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Pickle and purple cabbage trigenomic species allohexaploid vegetable germplasm and acquisition method

A method of obtaining purple cabbage, which is applied in horticultural methods, plant genetic improvement, botanical equipment and methods, etc., can solve the problem that natural hybridization cannot obtain offspring, etc., and achieve the effect of saving time and strong operability

Active Publication Date: 2015-07-15
ZHEJIANG UNIV
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AI Technical Summary

Problems solved by technology

Studies have found that the natural hybridization of mustard mustard and cabbage can not produce offspring, and the interspecific hybridization of mustard mustard and purple cabbage to obtain new multi-genome polyploid vegetable germplasm has not been reported, and there is no method that can quickly and effectively synthesize Brassica. New polyploid vegetable germplasm with multiple genomes

Method used

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  • Pickle and purple cabbage trigenomic species allohexaploid vegetable germplasm and acquisition method
  • Pickle and purple cabbage trigenomic species allohexaploid vegetable germplasm and acquisition method
  • Pickle and purple cabbage trigenomic species allohexaploid vegetable germplasm and acquisition method

Examples

Experimental program
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Effect test

Embodiment 1

[0029] Materials: In this example, the perennial inbred lines of Brassica oleracea and mustard mustard (B. juncea) were used as materials.

[0030] Step (1): Collect materials

[0031]Use pickled mustard as the female parent and purple cabbage as the male parent to carry out artificial pollination at the bud stage from April to May, and apply it continuously for 2 days to avoid hybridization incompatibility before fertilization, and then take the ovary 7 days after pollination to avoid ovary failure education. At the same time, the ovary developed in the natural state was used as a control, and mature seeds could not be obtained.

[0032] Step (2): Material disinfection

[0033] Clean the extracted ovaries with distilled water first, or soak them in distilled water to avoid dehydration and dryness of the seed pods. Then, on the ultra-clean workbench, disinfect with 75% alcohol for 30 seconds, rinse with sterile water three times, and then soak in 5% sodium hypochlorite solu...

Embodiment 6

[0055] In Example 6, the steps of the three-genome allohexaploid vegetable germplasm of pickled mustard and purple cabbage and the acquisition method of the present invention are the same as those in Example 1, and the differences are shown in Table 3.

[0056] Table 3: Doubling effect of different concentrations of colchicine

[0057] Colchicine concentration (mg / L)

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Abstract

The invention discloses a pickle and purple cabbage tri-genome species allohexaploid vegetable germplasm and an acquisition method, which fill in the blank of lack of brassica trigenomic species allohexaploid vegetable germplasm. The acquisition method comprises the steps that pickle and purple cabbages of brassica vegetable germplasm are cross-fertilized; an ovary is picked at the seventh day after pollination; mature seeds are obtained by embryo rescue; a large quantity of propagated progenies are obtained through subculture and propagation after seed germination; duplication treatment is carried out on a haploid by a liquid culture medium with colchicine to obtain the multi-genome allohexaploid vegetable germplasm. The pickle and purple cabbage tri-genome species allohexaploid vegetable germplasm and the acquisition method, disclosed by the invention, have the advantages of strong operability, simpleness and practicality, save the time for obtaining multi-genome polyploidy material, can be widely used for embryo rescue for interspecies cross of cruciferous brassica vegetable germplasm, and have great significance to cultivation and breeding of new species of special plants.

Description

technical field [0001] The invention relates to three-genome heterohexaploid vegetable germplasm of pickled mustard and purple cabbage and an acquisition method, and relates to a method for artificially synthesizing new multi-genome polyploid germplasm in the technical field. Background technique [0002] Polyploidy is ubiquitous in plants and plays an important role in the process of plant diversification. Polyploidy often causes some morphological and physiological changes in plants, which have the characteristics of gigantism, increased metabolites, increased yield, and enhanced stress resistance. In view of its excellent characteristics, the creation of polyploid has important production and utilization value. At present, polyploid plants are mostly obtained through artificial synthesis. The in-depth study of artificially synthesized new polyploid germplasm and its structure and functional evolution is a hot field for scientists from all over the world to explore, deve...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H1/08A01H4/00
Inventor 陈利萍李俊星饶琳莉
Owner ZHEJIANG UNIV
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