Biocontrol bacterial strain SM16 for preventing pear die-back

A biocontrol strain, SM16 technology, applied in the direction of bacteria, biocides, microorganisms, etc., can solve the problems of increased use times and drug concentration, damage to the micro-ecological environment, etc., and achieve the effect of saving costs

Inactive Publication Date: 2010-10-20
NANJING AGRICULTURAL UNIVERSITY
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  • Abstract
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Problems solved by technology

However, the long-term single use of agricultural streptomycin has led to an increase in the frequency of use and the concentration of the drug, and has also increased the risk of pathogenic bacteria developing resistance to agricultural streptomycin
At the same time, due to the broad spectrum of streptomycin, extensive use not only destroys the micro-ecological environment, but also leads to non-target resistance

Method used

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  • Biocontrol bacterial strain SM16 for preventing pear die-back
  • Biocontrol bacterial strain SM16 for preventing pear die-back
  • Biocontrol bacterial strain SM16 for preventing pear die-back

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Embodiment Construction

[0023] A biocontrol strain SM16 for the control of pear dieback disease, isolated from rice fields in Zhenjiang City, Jiangsu Province, is Bacillus subtilis, the cells are rod-shaped, arranged in chains, the ports are round, and the spores are oval or round , monospore, facultative aerobic, able to utilize rhamnose, glucose, arabinose, lactose, mannitol, xylose, maltose, D-fructose, D-mannose, sorbose, optimum growth temperature 35-37℃ , the colony is milky white, the edges are neat, the surface has wrinkles and bulges, the surface of the colony is dry, G+, the colony is opaque, the M.R test, V.P test, and catalase test are all positive, and malonate can be used.

[0024] The method that biocontrol strain SM16 is used to prepare biocontrol agent is:

[0025] 1) Inoculate the strain into LB culture medium and culture at 30°C with shaking at 180rpm. After 12 to 16 hours, take samples in the ultra-clean workbench and measure the OD value at 600nm. During the measurement, use the ...

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Abstract

The invention relates to a biocontrol bacterial strain SM16 for preventing pear die-back, belonging to the technical field of plant protection. The strain is bacillus subtilis prepared by the following steps of: vaccinating the strain in culture liquid; fermenting at 30 DEG C and 250 rpm for 20-24 h; then, centrifuging at 6000 rpm for 10 min; and preparing a bacterial agent from wet bacteria and a cell preservation solution in a ratio of 1:40 (g/ml), wherein the total concentration of live viable bacteria in a finished product is 1*109-1*1012 cfu/ml. When the biocontrol bacterial strain SM16 for preventing pear die-back is used in a fruit garden and 25 percent and 75 percent of pear flowers are blossomed, the bacterial agent is diluted into 1*108 cfu/ml by adding water and sprayed on the flower surfaces and has the pear die-back prevention effect over 57-68 percent.

Description

1. Technical field [0001] The invention relates to a Bacillus subtilis biocontrol strain SM16 for preventing and treating pear dieback disease, which belongs to the technical field of fruit tree disease prevention and control, and is specially used for pollution-free prevention and control of pear dieback disease. 2. Background technology [0002] Pear dieback disease is caused by bacteria of the genus Erwinia, which has a great impact on the pear industry. The fungus infects pear trees at the beginning of the flowering stage, and the infected flower organs produce water-soaked lesions, which soon wither and turn black and die. Under humid conditions, bacterial pus oozes from infected flower organs, fruit stalks, petioles and other tissues. Infected pear branches wilt and die, and the dead leaves do not fall off. Branches wither and develop quickly. When the conditions are suitable, they can expand by more than 15-30cm within a few days, resulting in the death of whole bra...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/02A01P1/00C12R1/125
Inventor 郭坚华李师默陈云
Owner NANJING AGRICULTURAL UNIVERSITY
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