cytoplasmic male sterile Chinese cabbage selfed line as an CMS donor; C, using the donor as a female parent, using the receptor as a male parent, performing interspecific hybridization to obtain interspecific hybrid, and successfully transforming the CMS genes to collard; D, performing authenticity identification on the interspecific hybrid; E, using the interspecific hybrid in the interspecific hybridization as a female parent, using a receptor collard selfed line as a recurrent parent, and performing back crossing to the sixth generation to breed a collard cytoplasmic male sterility line which forms a pair of near-isogenic lines with the recurrent male parent; and F, analyzing the near-isogenic property between the back-crossed sixth generation and the recurrent parent. The invention provides a method for constructing collard cytoplasmic male sterility near-isogenic lines, obtains the collard cytoplasmic male sterility near-isogenic lines, and provides a parent material for producing advantageous hybrid of the collard.

Description

technical field [0001] The invention belongs to the technical field of flower breeding, and in particular relates to a method for constructing a cytoplasmic male sterile near-isogenic line of kale. Background technique [0002] Kale (Brassica oleracea var. acephala L) is a Brassica flower of the family Brassicaceae, native to the Mediterranean coast. The central leaves of kale in the rosette stage are gorgeous in color, wrinkled in shape, strong in cold resistance, and have important economic and ornamental value. [0003] Kale is a cross pollinated crop with obvious heterosis. The male sterile line of kale can avoid artificial castration in the hybrid seed production of kale, which not only saves time and effort, but also ensures the purity of the hybrid, which is of great significance for the utilization of heterosis in kale. The use of male sterile lines for seed production is one of the main ways to utilize heterosis in vegetable oil crops of the same genus. [0004] ...

Claims

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Application Information

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IPC IPC(8): A01H1/02
Inventor 祝朋芳魏毓棠陈长青周家野张月赵颖王兴张健
Owner SHENYANG AGRI UNIV
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