51 results about "Heterodea muelleri" patented technology

The invention discloses a novel germplasm creating method by distant hybridization of Brassica campestris ssp. chinensis Makino and Raphanus sativus. The method uses autotetraploid Brassica campestris ssp. chinensis Makino as a female parent and autotetraploid Raphanus sativus as a male parent to conduct artificial sexual crossbreeding; and repeated pollination and in vitro embryo rescue techniques are employed to obtain distant hybridization novel germplasm of autotetraploid Brassica campestris ssp. chinensis Makino and autotetraploid Raphanus sativus. The invention for the first time adopts sexual crossbreeding of the autotetraploid Brassica campestris ssp. Chinensis Makino and autotetraploid Raphanus sativus and in vitro embryo rescue technique to obtain new germplasm of distant hybridization. Compared with traditional diploid hybridization, which obtains zygotic embryo for ploidy reduplication, the method does not require chromosome doubling operation, and can significantly shorten the breeding time; and the hybrid F1 can reach stable inheritance. The novel germplasm created by the invention creation has the genetic materials of Brassica campestris ssp. chinensis Makino and Raphanus sativus, and a wider genetic diversity.

The invention discloses a cultivation method for new okra varieties. The cultivation method includes seeding and planting management and sexual hybridization of okra parents, pollen activity of the okra parents is tested, the okra parents are assisted in sexual hybridization by means of manual castration and pollination when the pollen activity of the okra parents reaches more than 30%, seeds are collected, hybrid progenies are screened and purified, expressed characters of the parents and the hybrid progenies are compared in the same planting environment, the hybrid progenies with excellent characters are screened out and inbred, and the excellent characters are purified and stabilized. The okra varieties 'Wu Fu' and 'pentagon' are used as female parents, red okra is used as a male parent, sexual hybridization is performed by means of manual pollination, and the obtained new okra varieties have the advantages of nutrient-rich pods, long collecting period, high yield per mu and the like as compared with the female parents 'Wu Fu' and 'pentagon', have the advantages of excellent pod quality, short plant form, lodging resistance and the like as compared with the male parent red okra, and are novel healthcare new vegetable varieties with excellent quality.

The invention relates to a method for cultivating Lilium oriental hybrid embryos, which is effective in breeding effect and short in breeding period. The method includes: pollinating a Lilium oriental hybrid line; sectioning and peeling small embryos, ovules or ovaries; and performing isolated culture. By cross pollination of the ovaries and young embryos and isolated culture, the embryos can be taken out for culturing before abortion to avoid premature senility of distant hybrid embryos, and accordingly Lilium post-fertilization barrier can be overcome effectively, a great amount of distant hybrid embryos are allowed to continue growing into normal seeds, a breeding procedure is drawn close as soon as possible, and the breeding cycle is shortened. In addition, a great amount of distant hybrids are obtained, Lilium interspecific distant hybridization is achieved, and a batch of novel germplasm is created. The embryo rescue technology and the technology of within-bottle Lilium bulbing are combined, so that culture cycle of Lilium hybrids is shortened effectively, and hybrid survival rate is increased.

Disclosed is a cultivation method for reserving seeds of globe artichoke. The cultivation method includes greenhouse selection, seedling culturing, transplantation, field management, seed collection and the like. The field management specifically includes managements of transplanting time, winter management technology of seedling earlier stage, spring management technology of seedling later period, bud stage, flowering period and grain filling stage. The cultivation method for reserving seeds of globe artichoke has the advantages that the costs are reduced, each seed from foreign countries is 0.5 yuan, and each seed obtained by the scheme is 0.1 yuan; the supply is timely, the seeds from the foreign countries are ripe basically in August but sowing is required in September in China so that season connection is not timely, and the seeds obtained by the scheme can be completely ripe in July without delay of sowing of the next season; the seeds obtained by the scheme are produced in local area, advantages exist in terms of area adaptability, and the number of hybrid seeds and hybrid plants is small.

The invention belongs to the field of transgenic technologies, and relates to a method for improving the fertility of indica-japonica rice hybrids by virtue of LRK1 gene transformation. According to the method, rice LRK1 genes are transformed into indica calluses by virtue of a gene gun microprojectile bombardment method, and rice plants for expressing the LRK1 genes are obtained through recovery culture. Experimental results show that the LRK1 genes can be used for successfully transforming rice and hybridizing rice with japonica rice varieties such as Nipponbare and Akihikari respectively, and the F1-generation seed setting rates are greatly higher than the control so as to achieve the seed setting rate level of regular varieties. Rice is provided with wide compatibility and can overcome the infertility characteristic of indica-japonica hybrids after being transformed by the rice LRK1 genes provided by the invention and the homologous genes thereof. The invention provides a low-cost and high-benefit novel method for utilization for the advantages of indica-japonica rice intersubspecific hybrids.

