11 results about "Chromosome number" patented technology

The invention belongs to the technical field of chromosome karyotype analysis, and particularly discloses a bougainvillea speetabilis karyotype chromosome number identification method which comprises the following steps: S1, putting a root tip of bougainvillea speetabilis into a mixed solution containing a saturated p-dichlorobenzene solution, 5-20 [mu] L of alpha-bromonaphthalene and 0.008% 15-30 [mu] L of cyclohexaneimide for pretreatment; s2, the root tips are placed in paraformaldehyde fixing liquid to be fixed; s3, after the root tips are cleaned, the root tips are put into an HCl solution; s4, the root tips obtained in the step S3 are placed in a 0.075 mol / L KCl solution to be soaked; s5, dyeing; and S6, carrying out microscopic examination and photographing. The invention also provides an identification system for the bougainvillea glabra karyotype chromosome number. By adopting the identification method and system provided by the invention for identification, chromosomes of bougainvillea speetabilis are dispersed without overlapping, distortion, fracture and main singular marks, and bougainvillea speetabilis chromosome karyotype analysis is facilitated.

The application discloses a detection system, device and method for embryo chromosomal aneuploidy and parent contamination analysis. The system comprises a database module, an alignment module, an analysis and calculation module and a parent contamination detection module. The analysis and calculation module is used for performing the following steps: calculating the ratio of the number of effective sequences matched to each chromosome to the number of corresponding chromosome sequences in the reference database, performing statistical analysis, obtaining the number of target chromosomes of the sample to be detected, and obtaining the detection result of whether the frequency of alleles observed in the genetic linkage disequilibrium mode is abnormal. The parent contamination detection module comprises a contamination prediction model. The aneuploidy detection and parent contamination analysis of the embryo can be simultaneously realized by one detection.

The invention belongs to the technical field of crop variety breeding in modern agriculture, and discloses a method for creating homozygous tetraploid rice by using haploid anther, which makes full use of the characteristic that the number of chromosomes in cells subjected to meiosis is halved, and microspores formed by meiosis are subjected to anther culture at a proper development stage, so that the homozygous tetraploid rice is created. The method comprises the following steps of: a, selecting a diploid rice line with excellent characters as a parent material, and carrying out continuous secondary induction by colchicine to form a callus, and differentiating to emerge seedlings to form a stable homozygous tetraploid rice line; b, selecting anther with microspores in the late uninucleate stage during observation; c, inducing anther calluses; e, differentiating the anther calluses to emerge seedlings; f, seedling callus differentiation and rooting; and g, determining the authenticity of a homozygous tetraploid plant formed by anther culture through morphological and cytological technologies (flow cytometry). The method is simple, low in cost and easy to operate.

Th present application relates to the field of intelligent medical treatment, and in particular, to a method, device, and program product for predicting a MET14 skipping mutation. The method comprises: obtaining patient MET gene data, including a chromosome number, physical coordinates, a number of base changes in a reference genome, and a number of mutated base changes; and transmitting the chromosome number, the physical coordinates, the number of base changes in a reference genome, and the number of mutated base changes to a prediction model, so as to obtain a prediction result indicating whether a MET14 skipping mutation is present. The present application can detect classical splicing and hidden splicing of MET14 skipping mutation, improve the mutation detection rate, and has good clinical value.

The invention discloses a marker for efficiently identifying alfalfa chromosomes and application thereof, belongs to the field of bioinformatics and molecular cytogenetics, and solves the problems that alfalfa is high in ploidy, large in number of chromosomes, small in chromosomes, lack of obvious symbolic characteristics and high in identification difficulty. The marker for efficiently identifying the alfalfa chromosome is used for identifying chromosomes 1-8 of alfalfa, the marker of the alfalfa chromosome is located on the chromosomes 1-8 of the alfalfa, and the marker of the alfalfa chromosome is composed of six pairs of alfalfa specific sequence amplification primers; the marker for efficiently identifying the alfalfa chromosomes can efficiently and accurately identify the 1-8 chromosomes of the alfalfa, can identify the 1-8 chromosomes in the alfalfa at one time, can quickly distinguish different chromosomes, greatly shortens the identification time, improves the working efficiency, is beneficial to understanding the genetic diversity of the alfalfa, and has good application prospects. Individuals with excellent characters can be conveniently selected for hybridization.

