Agrobacterium rhizogenes transformation and expression of rol genes in kalanchoe

a technology of rol genes and agrobacterium rhizogenes, which is applied in the field of kalanchoe interspecific hybrid plants to achieve the effect of reducing the height of kalancho interspecific hybrid plants

Active Publication Date: 2016-02-04
UNIVERSITY OF COPENHAGEN +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Kalanchoë displays an elongated growth habit in nature, which is considered undesirable for the potted plant industry that favors more compact plant architecture for space and transportation purposes.

Method used

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  • Agrobacterium rhizogenes transformation and expression of rol genes in kalanchoe
  • Agrobacterium rhizogenes transformation and expression of rol genes in kalanchoe
  • Agrobacterium rhizogenes transformation and expression of rol genes in kalanchoe

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Experimental program
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Effect test

example 1

Transformation Materials and Methodology

Plant Material

[0075]In vivo plants of Kalanchoë grandiflora and the F1-hybrid 2009-0347 (referred to as 0347) and established in vitro culture plants of K. blossfeldiana ‘Molly’, and K. grandiflora, and the F1-hybrid 2006-0199 (referred to as 0199), (Knud Jepsen A / S, Hinnerup, Denmark and KU-LIFE, Crop Sciences, Tåstrup). In vivo plants were cultivated in a greenhouse with temperatures of 20° C. at day and night, 16 hour day length and a light intensity of 260 μmol photons m−2 s−1. In vitro plants were cultivated in growth chamber with temperatures of 25° C. at day and 22° C. at night, 13 hour day length and a light intensity of 75 μmol photons m−2 s−1. The two F1-hybrids are closely related since 0199 is the paternal part of the crossing to produce 0347.

[0076]36, 104, 166, 158 and 128 leaf explants were used for transformation of K. blossfeldiana ‘Molly’, 2006-0199, 2009-0347, K. grandiflora and K. grandiflora, respectively. 25 leaf explants ...

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Abstract

The present disclosure embraces Kalanchoë interspecific hybrid plants, and considers rol transformation in Kalanchoë species and hybrids. Disclosed herein are methodology and the like for producing rol-transformed Kalanchoë interspecific hybrid plants, as well as resultant rol-transformed Kalanchoë interspecific hybrid plants with novel phenotypes.

Description

FIELD[0001]The present disclosure embraces Kalanchoë interspecific hybrid plants, and considers rol transformation in Kalanchoë species and hybrids.INTRODUCTION[0002]Kalanchoë blossfeldiana, and its cultivars, is a horticulturally important plant due to its popularity as both an indoor and outdoor plant. The genus of Kalanchoë belongs to the sedum family (Crassulaceae), and there are more than 100 different species of Kalanchoë, most of which are found in Madagascar and South Africa, and a few in Asia and South America. Kalanchoë are succulent plants, characterized by turgid leaves that enable the plants to survive drought conditions. Consequently, Kalanchoë are useful ornamental plants because they can survive in less than optimal growing conditions.[0003]Kalanchoë displays an elongated growth habit in nature, which is considered undesirable for the potted plant industry that favors more compact plant architecture for space and transportation purposes. Thus, the industry treats Kal...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): C12N15/82
CPCC12N15/8205C12N15/8261A01H3/00Y02A40/146A01H6/32
Inventor CHRISTENSEN, ELLEN MARGRETHE SKOVSGAARDMULLER, RENATE PETRA BRIGITTELUTKEN, HENRIK VLKHEGELUND, JOSEFINE NYMARKJENSEN, LINECHRISTENSEN, BRIANJENSEN, ELSE BOLLERUPNIELSEN, KAI LONNE
Owner UNIVERSITY OF COPENHAGEN
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