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Method for cultivating corn allopolyploid by using unreduced gamete characteristic of tripsacum dactyloides

A technology of allopolyploidy and rubbing grass, which is applied in the field of plant distant hybridization, can solve the problems of difficult traits of close relatives of maize, narrow genetic basis, and difficulty in producing breakthrough varieties

Active Publication Date: 2014-03-05
SICHUAN AGRI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] In view of the narrow genetic basis in current maize breeding, it is difficult to produce breakthrough varieties, and the reproductive isolation between maize relatives makes it difficult to use the excellent traits of maize relatives in maize breeding. The purpose of the invention is to provide a method for cultivating maize allopolyploid using the unreduced gamete characteristics of Trichograss

Method used

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  • Method for cultivating corn allopolyploid by using unreduced gamete characteristic of tripsacum dactyloides
  • Method for cultivating corn allopolyploid by using unreduced gamete characteristic of tripsacum dactyloides
  • Method for cultivating corn allopolyploid by using unreduced gamete characteristic of tripsacum dactyloides

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0102] Example 1. Cultivation of maize allogenous heptaploids using MTF-1 and the unreduced gamete characteristics of Trichograss

[0103] (1) In Huihe Village Farm, Wenjiang District, Chengdu, Sichuan Province, from the end of March, the corn inbred line Mo17 (Zea mays, 2n=20) was planted in 10 batches with an interval of 10 days, and each batch was 70 in early April, MTF-1 (Tripsazea creammaize T. Su Yuegui, master's thesis of Sichuan Agricultural University, 2009. Publicly available from Sichuan Agricultural University) (see figure 1 ) are planted separately in the field, with a row spacing of 2m×2m, a total of 50 plants;

[0104] (2) At the silking stage, before the MTF-1 ear is silked, put the MTF-1 ear in a kraft paper bag, and after the ear is silked, cut the silk to 1 cm, and use Mo17 as the male parent to MTF- 1 Ear pollination, repeat pollination at least 3 times for each ear, mark on the kraft paper bag after each pollination, and harvest hybrid progeny seeds that ar...

Embodiment 2

[0109] Example 2. Cultivation of maize allo-octoploid using MTF-1 and the unreduced gamete characteristics of Trichograss

[0110] Proceed as follows:

[0111] (1) In the farm of Huihe Village, Wenjiang District, Chengdu, Sichuan Province, the tetraploid perennial maize 9475 (Zea perennis9475, 2n=40) was planted in 7 batches starting from late March (provided free of charge by the Mexico International Maize and Wheat Improvement Center) , 7 days apart in each batch, 10 plants in each batch, planted as a single plant of tetraploid perennial maize, with a row spacing of 2m×2m; in the first ten days of April, the corn allohexaploid MTF-1 ( Tripsazea creammaize T. Su Yuegui, master's thesis of Sichuan Agricultural University, 2009. The public can get it from Sichuan Agricultural University) were planted separately in the field, with a row spacing of 2m×2m, a total of 50 plants;

[0112] (2) At the silking stage, before the MTF-1 ear is silked, put the MTF-1 ear in a kraft paper b...

Embodiment 3

[0117] Example 3, Comparison Test of Determination of Ear and Tassel Characters of Maize Allo-octoploid MTFF-1 and Maize Allohexaploid MTF-1

[0118] Proceed as follows:

[0119] (1) In April 2012, maize allo-octoploid MTFF-1 (Example 2) and maize allohexaploid MTF-1 (provided by Sichuan Agricultural University) were planted on plastic flowers by means of split propagation Pots (caliber 40cm x height 33cm), each material planted 10 pots.

[0120] (2) Determination period and the measured morphological traits

[0121] After the plants entered the tasseling and silking stage, three pots of maize allo-octoploid MTFF-1 and maize allohexaploid MTF-1 were randomly selected, and the filament length, ear length, and inflorescence were measured with a ruler and an Olympus SZX16 fluorescent stereoscope. Long, main stem long floret anther length, main stem short floret anther length, main stem long floret length, main stem short floret length; count the number of spikes on the main ste...

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Abstract

The invention discloses a method for cultivating a corn allopolyploid by using an unreduced gamete characteristic of tripsacum dactyloides, belonging to the field of corn distance hybridization. The method comprises the steps of: hybridizing an MTF-1 (metal-responsive transcription factor) as a female parent with corn or tetraploid perennation corn as a male parent, and then selecting a filial generation plant which is capable of overwintering, namely a new corn allopolyploid, wherein the tiller number of the filial generation plant is more than 15 and the chromosome number of the filial generation plant is a sum of the chromosome number of a female parent and the chromosome number of the male parent. According to the method provided by the invention, the dysgenesis of corn affinis species is overcome and a good inheritance basis is provided for breeding ground-breaking corn species by using the bred corn allopolyploid as a bridge material transfer character. In addition, the method provided by the invention provides a model to breed allopolyploids of other species. The corn allopolyploid bred by the method provided by the invention is perennial, adopts vegetative propagation, and provides a material to corn allopolyploid origin and evolution research and allopolyploid breeding. The method provided by the invention is simple, is short in time, high in efficiency and small in workload.

Description

technical field [0001] The invention belongs to the field of plant distant hybridization methods, and in particular relates to a method for cultivating maize allopolyploids by utilizing the unreduced gamete characteristics of Trichograss grass. Background technique [0002] Polyploidy refers to organisms with three or more chromosome sets in cells, which can be divided into two categories: autopolyploid and allopolyploid. Polyploidy in plants is very common. Many important cultivated crops such as wheat, cotton, peanut, tobacco, sweet potato, potato, etc., and many fruit trees and vegetables are polyploid. Other crops such as corn (Gaut, B.S., etc., Proceedings of the National Academy of Sciences.1997.94 (13): 6809-6814), soybean (Shoemaker, R. et al., Genetics, 1996, 144 (1): 329-338) and other ancestors have also experienced multiple times in evolution process. In the plant kingdom, compared with diploids, polyploids have the effect of increasing cell volume, often showi...

Claims

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Application Information

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IPC IPC(8): A01H1/02A01H1/08C12Q1/68
Inventor 唐祈林荣廷昭程明军苏月贵郑名敏李华雄吴元奇周树峰曹墨菊李志龙蒋伟
Owner SICHUAN AGRI UNIV
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