The invention relates to the technical field of chromosome data processing, in particular to a chromosome dispersion quality evaluation method, which comprises the following steps: S1, acquiring a chromosome grayscale image; s2, preprocessing the chromosome grayscale image to obtain a preprocessed image; s3, performing target extraction on the preprocessed image to obtain an image target contour; s4, performing filtering and contour extraction processing according to the image target contour to obtain a chromosome dispersion region contour; s5, performing calculation based on the chromosome dispersion area contour to obtain a calculation result of the chromosome dispersion area; s6, performing conversion according to the calculation result of the chromosome dispersion area to obtain a chromosome dispersion quality evaluation result; according to the method, the chromosome dispersion area in the image can be automatically identified and positioned, and meanwhile, the chromosome dispersion area can be accurately calculated, so that the quality of chromosomes can be automatically screened subsequently; the evaluation efficiency of the chromosome dispersion quality is obviously improved, and the working intensity is reduced.

The embodiment of the invention discloses a chromosome specimen processing method and device and electronic equipment, and the method comprises the steps: responding to a first triggering operation, and synchronizing at least one split phase scanning image of a target chromosome specimen scanned by a first system to a second system based on target configuration information; for the at least one split phase scanning image, based on a pre-trained target cutting arrangement model, determining a chromosome karyotype graph corresponding to the split phase scanning image; the chromosome analysis result of the target object to which the chromosome specimen belongs is determined based on the chromosome karyotype graph corresponding to the at least one split phase scanning image, chromosome data transmission of the non-matched system can be automatically carried out according to the configuration information, the data transmission efficiency of the non-matched system is improved, the chromosome karyotype analysis time is shortened, and the analysis efficiency of the chromosome karyotype is improved. And the accuracy and the working efficiency of chromosome scanning analysis are improved.

Th present application relates to the field of intelligent medical treatment, and in particular, to a method, device, and program product for predicting a MET14 skipping mutation. The method comprises: obtaining patient MET gene data, including a chromosome number, physical coordinates, a number of base changes in a reference genome, and a number of mutated base changes; and transmitting the chromosome number, the physical coordinates, the number of base changes in a reference genome, and the number of mutated base changes to a prediction model, so as to obtain a prediction result indicating whether a MET14 skipping mutation is present. The present application can detect classical splicing and hidden splicing of MET14 skipping mutation, improve the mutation detection rate, and has good clinical value.

The present application belongs to the field of animal cytology experimental technology, and specifically discloses a preparation method of crayfish embryo chromosomes, comprising the following steps: taking crayfish embryos in the nauplius stage, immersing in a colchicine solution, culturing in a constant-temperature incubator, discarding supernatant after centrifugation, and obtaining cell mass precipitate; then, low penetration, fixation, sheet preparation, sheet spraying, staining and microscope examination are performed, and the crayfish embryo chromosomes are obtained. The present application first uses crayfish embryos in the nauplius stage to prepare chromosomes, overcomes the limitation of sample quantity, does not need to dissect parent crayfish, and has the advantages of clean division phase, more division phase quantity, clear chromosome quantity and morphology, which is beneficial to the analysis of chromosome type and chromosome heredity; the operation is simple, the preparation period is short, and the cost is low. Finally, the present application can obtain clear somatic cell chromosomes of 188 in the crayfish.

Provided herein are methods of distinguishing between meiotic- and mitotic-origin aneuploidies in certain embodiments. Related systems and computer program products are also provided.

The present application relates to the technical field of plant chromosome preparation, and specifically discloses a method for increasing the number of mitotic metaphase chromosomes of sugarcane or sugarcane relative genus root tips, comprising: soaking sugarcane or sugarcane relative genus stem double bud segments in an indole-3-acetic acid solution with a concentration of 60-120 mg / L, and culturing in sand or peat substrate; root tip pretreatment: culturing for 2-3 days, selecting healthy and tender root tips, and placing the root tips in saturated p-dichlorobenzene aqueous solution for low-temperature treatment at 8-12 DEG C for 3-5 h; using Carnoy's fixative for fixation; enzymolysis of the root tip growth point; smearing the root tip on a glass slide for preparation; and staining treatment. The method for preparing the present application can achieve the fastest time of only 4 days from root tip culture to chromosome observation, has a short cycle, high efficiency, is convenient and fast for sampling, has a large number of metaphase division phases in the chromosomes, clear morphology, a clean glass slide background, and is convenient for chromosome counting observation and karyotype analysis, and has important significance for the development of sugarcane cytogenetics research.