Method for quickly obtaining interspecific hybrids of incompatible wide species and cultivated species of peanuts

A technology of wild species and cultivated species, applied in the field of plant distant hybridization, can solve the problems of lengthy and cumbersome tissue culture steps, and achieve efficient results

Inactive Publication Date: 2012-07-18
SHANDONG PEANUT RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the above methods all require lengthy and cumbersome tissue culture steps, and there is

Method used

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  • Method for quickly obtaining interspecific hybrids of incompatible wide species and cultivated species of peanuts
  • Method for quickly obtaining interspecific hybrids of incompatible wide species and cultivated species of peanuts
  • Method for quickly obtaining interspecific hybrids of incompatible wide species and cultivated species of peanuts

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Method of the present invention comprises the steps:

[0023] (1) Prepare a hormone mixture containing 2 mg / L indole acetic acid, 2 mg / L gibberellin, 2 mg / L 6-benzylaminopurine, and sterile deionized water as the rest.

[0024] (2) Collect the pollen of the incompatible wild species of peanut, and transfer to the female parent of the cultivated species of peanut. The pollination process is the same as the routine.

[0025] (3) Dip the hormone mixture solution with absorbent cotton, and apply it to the base of the female parent flower of the pollinated cultivar.

[0026] (4) After the seeds were harvested, thin slices of peanut cotyledon tissue were taken, and the DNA of the sample to be tested was quickly extracted as a PCR template by using the "Simple and Rapid DNA Extraction Method from Peanut Healthy and Diseased Tissues" (Patent No.: 200910255786.0).

[0027] (5) Identify the extracted template by using the "rapid typing method for peanut Δ12-fatty acid dehydrogen...

Embodiment 2

[0030] Method of the present invention comprises the steps:

[0031] (1) Prepare a hormone mixture containing 4 mg / L indole acetic acid, 2 mg / L gibberellin, and sterile deionized water as the rest.

[0032] (2) Collect the pollen of the incompatible wild species of peanut, and transfer to the female parent of the cultivated species of peanut. The pollination process is the same as the routine.

[0033] (3) Dip the hormone mixture solution with absorbent cotton, and apply it to the base of the female parent flower of the pollinated cultivar.

[0034] (4) After harvesting the seeds, thin slices of peanut cotyledon tissue were taken, and the DNA of the sample to be tested was quickly extracted as a PCR template by using the "Simple and Rapid DNA Extraction Method for Peanut Healthy and Diseased Tissues" (Patent No.: 200910255786.0).

[0035] (5) Identify the extracted template by using the "rapid typing method for peanut Δ12-fatty acid dehydrogenase gene" (patent application num...

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Abstract

The invention relates to a plant distant hybridization technology, in particular to a method for quickly obtaining a large amount of interspecific hybrids of an incompatible wide species and a cultivated species of peanuts through medicine induction after pollination. The method for quickly obtaining the interspecific hybrids of the incompatibility wide species and the cultivated species of the peanuts comprises the following steps of: (1) preparing a hormone mixed solution; (2) collecting incompatible peanut wide species pollen and pollinating a peanut cultivated species female parent; (3) dipping the hormone mixed solution and applying on a base of each pollinated cultivated species female parent flower; and (4) carrying out molecular identification on the harvested seeds. According to the invention, the method can be operated in the field and a large amount of interspecific hybrids can be effectively obtained, a long-winded complicated tissue cultivation step is omitted, and the incompatible hybrids of the peanuts can be simply and easily obtained.

Description

technical field [0001] The invention relates to plant distant hybridization technology, in particular to a method for quickly and massively obtaining hybrids between peanut incompatible wild species and cultivated species through drug induction after pollination. Background technique [0002] Peanut is one of the five most important oil crops in the world. Inter-variety hybridization is still the main method of peanut breeding so far, but due to the narrow genetic basis of peanut cultivars, it is becoming more and more difficult to breed breakthrough varieties through this method. More and more peanut breeding workers realize that the genetic diversity of peanut wild species, especially incompatible wild species outside the peanut block group, is significantly higher than that of cultivated species, rich in resistance, high-quality and high-yield genetic factors, and should be used in Used in peanut breeding. [0003] The wild species of peanut blocks are easily crossed wi...

Claims

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Application Information

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IPC IPC(8): A01H1/02
Inventor 王传堂于洪涛王秀贞唐月异吴琪陈殿绪崔凤高高华援凤桐唐荣华胡东青禹山林
Owner SHANDONG PEANUT RES INST
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