The invention discloses a method for cultivating apple hybrid seedlings. The method includes the following steps: picking up ripe apple hybrid fruits, and storing the ripe apple hybrid fruits in a refrigeration house at the temperature of 1-4 DEG C; acquiring hybrid seeds from the apple hybrid fruits stored in the refrigeration house, and using clean water to clean the hybrid seeds; preparing a bed with the width of about 1 meter in a greenhouse, putting a medium in nutriment pots, straightening the medium in the bed, watering the medium thoroughly, and allowing the medium to stand for 2-3 days; sowing the cleaned hybrid seeds in the nutriment pots, covering the hybrid seeds with wet nutriment soil with the thickness of 1 cm, and controlling the temperature of the greenhouse at 25-30 DEG C; changing the temperature of the greenhouse to 20-25 DEG C when the height of hybrid seedlings is 5-10 cm, keeping the relative humidity at 50-70%, performing ventilation at suitable time, transplanting the hybrid seedlings to a nursery garden after the hybrid seedlings are cultivated for 15-20 days, watering the hybrid seedlings once after transplanting, and performing management after planting. The method can avoid sand storage and germination accelerating in an apple hybrid seedling cultivation process, is easy to operate, and can improve the rate of emergence by about 25%.

The invention discloses a cultivation method for high-oleic-acid peanuts with spotted seeds and peels. The cultivation method for high-oleic-acid peanuts with spotted seeds and peels comprises the following steps: separately selecting a pink-peel high-oleic-acid peanut and a spotted common peanut as a female parent and a male parent, carrying out hybridization to obtain F1, erasing false hybrid from the generation F1 and then carrying out selfing to obtain F2, screening a single strain with spotted seeds and peels from F2 by using a KASP label, screening F3 again to obtain a stable good-performance single strain with spotted seeds and peels, carrying out variety test on F4 line, and screening a candidate variety to finally obtain a F5 formation variety. The appearance of the cultivated high-oleic-acid peanuts with spotted seeds and peels is different from that of common pink-peel peanuts, the cultivated high-oleic-acid peanuts with spotted seeds and peels are conveniently distinguishedfrom common peanuts, and thus, the high-oleic-acid peanuts can be utilized well.

The invention provides a breeding method for selfing prior to hybridizing of whiteleg shrimp groups, which is characterized by including: selecting whiteleg shrimp populations with excellent seed sources, clear genetic background and close relation between individuals in the same population; selecting the individuals from the same population, and performing close inbreeding to produce self-bred offspring; selecting the self-bred offspring generated by close inbreeding based on merit, and establishing fundamental breeding populations subjected to inbreeding purification; selecting the fundamental breeding populations with character complementation for inter-population hybridization so as to obtain offspring seeds with heterosis. The method has the advantages that the fundamental breeding populations are established by selfing passage and selection, young shrimps produced by inter-population combined hybrid breeding are higher in resistance to low temperature and low saline, and the production speed in the cultivation period is faster.

The invention discloses an identification method of interspecific hybrids of Brassica campestris and Brassica napus, falling into the technical field of plant cytology. The method includes collecting the 4th-7th leaves of a plant in the seedling stage, tearing to collect hypoderm tissues of lower parts of the leaves, staining with iodine-potassium iodide solution, observing stomata under an optical microscope, measuring major/minor axis values of guard cells, calculating perimeter values of the guard cells with an ellipse model while observing and counting chloroplasts, recording 15 data per leaf, and identifying with parents as controls. For Brassica campestris, stoma guard cells have perimeter smaller than 58.90 micrometer and chloroplast count of 9-11; for Brassica napus, stoma guard cells have perimeter greater than 75.83 micrometer and chloroplast count of 19-20; while, for the to-be-identified material, if more than ten stomata simultaneously satisfy that guard cells have perimeter of 58.90-75.83 micrometer and chloroplast count of 14-16, it is determined that the plant is interspecific hybrids F1 of Brassica campestris and Brassica napus, otherwise it is false hybrid.

