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Method for culturing orchid new variety through hybridization between sword-leaved cymbidium and renanthera coccinea

An intergeneric hybridization and flame orchid technology, applied in the field of plant reproduction, can solve the problems of low success rate of distant hybridization and long breeding cycle, and achieve the effect of avoiding complicated procedures of backcross purification and fast and efficient cultivation.

Inactive Publication Date: 2019-01-04
SOUTH CHINA BOTANICAL GARDEN CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The purpose of the present invention is to develop a method for quickly and efficiently cultivating a new orchid variety that is a hybrid of Jianlan and Flame Orchid, so as to solve the problems of low success rate of distant hybridization and long breeding cycle in orchid hybrid breeding

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] (1) Collection and storage of flame orchid pollen:

[0034] A. Preparation of picking tools and storage utensils: Use a 300mL glass bottle to put toothpicks for picking pollen cubes and 1mL plastic tubes for storing pollen cubes respectively, cover them, and sterilize at 121°C for 30 minutes Backup.

[0035] B. Flame Orchid (male parent) blooms in early March. When the petals of its single flower have just opened, use a sterilized toothpick to gently pick off the medicinal cap of the flower at 8-9 am, and then use the toothpick to stick the pollen block Put the pollen mass on the tip of the toothpick and quickly put it into a clean and sterile 1mL plastic tube, put the pollen mass of one flower in each tube, put the plastic tube with the pollen mass in a sterile glass bottle and put it together Seal well and store at 4°C away from light.

[0036] (2) Artificial pollination and hybrid fruit cultivation: when Jianlan (female parent) blooms in early August, the flame orc...

Embodiment 2

[0043] (1) Collection and storage of flame orchid pollen:

[0044] A. Preparation of picking tools and storage utensils: Use a 300mL glass bottle to put toothpicks for picking pollen cubes and 1mL plastic tubes for storing pollen cubes respectively, cover them, and sterilize at 121°C for 30 minutes Backup.

[0045] B. Flame Orchid (male parent) blooms in early March. When the petals of its single flower have just opened, use a sterilized toothpick to gently pick off the medicinal cap of the flower at 8-9 am, and then use the toothpick to stick the pollen block Put the pollen mass on the tip of the toothpick and quickly put it into a clean and sterile 1mL plastic tube, put the pollen mass of one flower in each tube, put the plastic tube with the pollen mass in a sterile glass bottle and put it together Seal well and store at 6°C away from light.

[0046](2) Artificial pollination and hybrid fruit cultivation: when Jianlan (female parent) blooms in early August, the flame orch...

Embodiment 3

[0053] (1) make material with the adventitious buds of embodiment 1 growing to the seedlings of 5~8cm of seedling height, cut the tender shoots of the seedling lines, and disinfect (tender shoots→dry cotton wipe 1 time→the cotton soaked in 75% ethanol Wipe 1 time → remove excess leaves and cut them into small sections, each section contains a bud point → fully soak in 75% ethanol for 30s → wash 3 times with sterile water → 0.1% HgCl 2 Soak fully for 4-6 minutes→rinse with sterile water for 3 times→dry off the excess water and peel off the bract leaves→0.1%HgCl 2 Fully 1-2min→dry excess water), put the sterilized shoots in the rhizome induction medium GB1, and cultivate them in the dark at 26±2°C for 7 days, then under the light intensity of 1000lx, the light time of 10h / d, Cultivate at 26±2°C for 90 days to induce rhizomes. The rhizome induction medium GB1 contains 1 g of Huabao No. 1, 1 g of Huabao No. 2, ferrous sulfate (FeSO 4 .7H 2 O) 20mg, disodium edetate 20mg, peptone...

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Abstract

The invention discloses a method for quickly and efficiently cultivating a new orchid variety through hybridization between sword-leaved cymbidium and renanthera coccinea. The method comprises the steps of pollen collection and preservation, artificial pollination and hybrid fruit cultivation, hybrid embryo culture development into rhizomes, rhizome proliferation culture, rhizome differentiation culture, adventitious bud rooting and seedling culture, seedling transplanting and culture, and the like. According to the method provided by the invention, the infrequent bottleneck problem in a flowering period of interspecific hybridization of orchids is solved by using low-temperature preserved, sterile and vigorous pollen, the key problem of dysplasia of interspecific distant hybridization hybrid embryos is solved by using an embryo rescue culture technology, and corresponding rhizome clone proliferation culture is carried out after a single hybrid embryo is developed into a rhizome through sterile culture, so that the defect of long years of traditional hybridization breeding is avoided, and a new orchid variety can be rapidly cultivated.

Description

technical field [0001] The invention relates to the technical field of plant propagation, in particular to a method for quickly and efficiently cultivating new orchid varieties by hybridization between Jianlan and Flameorchid. Background technique [0002] Orchid is a famous and precious flower that is popular all over the world. It is one of the most widely cultivated and sold flower species in the world. It has developed particularly rapidly in recent years, with an annual growth rate of more than 10%. One hundred million U.S. dollars. The Netherlands, Thailand, Singapore, the United States, South Korea, and Japan are countries with relatively developed orchid industries, and Thailand is the largest exporter of orchids. At present, most of the orchids produced are moth orchids, dendrobium orchids and Cattleya and other orchids, while the national orchids with traditional Chinese characteristics are not large in scale. [0003] Jianlan is a kind of national orchid, which ...

Claims

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Application Information

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IPC IPC(8): A01H1/02A01H4/00
CPCA01H1/02A01H4/008
Inventor 吴坤林郑枫曾宋君房林李琳
Owner SOUTH CHINA BOTANICAL GARDEN CHINESE ACADEMY OF SCI
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