5 results about "Chromosome number" patented technology

The application discloses a detection system, device and method for embryo chromosomal aneuploidy and parent contamination analysis. The system comprises a database module, an alignment module, an analysis and calculation module and a parent contamination detection module. The analysis and calculation module is used for performing the following steps: calculating the ratio of the number of effective sequences matched to each chromosome to the number of corresponding chromosome sequences in the reference database, performing statistical analysis, obtaining the number of target chromosomes of the sample to be detected, and obtaining the detection result of whether the frequency of alleles observed in the genetic linkage disequilibrium mode is abnormal. The parent contamination detection module comprises a contamination prediction model. The aneuploidy detection and parent contamination analysis of the embryo can be simultaneously realized by one detection.

Th present application relates to the field of intelligent medical treatment, and in particular, to a method, device, and program product for predicting a MET14 skipping mutation. The method comprises: obtaining patient MET gene data, including a chromosome number, physical coordinates, a number of base changes in a reference genome, and a number of mutated base changes; and transmitting the chromosome number, the physical coordinates, the number of base changes in a reference genome, and the number of mutated base changes to a prediction model, so as to obtain a prediction result indicating whether a MET14 skipping mutation is present. The present application can detect classical splicing and hidden splicing of MET14 skipping mutation, improve the mutation detection rate, and has good clinical value.

Th present application relates to the field of intelligent medical treatment, and in particular, to a method, device, and program product for predicting a MET14 skipping mutation. The method comprises: obtaining patient MET gene data, including a chromosome number, physical coordinates, a number of base changes in a reference genome, and a number of mutated base changes; and transmitting the chromosome number, the physical coordinates, the number of base changes in a reference genome, and the number of mutated base changes to a prediction model, so as to obtain a prediction result indicating whether a MET14 skipping mutation is present. The present application can detect classical splicing and hidden splicing of MET14 skipping mutation, improve the mutation detection rate, and has good clinical value.

The present application belongs to the field of animal cytology experimental technology, and specifically discloses a preparation method of crayfish embryo chromosomes, comprising the following steps: taking crayfish embryos in the nauplius stage, immersing in a colchicine solution, culturing in a constant-temperature incubator, discarding supernatant after centrifugation, and obtaining cell mass precipitate; then, low penetration, fixation, sheet preparation, sheet spraying, staining and microscope examination are performed, and the crayfish embryo chromosomes are obtained. The present application first uses crayfish embryos in the nauplius stage to prepare chromosomes, overcomes the limitation of sample quantity, does not need to dissect parent crayfish, and has the advantages of clean division phase, more division phase quantity, clear chromosome quantity and morphology, which is beneficial to the analysis of chromosome type and chromosome heredity; the operation is simple, the preparation period is short, and the cost is low. Finally, the present application can obtain clear somatic cell chromosomes of 188 in the crayfish.

The present application relates to the technical field of plant chromosome preparation, and specifically discloses a method for increasing the number of mitotic metaphase chromosomes of sugarcane or sugarcane relative genus root tips, comprising: soaking sugarcane or sugarcane relative genus stem double bud segments in an indole-3-acetic acid solution with a concentration of 60-120 mg / L, and culturing in sand or peat substrate; root tip pretreatment: culturing for 2-3 days, selecting healthy and tender root tips, and placing the root tips in saturated p-dichlorobenzene aqueous solution for low-temperature treatment at 8-12 DEG C for 3-5 h; using Carnoy's fixative for fixation; enzymolysis of the root tip growth point; smearing the root tip on a glass slide for preparation; and staining treatment. The method for preparing the present application can achieve the fastest time of only 4 days from root tip culture to chromosome observation, has a short cycle, high efficiency, is convenient and fast for sampling, has a large number of metaphase division phases in the chromosomes, clear morphology, a clean glass slide background, and is convenient for chromosome counting observation and karyotype analysis, and has important significance for the development of sugarcane cytogenetics research.