The invention relates to a plant distant hybridization technology, in particular to a method for quickly obtaining a large amount of interspecific hybrids of an incompatible wide species and a cultivated species of peanuts through medicine induction after pollination. The method for quickly obtaining the interspecific hybrids of the incompatibility wide species and the cultivated species of the peanuts comprises the following steps of: (1) preparing a hormone mixed solution; (2) collecting incompatible peanut wide species pollen and pollinating a peanut cultivated species female parent; (3) dipping the hormone mixed solution and applying on a base of each pollinated cultivated species female parent flower; and (4) carrying out molecular identification on the harvested seeds. According to the invention, the method can be operated in the field and a large amount of interspecific hybrids can be effectively obtained, a long-winded complicated tissue cultivation step is omitted, and the incompatible hybrids of the peanuts can be simply and easily obtained.

The invention provides a method of obtaining a primula forbesii and primula saxatilis interspecific hybrid by utilizing an immature embryo rescue technology. The method comprises the following steps: selecting primula primula forbesii and primula saxatilis which are originated in China to carry out hybridization; carrying out immature embryo rescue by adopting an ovary culture means; carrying out hybrid morphological identification on obtained progeny materials; selecting the progeny material which has morphological characteristics, such as stems, leaves, flowering phase and inflorescence, similar to parents at the same time, and has novel properties not owned by the parents as a interspecific crossing new variety. The method disclosed by the invention realizes the interspecific distant hybridization of the primula, and obtains a batch of new hybrid germplasms.

The invention relates to a method for creating intergeneric hybridization of radish and turnip and belongs to the technical field of biotechnology breeding. The method specifically includes: sowing parent materials; performing cross pollination during the flowering stage; checking seeds; performing tissue culture to obtain hybrid seedlings; domesticating aseptic seedlings; performing hybrid morphological identification; performing hybrid cytological identification. The method has the advantages that according to the features that the distant hybridization of the radish and Brassica is severe in pre-fertilization barrier and minor in barrier after pollination, bud pollination is used and glycine of certain concentration is smeared to female parent stigmas before pollination to improve the compatibility of the intergeneric hybridization, natural pod bearing is used after the pollination, seed sterile culture is performed by the aid of the tissue culture technology, and the morphological and cytological identification of the obtained hybrid seedlings shows that the hybrid seedlings are actual intergeneric triploid hybrids.

The invention discloses a method for quickly and efficiently cultivating a new orchid variety through hybridization between sword-leaved cymbidium and renanthera coccinea. The method comprises the steps of pollen collection and preservation, artificial pollination and hybrid fruit cultivation, hybrid embryo culture development into rhizomes, rhizome proliferation culture, rhizome differentiation culture, adventitious bud rooting and seedling culture, seedling transplanting and culture, and the like. According to the method provided by the invention, the infrequent bottleneck problem in a flowering period of interspecific hybridization of orchids is solved by using low-temperature preserved, sterile and vigorous pollen, the key problem of dysplasia of interspecific distant hybridization hybrid embryos is solved by using an embryo rescue culture technology, and corresponding rhizome clone proliferation culture is carried out after a single hybrid embryo is developed into a rhizome through sterile culture, so that the defect of long years of traditional hybridization breeding is avoided, and a new orchid variety can be rapidly cultivated.

The invention provides a method for increasing the cross pollination efficiency of E. pungens var. variegata and belongs to the technical field of artificial cross breeding of woody plants. The method comprises the steps: (1) disease and insect pest control; (2) pollen collection; (3) flower thinning; (4) castration, bagging and artificial pollination; (5) crossbreed collection and stratifcation; and (6) sowing, wherein castration is carried out in the full-bloom stage of the E. pungens var. variegata, flower buds which are consistent in growth on each flowering branch of the E. pungens var. variegata are retained, and the rest flower buds are trimmed and removed. The method provided by the invention is not only simple in operation, but also capable of effectively keeping the complete natural structures of flowers and effectively increasing the fruit setting rate of natural crossing from 8% to 65% and high in success rate of cross pollination. By using the method, the castration efficiency of the E. pungens var. variegata is effectively increased, false crossbreeds are avoided, and the purity and fruit setting rate of the crossbreeds are effectively increased.

The invention discloses a molecular marker and method for identifying interspecific hybrids of Chinese cabbage and brassica carinata and tracking separation conditions of A10 and C09 chromosomes of progeny materials, and belongs to the field of plant genetic breeding. The marker is a pair of codominant molecular markers which can be used for identifying the authenticity of the interspecific hybrids of the Chinese cabbage and the brassica carinata, and can also be used for identifying and tracking the separation conditions of self-crossed progeny of distant hybrids, backcross descendants, chromosome addition lines, recombination segregation population and the A10 and C09 chromosomes of plants.