A sterilization composition containing copper quinolate and zhongshengmycin is disclosed. The effective compositions copper quinolate and zhongshengmycin are subjected to two-composition combination, and the rest is auxiliary compositions. The mass ratio of copper quinolate to zhongshengmycin in the pesticide composition is 1-50:50-1, the mass proportion of the effective compositions copper quinolate and zhongshengmycin in the preparation is 1%-80%, and the rest is acceptable auxiliary compositions allowed to be used in pesticide. The dosage forms of the pesticide composition are missible oil, a suspending agent, a wettable powder, a water dispersable granule, an emulsion in water, a micro emulsion, a granule and a micro capsule. The sterilization composition is mainly applied to control fruit tree rot disease, Alternaria Leaf Spot, rice blast, soybean root rot, cucurbits die-back, cotton verticillium wilt, jujube tree rust, grape white rot, panax notoginseng black spot, exobasidium vexans Ma-ssee, forest tree rot disease, canker, gummosis, leaf cast, tree seedling stage damping-off, erwinia carotovora, Pseudomonas syringae pv.lachrymans, xanthomonas oryzae pv.Oryzae, botryospuaeria berengeriana de Not .t .sp .Piricola, fusarium graminearum and the like.